The Yeast Nuclear Pore Complex: Composition, Architecture, and Transport Mechanism

doi:10.1083/jcb.148.4.635

This Article

Full Text

Full Text (PDF, 931K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Rout, M. P.

Articles by Chait, B. T.

Search for Related Content

PubMed

PubMed Citation

Articles by Rout, M. P.

Articles by Chait, B. T.

Pubmed/NCBI databases

Gene	Genome
GEO Profiles	HomoloGene
Protein

Social Bookmarking

What's this?

© The Rockefeller University Press, 0021-9525/2000/2/635/ $5.00
The Journal of Cell Biology, Volume 148, Number 4, February 21, 2000 635-652

Original Article

The Yeast Nuclear Pore Complex: Composition, Architecture, and Transport Mechanism

Michael P. Rout^a, John D. Aitchison^b, Adisetyantari Suprapto^a, Kelly Hjertaas^b, Yingming Zhao^a, and Brian T. Chait^a
^a The Rockefeller University, New York, NY 10021
^b Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada

Correspondence to: Michael P. Rout, Laboratory of Cellular and Structural Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021. Tel:(212) 327-8135 Fax:(212) 327-7193 E-mail:rout{at}rockvax.rockefeller.edu.

An understanding of how the nuclear pore complex (NPC) mediatesnucleocytoplasmic exchange requires a comprehensive inventoryof the molecular components of the NPC and a knowledge of howeach component contributes to the overall structure of thislarge molecular translocation machine. Therefore, we have takena comprehensive approach to classify all components of the yeastNPC (nucleoporins). This involved identifying all the proteinspresent in a highly enriched NPC fraction, determining whichof these proteins were nucleoporins, and localizing each nucleoporinwithin the NPC. Using these data, we present a map of the moleculararchitecture of the yeast NPC and provide evidence for a Brownianaffinity gating mechanism for nucleocytoplasmic transport.

Key Words: nuclear pore complex, nucleoporins, nucleocytoplasmic transport,mass spectrometry, proteomics

