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© The Rockefeller University Press, 0021-9525/2000//629 $5.00
The Journal of Cell Biology, Volume 148, Number 4, , 2000 629-634

Brief Report

Spatial Separation of Parental Genomes in Preimplantation Mouse Embryos



Wolfgang Mayera, Avril Smitha, Reinald Fundelea, and Thomas Haafa

a Max-Planck-Institut für Molekulare Genetik, 14195 Berlin, Germany
Max-Planck-Institute of Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany.(49/0) 30 8413 1383(49/0) 30 8413 1251

haaf{at}molgen.mpg.de

We have used two different experimental approaches to demonstrate topological separation of parental genomes in preimplantation mouse embryos: mouse eggs fertilized with 5-bromodeoxyuridine (BrdU)-labeled sperm followed by detection of BrdU in early diploid embryos, and differential heterochromatin staining in mouse interspecific hybrid embryos. Separation of chromatin according to parental origin was preserved up to the four-cell embryo stage and then gradually disappeared. In F1 hybrid animals, genome separation was also observed in a proportion of somatic cells. Separate nuclear compartments during preimplantation development, when extreme chromatin remodelling occurs, and possibly in some differentiated cell types, may be associated with epigenetic reprogramming.

Key Words: 5-bromodeoxyuridine • fluorescence in situ hybridization • mouse interspecific hybrids • nuclear architecture • preimplantation embryo

© 2000 The Rockefeller University Press

The authors dedicate this paper to the memory of Wolfgang Mayer who died on August 19, 1999.

Abbreviations used in this paper: BrdU, 5-bromodeoxyuridine; DAPI, 4',6-diamidino-2-phenylindole; FISH, fluorescence in situ hybridization; hCG, human choriogonadotropin; MMU, Mus musculus; MSP, Mus spretus.


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