A Role for Myosin-I in Actin Assembly through Interactions with Vrp1p, Bee1p, and the Arp2/3 Complex

doi:10.1083/jcb.148.2.353

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© The Rockefeller University Press, 0021-9525/2000//353 $5.00
The Journal of Cell Biology, Volume 148, Number 2, , 2000 353-362

Original Article

A Role for Myosin-I in Actin Assembly through Interactions with Vrp1p, Bee1p, and the Arp2/3 Complex

Marie Evangelista^a, Bert M. Klebl^b, Amy H.Y. Tong^a, Bradley A. Webb^a, Thomas Leeuw^b, Ekkehard Leberer^b, Malcolm Whiteway^b, David Y. Thomas^b, and Charles Boone^a

^a Department of Biology, Queen's University, Kingston, Ontario, K7L 3N6, Canada
^b Genetics Division, Institut de Recherche en Biotechnologie, 6100, Avenue Royalmount, Montreal, Quebec, H4P 2R2, Canada
Department of Biology, Earl Hall, Queen's University, Kingston, Ontario, K7L 3N6, Canada.(613) 533-6617(613) 533-6124

boonec{at}biology.queensu.ca

Type I myosins are highly conserved actin-based molecular motorsthat localize to the actin-rich cortex and participate in motilityfunctions such as endocytosis, polarized morphogenesis, andcell migration. The COOH-terminal tail of yeast myosin-I proteins,Myo3p and Myo5p, contains an Src homology domain 3 (SH3) followedby an acidic domain. The myosin-I SH3 domain interacted withboth Bee1p and Vrp1p, yeast homologues of human WASP and WIP,adapter proteins that link actin assembly and signaling molecules.The myosin-I acidic domain interacted with Arp2/3 complex subunits,Arc40p and Arc19p, and showed both sequence similarity and geneticredundancy with the COOH-terminal acidic domain of Bee1p (Las17p),which controls Arp2/3-mediated actin nucleation. These findingssuggest that myosin-I proteins may participate in a diverseset of motility functions through a role in actin assembly.

Key Words: yeast • myosin-I • Arp2/3 • actin assembly • WASP

Abbreviations used in this paper: AD, acidic domain; DIC, differentialinterference contrast; GFP, green fluorescent protein; GST,glutathione-S-transferase; HA, hemagglutinin; MBP, maltose bindingprotein; SH3, Src homology domain 3; WH2, WASP homology domain2.