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Wout, P. K., Sattlegger, E., Sullivan, S. M., Maddock, J. R. (2009). Saccharomyces cerevisiae Rbg1 Protein and Its Binding Partner Gir2 Interact on Polyribosomes with Gcn1. Eukaryot Cell 8: 1061-1071 [Abstract] [Full Text]
Flemming, D., Sarges, P., Stelter, P., Hellwig, A., Bottcher, B., Hurt, E. (2009). Two structurally distinct domains of the nucleoporin Nup170 cooperate to tether a subset of nucleoporins to nuclear pores. JCB 185: 387-395 [Abstract] [Full Text]
Onischenko, E., Stanton, L. H., Madrid, A. S., Kieselbach, T., Weis, K. (2009). Role of the Ndc1 interaction network in yeast nuclear pore complex assembly and maintenance. JCB 185: 475-491 [Abstract] [Full Text]
Dawson, T. R., Lazarus, M. D., Hetzer, M. W., Wente, S. R. (2009). ER membrane-bending proteins are necessary for de novo nuclear pore formation. JCB 184: 659-675 [Abstract] [Full Text]
Napetschnig, J., Kassube, S. A., Debler, E. W., Wong, R. W., Blobel, G., Hoelz, A. (2009). Structural and functional analysis of the interaction between the nucleoporin Nup214 and the DEAD-box helicase Ddx19. Proc. Natl. Acad. Sci. USA 106: 3089-3094 [Abstract] [Full Text]
Porter, F. W., Palmenberg, A. C. (2009). Leader-Induced Phosphorylation of Nucleoporins Correlates with Nuclear Trafficking Inhibition by Cardioviruses. J. Virol. 83: 1941-1951 [Abstract] [Full Text]
Liu, H.-L., De Souza, C. P.C., Osmani, A. H., Osmani, S. A. (2009). The Three Fungal Transmembrane Nuclear Pore Complex Proteins of Aspergillus nidulans Are Dispensable in the Presence of an Intact An-Nup84-120 Complex. Mol. Biol. Cell 20: 616-630 [Abstract] [Full Text]
Dange, T., Grunwald, D., Grunwald, A., Peters, R., Kubitscheck, U. (2008). Autonomy and robustness of translocation through the nuclear pore complex: a single-molecule study. JCB 183: 77-86 [Abstract] [Full Text]
Vanrobays, E., Leplus, A., Osheim, Y. N., Beyer, A. L., Wacheul, L., Lafontaine, D. L.J. (2008). TOR regulates the subcellular distribution of DIM2, a KH domain protein required for cotranscriptional ribosome assembly and pre-40S ribosome export. RNA 14: 2061-2073 [Abstract] [Full Text]
Malone, C. D., Falkowska, K. A., Li, A. Y., Galanti, S. E., Kanuru, R. C., LaMont, E. G., Mazzarella, K. C., Micev, A. J., Osman, M. M., Piotrowski, N. K., Suszko, J. W., Timm, A. C., Xu, M.-M., Liu, L., Chalker, D. L. (2008). Nucleus-Specific Importin Alpha Proteins and Nucleoporins Regulate Protein Import and Nuclear Division in the Binucleate Tetrahymena thermophila. Eukaryot Cell 7: 1487-1499 [Abstract] [Full Text]
Martins de Souza, D., Oliveira, B. M., Castro-Dias, E., Winck, F. V., Horiuchi, R. S. O., Baldasso, P. A., Caetano, H. T., Pires, N. K. D., Marangoni, S., Novello, J. C. (2008). The untiring search for the most complete proteome representation: reviewing the methods. Brief Funct Genomic Proteomic 7: 312-321 [Abstract] [Full Text]
Sun, C., Yang, W., Tu, L.-C., Musser, S. M. (2008). Single-molecule measurements of importin {alpha}/cargo complex dissociation at the nuclear pore. Proc. Natl. Acad. Sci. USA 105: 8613-8618 [Abstract] [Full Text]
Zhao, Q., Brkljacic, J., Meier, I. (2008). Two Distinct Interacting Classes of Nuclear Envelope-Associated Coiled-Coil Proteins Are Required for the Tissue-Specific Nuclear Envelope Targeting of Arabidopsis RanGAP. Plant Cell 20: 1639-1651 [Abstract] [Full Text]
Thomsen, R., Saguez, C., Nasser, T., Jensen, T. H. (2008). General, rapid, and transcription-dependent fragmentation of nucleolar antigens in S. cerevisiae mRNA export mutants. RNA 14: 706-716 [Abstract] [Full Text]
