Mass Transport of Proform of a Kdel-Tailed Cysteine Proteinase (Sh-EP) to Protein Storage Vacuoles by Endoplasmic Reticulum-Derived Vesicle Is Involved in Protein Mobilization in Germinating Seeds

doi:10.1083/jcb.148.3.453

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© The Rockefeller University Press, 0021-9525/2000//453 $5.00
The Journal of Cell Biology, Volume 148, Number 3, , 2000 453-464

Original Article

Mass Transport of Proform of a Kdel-Tailed Cysteine Proteinase (Sh-EP) to Protein Storage Vacuoles by Endoplasmic Reticulum–Derived Vesicle Is Involved in Protein Mobilization in Germinating Seeds

Kiminori Toyooka^a, Takashi Okamoto^a, and Takao Minamikawa^a

^a Department of Biological Sciences, Tokyo Metropolitan University, Minami-osawa 1-1, Hachioji, Tokyo, 192-0397 Japan
Department of Biological Sciences, Tokyo Metropolitan University, Minami-osawa, Hachioji, Tokyo, 192-0397 Japan.+81 426-77-2559+81 426-77-2559

okamoto-takashi{at}c.metro-u.ac.jp

A vacuolar cysteine proteinase, designated SH-EP, is expressedin the cotyledon of germinated Vigna mungo seeds and is responsiblefor the degradation of storage proteins. SH-EP is a characteristicvacuolar proteinase possessing a COOH-terminal endoplasmic reticulum(ER) retention sequence, KDEL. In this work, immunocytochemicalanalysis of the cotyledon cells of germinated V. mungo seedswas performed using seven kinds of antibodies to identify theintracellular transport pathway of SH-EP from ER to proteinstorage vacuoles. A proform of SH-EP synthesized in ER accumulatedat the edge or middle region of ER where the transport vesiclewas formed. The vesicle containing a large amount of proSH-EP,termed KV, budded off from ER, bypassed the Golgi complex, andwas sorted to protein storage vacuoles. This massive transportof SH-EP via KV was thought to mediate dynamic protein mobilizationin the cotyledon cells of germinated seeds. We discuss the possibilitiesthat the KDEL sequence of KDEL-tailed vacuolar cysteine proteinasesfunction as an accumulation signal at ER, and that the masstransport of the proteinases by ER-derived KV-like vesicle isinvolved in the protein mobilization of plants.

Key Words: cysteine proteinase • endoplasmic reticulum • intracellular transport • KDEL sequence • protein storage vacuole

Abbreviations used in this paper: KV, KDEL-tailed cysteine proteinase-accumulatingvesicle; PAC, precursor accumulating; PSV, protein storage vacuole;TB, toluidine blue.

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