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D'Agostino, J. L., Goode, B. L. (2005). Dissection of Arp2/3 Complex Actin Nucleation Mechanism and Distinct Roles for Its Nucleation-Promoting Factors in Saccharomyces cerevisiae. Genetics 171: 35-47 [Abstract] [Full Text]
Sirotkin, V., Beltzner, C. C., Marchand, J.-B., Pollard, T. D. (2005). Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast. JCB 170: 637-648 [Abstract] [Full Text]
Krendel, M., Mooseker, M. S. (2005). Myosins: Tails (and Heads) of Functional Diversity. Physiology 20: 239-251 [Abstract] [Full Text]
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Kadota, J., Yamamoto, T., Yoshiuchi, S., Bi, E., Tanaka, K. (2004). Septin Ring Assembly Requires Concerted Action of Polarisome Components, a PAK Kinase Cla4p, and the Actin Cytoskeleton in Saccharomyces cerevisiae. Mol. Biol. Cell 15: 5329-5345 [Abstract] [Full Text]
Oberholzer, U., Iouk, T. L., Thomas, D. Y., Whiteway, M. (2004). Functional Characterization of Myosin I Tail Regions in Candida albicans. Eukaryot Cell 3: 1272-1286 [Abstract] [Full Text]
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Eitzen, G., Wang, L., Thorngren, N., Wickner, W. (2002). Remodeling of organelle-bound actin is required for yeast vacuole fusion. JCB 158: 669-679 [Abstract] [Full Text]
Oberholzer, U., Marcil, A., Leberer, E., Thomas, D. Y., Whiteway, M. (2002). Myosin I Is Required for Hypha Formation in Candidaalbicans. Eukaryot Cell 1: 213-228 [Abstract] [Full Text]
Mochida, J., Yamamoto, T., Fujimura-Kamada, K., Tanaka, K. (2002). The Novel Adaptor Protein, Mti1p, and Vrp1p, a Homolog of Wiskott-Aldrich Syndrome Protein-Interacting Protein (WIP), May Antagonistically Regulate Type I Myosins in Saccharomyces cerevisiae. Genetics 160: 923-934 [Abstract] [Full Text]
Seeley, E. S., Kato, M., Margolis, N., Wickner, W., Eitzen, G. (2002). Genomic Analysis of Homotypic Vacuole Fusion. Mol. Biol. Cell 13: 782-794 [Abstract] [Full Text]
Fazi, B., Cope, M. J. T. V., Douangamath, A., Ferracuti, S., Schirwitz, K., Zucconi, A., Drubin, D. G., Wilmanns, M., Cesareni, G., Castagnoli, L. (2002). Unusual Binding Properties of the SH3 Domain of the Yeast Actin-binding Protein Abp1. STRUCTURAL AND FUNCTIONAL ANALYSIS. J. Biol. Chem. 277: 5290-5298 [Abstract] [Full Text]
Cordonnier, M.-N., Dauzonne, D., Louvard, D., Coudrier, E. (2001). Actin Filaments and Myosin I Alpha Cooperate with Microtubules for the Movement of Lysosomes. Mol. Biol. Cell 12: 4013-4029 [Abstract] [Full Text]
Olazabal, I. M., Machesky, L. M. (2001). Abp1p and cortactin, new "hand-holds" for actin. JCB 154: 679-682 [Abstract] [Full Text]
Drees, B. L., Sundin, B., Brazeau, E., Caviston, J. P., Chen, G.-C., Guo, W., Kozminski, K. G., Lau, M. W., Moskow, J. J., Tong, A., Schenkman, L. R., McKenzie, A. III, Brennwald, P., Longtine, M., Bi, E., Chan, C., Novick, P., Boone, C., Pringle, J. R., Davis, T. N., Fields, S., Drubin, D. G. (2001). A protein interaction map for cell polarity development. JCB 154: 549-576 [Abstract] [Full Text]
Jung, G., Remmert, K., Wu, X., Volosky, J. M., Hammer, J. A. III (2001). The Dictyostelium Carmil Protein Links Capping Protein and the Arp2/3 Complex to Type I Myosins through Their Sh3 Domains. JCB 153: 1479-1498 [Abstract] [Full Text]
Goode, B. L., Rodal, A. A., Barnes, G., Drubin, D. G. (2001). Activation of the Arp2/3 Complex by the Actin Filament Binding Protein Abp1p. JCB 153: 627-634 [Abstract] [Full Text]
Berg, J. S., Powell, B. C., Cheney, R. E. (2001). A Millennial Myosin Census. Mol. Biol. Cell 12: 780-794 [Abstract] [Full Text]
Mayer, B. (2001). SH3 domains: complexity in moderation. J. Cell Sci. 114: 1253-1263 [Abstract]
Smith, M., Swamy, S., Pon, L. (2001). The life cycle of actin patches in mating yeast. J. Cell Sci. 114: 1505-1513 [Abstract]
May, R., Machesky, L. (2001). Phagocytosis and the actin cytoskeleton. J. Cell Sci. 114: 1061-1077 [Abstract]
Lee, W.-L., Bezanilla, M., Pollard, T. D. (2000). Fission Yeast Myosin-I, Myo1p, Stimulates Actin Assembly by Arp2/3 Complex and Shares Functions with Wasp. JCB 151: 789-800 [Abstract] [Full Text]
Weed, S. A., Karginov, A. V., Schafer, D. A., Weaver, A. M., Kinley, A. W., Cooper, J. A., Parsons, J. T. (2000). Cortactin Localization to Sites of Actin Assembly in Lamellipodia Requires Interactions with F-Actin and the Arp2/3 Complex. JCB 151: 29-40 [Abstract] [Full Text]
Machesky, L. M. (2000). The Tails of Two Myosins. JCB 148: 219-222 [Full Text]
Berg, J., Derfler, B., Pennisi, C., Corey, D., Cheney, R. (2000). Myosin-X, a novel myosin with pleckstrin homology domains, associates with regions of dynamic actin. J. Cell Sci. 113: 3439-3451 [Abstract]
Liu, X., Brzeska, H., Korn, E. D. (2000). Functional Analysis of Tail Domains of Acanthamoeba Myosin IC by Characterization of Truncation and Deletion Mutants. J. Biol. Chem. 275: 24886-24892 [Abstract] [Full Text]
Zalevsky, J., Grigorova, I., Mullins, R. D. (2001). Activation of the Arp2/3 Complex by the Listeria ActA Protein. ActA BINDS TWO ACTIN MONOMERS AND THREE SUBUNITS OF THE Arp2/3 COMPLEX. J. Biol. Chem. 276: 3468-3475 [Abstract] [Full Text]
Liu, X., Osherov, N., Yamashita, R., Brzeska, H., Korn, E. D., May, G. S. (2001). Myosin I mutants with only 1% of wild-type actin-activated MgATPase activity retain essential in vivo function(s). Proc. Natl. Acad. Sci. USA 98: 9122-9127 [Abstract] [Full Text]
Lechler, T., Jonsdottir, G. A., Klee, S. K., Pellman, D., Li, R. (2001). A two-tiered mechanism by which Cdc42 controls the localization and activation of an Arp2/3-activating motor complex in yeast. JCB 155: 261-270 [Abstract] [Full Text]