Faustino, R. S., Cheung, P., Richard, M. N., Dibrov, E., Kneesch, A. L., Deniset, J. F., Chahine, M. N., Lee, K., Blackwood, D., Pierce, G. N. (2008). Ceramide regulation of nuclear protein import. J. Lipid Res. 49: 654-662 [Abstract] [Full Text]
Resendes, K. K., Rasala, B. A., Forbes, D. J. (2008). Centrin 2 Localizes to the Vertebrate Nuclear Pore and Plays a Role in mRNA and Protein Export. Mol. Cell. Biol. 28: 1755-1769 [Abstract] [Full Text]
Brown, C. R., Kennedy, C. J., Delmar, V. A., Forbes, D. J., Silver, P. A. (2008). Global histone acetylation induces functional genomic reorganization at mammalian nuclear pore complexes. Genes Dev. 22: 627-639 [Abstract] [Full Text]
Theisen, U., Straube, A., Steinberg, G. (2008). Dynamic Rearrangement of Nucleoporins during Fungal "Open" Mitosis. Mol. Biol. Cell 19: 1230-1240 [Abstract] [Full Text]
Williams, R. (2008). Brian Chait: Master of mass spectrometry. JCB 180: 652-653 [Full Text]
Patel, S. S., Rexach, M. F. (2008). Discovering Novel Interactions at the Nuclear Pore Complex Using Bead Halo: A Rapid Method for Detecting Molecular Interactions of High and Low Affinity at Equilibrium. Mol. Cell. Proteomics 7: 121-131 [Abstract] [Full Text]
Terry, L. J., Shows, E. B., Wente, S. R. (2007). Crossing the Nuclear Envelope: Hierarchical Regulation of Nucleocytoplasmic Transport. Science 318: 1412-1416 [Abstract] [Full Text]
Jeudy, S., Schwartz, T. U. (2007). Crystal Structure of Nucleoporin Nic96 Reveals a Novel, Intricate Helical Domain Architecture. J. Biol. Chem. 282: 34904-34912 [Abstract] [Full Text]
Ratner, G. A., Hodel, A. E., Powers, M. A. (2007). Molecular Determinants of Binding between Gly-Leu-Phe-Gly Nucleoporins and the Nuclear Pore Complex. J. Biol. Chem. 282: 33968-33976 [Abstract] [Full Text]
Kramer, A., Ludwig, Y., Shahin, V., Oberleithner, H. (2007). A Pathway Separate from the Central Channel through the Nuclear Pore Complex for Inorganic Ions and Small Macromolecules. J. Biol. Chem. 282: 31437-31443 [Abstract] [Full Text]
Lim, R. Y. H., Fahrenkrog, B., Koser, J., Schwarz-Herion, K., Deng, J., Aebi, U. (2007). Nanomechanical Basis of Selective Gating by the Nuclear Pore Complex. Science 318: 640-643 [Abstract] [Full Text]
Strub, B. R., Eswara, M. B.K., Pierce, J. B., Mangroo, D. (2007). Utp8p Is a Nucleolar tRNA-binding Protein That Forms a Complex with Components of the Nuclear tRNA Export Machinery in Saccharomyces cerevisiae. Mol. Biol. Cell 18: 3845-3859 [Abstract] [Full Text]
Terry, L. J., Wente, S. R. (2007). Nuclear mRNA export requires specific FG nucleoporins for translocation through the nuclear pore complex. JCB 178: 1121-1132 [Abstract] [Full Text]
Kilburn, C. L., Pearson, C. G., Romijn, E. P., Meehl, J. B., Giddings, T. H. Jr., Culver, B. P., Yates, J. R. III, Winey, M. (2007). New Tetrahymena basal body protein components identify basal body domain structure. JCB 178: 905-912 [Abstract] [Full Text]
Sistla, S., Pang, J. V., Wang, C. X., Balasundaram, D. (2007). Multiple Conserved Domains of the Nucleoporin Nup124p and Its Orthologs Nup1p and Nup153 Are Critical for Nuclear Import and Activity of the Fission Yeast Tf1 Retrotransposon. Mol. Biol. Cell 18: 3692-3708 [Abstract] [Full Text]
Scarcelli, J. J., Hodge, C. A., Cole, C. N. (2007). The yeast integral membrane protein Apq12 potentially links membrane dynamics to assembly of nuclear pore complexes. JCB 178: 799-812 [Abstract] [Full Text]
