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© The Rockefeller University Press,
0021-9525/2000//567 $5.00
The Journal of Cell Biology, Volume 148, Number 3,
, 2000 567-578
Original Article |
Requirement for β-Catenin in Anterior-Posterior Axis Formation in Mice
wbirch{at}mdc-berlin.de
The anterior-posterior axis of the mouse embryo is defined before formation of the primitive streak, and axis specification and subsequent anterior development involves signaling from both embryonic ectoderm and visceral endoderm. The Wnt signaling pathway is essential for various developmental processes, but a role in anterior-posterior axis formation in the mouse has not been previously established. β-Catenin is a central player in the Wnt pathway and in cadherin-mediated cell adhesion. We generated β-catenin–deficient mouse embryos and observed a defect in anterior-posterior axis formation at embryonic day 5.5, as visualized by the absence of Hex and Hesx1 and the mislocation of cerberus-like and Lim1 expression. Subsequently, no mesoderm and head structures are generated. Intercellular adhesion is maintained since plakoglobin substitutes for β-catenin. Our data demonstrate that β-catenin function is essential in anterior-posterior axis formation in the mouse, and experiments with chimeric embryos show that this function is required in the embryonic ectoderm.
Key Words: anterior visceral endoderm • Wnt/wingless pathway • cell adhesion • plakoglobin • armadillo
© 2000 The Rockefeller University Press
Abbreviations used in this paper: BMP, bone morphogenetic protein; E, embryonic day; ES, embryonic stem; LEF, lymphoid enhancer factor; TCF, T-cell factor.
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