Lewis, A., Felberbaum, R., Hochstrasser, M. (2007). A nuclear envelope protein linking nuclear pore basket assembly, SUMO protease regulation, and mRNA surveillance. JCB 178: 813-827 [Abstract] [Full Text]
Sabri, N., Roth, P., Xylourgidis, N., Sadeghifar, F., Adler, J., Samakovlis, C. (2007). Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport. JCB 178: 557-565 [Abstract] [Full Text]
Higa, M. M., Alam, S. L., Sundquist, W. I., Ullman, K. S. (2007). Molecular Characterization of the Ran-binding Zinc Finger Domain of Nup153. J. Biol. Chem. 282: 17090-17100 [Abstract] [Full Text]
West, M., Hedges, J. B., Lo, K.-Y., Johnson, A. W. (2007). Novel Interaction of the 60S Ribosomal Subunit Export Adapter Nmd3 at the Nuclear Pore Complex. J. Biol. Chem. 282: 14028-14037 [Abstract] [Full Text]
Xu, X. M., Rose, A., Muthuswamy, S., Jeong, S. Y., Venkatakrishnan, S., Zhao, Q., Meier, I. (2007). NUCLEAR PORE ANCHOR, the Arabidopsis Homolog of Tpr/Mlp1/Mlp2/Megator, Is Involved in mRNA Export and SUMO Homeostasis and Affects Diverse Aspects of Plant Development. Plant Cell 19: 1537-1548 [Abstract] [Full Text]
Melcak, I., Hoelz, A., Blobel, G. (2007). Structure of Nup58/45 Suggests Flexible Nuclear Pore Diameter by Intermolecular Sliding. Science 315: 1729-1732 [Abstract] [Full Text]
Ball, J. R., Dimaano, C., Bilak, A., Kurchan, E., Zundel, M. T., Ullman, K. S. (2007). Sequence Preference in RNA Recognition by the Nucleoporin Nup153. J. Biol. Chem. 282: 8734-8740 [Abstract] [Full Text]
Glavy, J. S., Krutchinsky, A. N., Cristea, I. M., Berke, I. C., Boehmer, T., Blobel, G., Chait, B. T. (2007). Cell-cycle-dependent phosphorylation of the nuclear pore Nup107-160 subcomplex. Proc. Natl. Acad. Sci. USA 104: 3811-3816 [Abstract] [Full Text]
Ryan, K. J., Zhou, Y., Wente, S. R. (2007). The Karyopherin Kap95 Regulates Nuclear Pore Complex Assembly into Intact Nuclear Envelopes In Vivo. Mol. Biol. Cell 18: 886-898 [Abstract] [Full Text]
Naim, B., Brumfeld, V., Kapon, R., Kiss, V., Nevo, R., Reich, Z. (2007). Passive and Facilitated Transport in Nuclear Pore Complexes Is Largely Uncoupled. J. Biol. Chem. 282: 3881-3888 [Abstract] [Full Text]
Napetschnig, J., Blobel, G., Hoelz, A. (2007). Crystal structure of the N-terminal domain of the human protooncogene Nup214/CAN. Proc. Natl. Acad. Sci. USA 104: 1783-1788 [Abstract] [Full Text]
Denning, D. P., Rexach, M. F. (2007). Rapid Evolution Exposes the Boundaries of Domain Structure and Function in Natively Unfolded FG Nucleoporins. Mol. Cell. Proteomics 6: 272-282 [Abstract] [Full Text]
Saito, K., Yoshikawa, M., Yano, K., Miwa, H., Uchida, H., Asamizu, E., Sato, S., Tabata, S., Imaizumi-Anraku, H., Umehara, Y., Kouchi, H., Murooka, Y., Szczyglowski, K., Downie, J. A., Parniske, M., Hayashi, M., Kawaguchi, M. (2007). NUCLEOPORIN85 Is Required for Calcium Spiking, Fungal and Bacterial Symbioses, and Seed Production in Lotus japonicus. Plant Cell 19: 610-624 [Abstract] [Full Text]
Presgraves, D. C., Stephan, W. (2007). Pervasive Adaptive Evolution among Interactors of the Drosophila Hybrid Inviability Gene, Nup96. Mol Biol Evol 24: 306-314 [Abstract] [Full Text]
Dong, C.-H., Hu, X., Tang, W., Zheng, X., Kim, Y. S., Lee, B.-h., Zhu, J.-K. (2006). A Putative Arabidopsis Nucleoporin, AtNUP160, Is Critical for RNA Export and Required for Plant Tolerance to Cold Stress. Mol. Cell. Biol. 26: 9533-9543 [Abstract] [Full Text]
Osmani, A. H., Davies, J., Liu, H.-L., Nile, A., Osmani, S. A. (2006). Systematic Deletion and Mitotic Localization of the Nuclear Pore Complex Proteins of Aspergillus nidulans. Mol. Biol. Cell 17: 4946-4961 [Abstract] [Full Text]
Goffin, L., Vodala, S., Fraser, C., Ryan, J., Timms, M., Meusburger, S., Catimel, B., Nice, E. C., Silver, P. A., Xiao, C.-Y., Jans, D. A., Gething, M.-J. H. (2006). The Unfolded Protein Response Transducer Ire1p Contains a Nuclear Localization Sequence Recognized by Multiple beta Importins. Mol. Biol. Cell 17: 5309-5323 [Abstract] [Full Text]
Timney, B. L., Tetenbaum-Novatt, J., Agate, D. S., Williams, R., Zhang, W., Chait, B. T., Rout, M. P. (2006). Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo. JCB 175: 579-593 [Abstract] [Full Text]
Frey, S., Richter, R. P., Gorlich, D. (2006). FG-Rich Repeats of Nuclear Pore Proteins Form a Three-Dimensional Meshwork with Hydrogel-Like Properties. Science 314: 815-817 [Abstract] [Full Text]
Maeshima, K., Yahata, K., Sasaki, Y., Nakatomi, R., Tachibana, T., Hashikawa, T., Imamoto, F., Imamoto, N. (2006). Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins. J. Cell Sci. 119: 4442-4451 [Abstract] [Full Text]
Xylourgidis, N., Roth, P., Sabri, N., Tsarouhas, V., Samakovlis, C. (2006). The nucleoporin Nup214 sequesters CRM1 at the nuclear rim and modulates NF{kappa}B activation in Drosophila. J. Cell Sci. 119: 4409-4419 [Abstract] [Full Text]
Wilkie, G. S., Schirmer, E. C. (2006). Guilt by Association: The Nuclear Envelope Proteome and Disease. Mol. Cell. Proteomics 5: 1865-1875 [Abstract] [Full Text]
Yang, W., Musser, S. M. (2006). Nuclear import time and transport efficiency depend on importin {beta} concentration. JCB 174: 951-961 [Abstract] [Full Text]
Bernad, R., Engelsma, D., Sanderson, H., Pickersgill, H., Fornerod, M. (2006). Nup214-Nup88 Nucleoporin Subcomplex Is Required for CRM1-mediated 60 S Preribosomal Nuclear Export. J. Biol. Chem. 281: 19378-19386 [Abstract] [Full Text]
Lim, R. Y. H., Huang, N.-P., Koser, J., Deng, J., Lau, K. H. A., Schwarz-Herion, K., Fahrenkrog, B., Aebi, U. (2006). Flexible phenylalanine-glycine nucleoporins as entropic barriers to nucleocytoplasmic transport. Proc. Natl. Acad. Sci. USA 103: 9512-9517 [Abstract] [Full Text]
Vizeacoumar, F. J., Vreden, W. N., Aitchison, J. D., Rachubinski, R. A. (2006). Pex19p Binds Pex30p and Pex32p at Regions Required for Their Peroxisomal Localization but Separate from Their Peroxisomal Targeting Signals. J. Biol. Chem. 281: 14805-14812 [Abstract] [Full Text]
Madrid, A. S., Mancuso, J., Cande, W. Z., Weis, K. (2006). The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function. JCB 173: 361-371 [Abstract] [Full Text]
Vizeacoumar, F. J., Vreden, W. N., Fagarasanu, M., Eitzen, G. A., Aitchison, J. D., Rachubinski, R. A. (2006). The Dynamin-like Protein Vps1p of the Yeast Saccharomyces cerevisiae Associates with Peroxisomes in a Pex19p-dependent Manner. J. Biol. Chem. 281: 12817-12823 [Abstract] [Full Text]
Caesar, S., Greiner, M., Schlenstedt, G. (2006). Kap120 Functions as a Nuclear Import Receptor for Ribosome Assembly Factor Rpf1 in Yeast. Mol. Cell. Biol. 26: 3170-3180 [Abstract] [Full Text]
Miao, M., Ryan, K. J., Wente, S. R. (2006). The Integral Membrane Protein Pom34p Functionally Links Nucleoporin Subcomplexes. Genetics 172: 1441-1457 [Abstract] [Full Text]
Butterfield-Gerson, K. L., Scheifele, L. Z., Ryan, E. P., Hopper, A. K., Parent, L. J. (2006). Importin-{beta} Family Members Mediate Alpharetrovirus Gag Nuclear Entry via Interactions with Matrix and Nucleocapsid. J. Virol. 80: 1798-1806 [Abstract] [Full Text]
Devos, D., Dokudovskaya, S., Williams, R., Alber, F., Eswar, N., Chait, B. T., Rout, M. P., Sali, A. (2006). Simple fold composition and modular architecture of the nuclear pore complex. Proc. Natl. Acad. Sci. USA 103: 2172-2177 [Abstract] [Full Text]
Levesque, L., Bor, Y.-C., Matzat, L. H., Jin, L., Berberoglu, S., Rekosh, D., Hammarskjold, M.-L., Paschal, B. M. (2006). Mutations in Tap Uncouple RNA Export Activity from Translocation through the Nuclear Pore Complex. Mol. Biol. Cell 17: 931-943 [Abstract] [Full Text]
Prunuske, A. J., Liu, J., Elgort, S., Joseph, J., Dasso, M., Ullman, K. S. (2006). Nuclear Envelope Breakdown Is Coordinated by Both Nup358/RanBP2 and Nup153, Two Nucleoporins with Zinc Finger Modules. Mol. Biol. Cell 17: 760-769 [Abstract] [Full Text]
Dilworth, D. J., Tackett, A. J., Rogers, R. S., Yi, E. C., Christmas, R. H., Smith, J. J., Siegel, A. F., Chait, B. T., Wozniak, R. W., Aitchison, J. D. (2005). The mobile nucleoporin Nup2p and chromatin-bound Prp20p function in endogenous NPC-mediated transcriptional control. JCB 171: 955-965 [Abstract] [Full Text]
Massague, J., Seoane, J., Wotton, D. (2005). Smad transcription factors. Genes Dev. 19: 2783-2810 [Abstract] [Full Text]
Cristea, I. M., Williams, R., Chait, B. T., Rout, M. P. (2005). Fluorescent Proteins as Proteomic Probes. Mol. Cell. Proteomics 4: 1933-1941 [Abstract] [Full Text]
Soop, T., Ivarsson, B., Bjorkroth, B., Fomproix, N., Masich, S., Cordes, V. C., Daneholt, B. (2005). Nup153 Affects Entry of Messenger and Ribosomal Ribonucleoproteins into the Nuclear Basket during Export. Mol. Biol. Cell 16: 5610-5620 [Abstract] [Full Text]
Belanger, K. D., Gupta, A., MacDonald, K. M., Ott, C. M., Hodge, C. A., Cole, C. M., Davis, L. I. (2005). Nuclear Pore Complex Function in Saccharomyces cerevisiae Is Influenced by Glycosylation of the Transmembrane Nucleoporin Pom152p. Genetics 171: 935-947 [Abstract] [Full Text]
Onischenko, E. A., Gubanova, N. V., Kiseleva, E. V., Hallberg, E. (2005). Cdk1 and Okadaic Acid-sensitive Phosphatases Control Assembly of Nuclear Pore Complexes in Drosophila Embryos. Mol. Biol. Cell 16: 5152-5162 [Abstract] [Full Text]
Palancade, B., Zuccolo, M., Loeillet, S., Nicolas, A., Doye, V. (2005). Pml39, a Novel Protein of the Nuclear Periphery Required for Nuclear Retention of Improper Messenger Ribonucleoparticles. Mol. Biol. Cell 16: 5258-5268 [Abstract] [Full Text]
Leslie, M. (2005). A portrait of the nuclear pore complex. JCB 171: 194-195 [Full Text]
Robinson, M. A., Park, S., Sun, Z.-Y. J., Silver, P. A., Wagner, G., Hogle, J. M. (2005). Multiple Conformations in the Ligand-binding Site of the Yeast Nuclear Pore-targeting Domain of Nup116p. J. Biol. Chem. 280: 35723-35732 [Abstract] [Full Text]
Gorski, S., Misteli, T. (2005). Systems biology in the cell nucleus. J. Cell Sci. 118: 4083-4092 [Abstract] [Full Text]
Scott, R. J., Lusk, C. P., Dilworth, D. J., Aitchison, J. D., Wozniak, R. W. (2005). Interactions between Mad1p and the Nuclear Transport Machinery in the Yeast Saccharomyces cerevisiae. Mol. Biol. Cell 16: 4362-4374 [Abstract] [Full Text]
Kendirgi, F., Rexer, D. J., Alcazar-Roman, A. R., Onishko, H. M., Wente, S. R. (2005). Interaction between the Shuttling mRNA Export Factor Gle1 and the Nucleoporin hCG1: A Conserved Mechanism in the Export of Hsp70 mRNA. Mol. Biol. Cell 16: 4304-4315 [Abstract] [Full Text]
THOMSON, E., TOLLERVEY, D. (2005). Nop53p is required for late 60S ribosome subunit maturation and nuclear export in yeast. RNA 11: 1215-1224 [Abstract] [Full Text]
Kim, M.-K., Claiborn, K. C., Levin, H. L. (2005). The Long Terminal Repeat-Containing Retrotransposon Tf1 Possesses Amino Acids in Gag That Regulate Nuclear Localization and Particle Formation. J. Virol. 79: 9540-9555 [Abstract] [Full Text]
Murthi, A., Hopper, A. K. (2005). Genome-Wide Screen for Inner Nuclear Membrane Protein Targeting in Saccharomyces cerevisiae: Roles for N-Acetylation and an Integral Membrane Protein. Genetics 170: 1553-1560 [Abstract] [Full Text]
Niepel, M., Strambio-de-Castillia, C., Fasolo, J., Chait, B. T., Rout, M. P. (2005). The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly. JCB 170: 225-235 [Abstract] [Full Text]
Lutzmann, M., Kunze, R., Stangl, K., Stelter, P., Toth, K. F., Bottcher, B., Hurt, E. (2005). Reconstitution of Nup157 and Nup145N into the Nup84 Complex. J. Biol. Chem. 280: 18442-18451 [Abstract] [Full Text]
Irwin, B., Aye, M., Baldi, P., Beliakova-Bethell, N., Cheng, H., Dou, Y., Liou, W., Sandmeyer, S. (2005). Retroviruses and yeast retrotransposons use overlapping sets of host genes. Genome Res 15: 641-654 [Abstract] [Full Text]
Hawryluk-Gara, L. A., Shibuya, E. K., Wozniak, R. W. (2005). Vertebrate Nup53 Interacts with the Nuclear Lamina and Is Required for the Assembly of a Nup93-containing Complex. Mol. Biol. Cell 16: 2382-2394 [Abstract] [Full Text]
Menon, B. B., Sarma, N. J., Pasula, S., Deminoff, S. J., Willis, K. A., Barbara, K. E., Andrews, B., Santangelo, G. M. (2005). Reverse recruitment: The Nup84 nuclear pore subcomplex mediates Rap1/Gcr1/Gcr2 transcriptional activation. Proc. Natl. Acad. Sci. USA 102: 5749-5754 [Abstract] [Full Text]
Pardo, M., Nurse, P. (2005). The nuclear rim protein Amo1 is required for proper microtubule cytoskeleton organisation in fission yeast. J. Cell Sci. 118: 1705-1714 [Abstract] [Full Text]
Tackett, A. J., Dilworth, D. J., Davey, M. J., O'Donnell, M., Aitchison, J. D., Rout, M. P., Chait, B. T. (2005). Proteomic and genomic characterization of chromatin complexes at a boundary. JCB 169: 35-47 [Abstract] [Full Text]
Schaper, S., Franke, J., Meijsing, S. H., Ehrenhofer-Murray, A. E. (2005). Nuclear import of the histone acetyltransferase complex SAS-I in Saccharomyces cerevisiae. J. Cell Sci. 118: 1473-1484 [Abstract] [Full Text]
Chen, R., Pan, S., Brentnall, T. A., Aebersold, R. (2005). Proteomic Profiling of Pancreatic Cancer for Biomarker Discovery. Mol. Cell. Proteomics 4: 523-533 [Abstract] [Full Text]
Zhang, Y., Li, X. (2005). A Putative Nucleoporin 96 Is Required for Both Basal Defense and Constitutive Resistance Responses Mediated by suppressor of npr1-1,constitutive 1. Plant Cell 17: 1306-1316 [Abstract] [Full Text]
Varadarajan, P., Mahalingam, S., Liu, P., Ng, S. B. H., Gandotra, S., Dorairajoo, D. S. K., Balasundaram, D. (2005). The Functionally Conserved Nucleoporins Nup124p from Fission Yeast and the Human Nup153 Mediate Nuclear Import and Activity of the Tf1 Retrotransposon and HIV-1 Vpr. Mol. Biol. Cell 16: 1823-1838 [Abstract] [Full Text]
Zhong, H., Takeda, A., Nazari, R., Shio, H., Blobel, G., Yaseen, N. R. (2005). Carrier-independent Nuclear Import of the Transcription Factor PU.1 via RanGTP-stimulated Binding to Nup153. J. Biol. Chem. 280: 10675-10682 [Abstract] [Full Text]
Estruch, F., Hodge, C. A., Rodriguez-Navarro, S., Cole, C. N. (2005). Physical and Genetic Interactions Link the Yeast Protein Zds1p with mRNA Nuclear Export. J. Biol. Chem. 280: 9691-9697 [Abstract] [Full Text]