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© The Rockefeller University Press, 0021-9525/2000//1165 $5.00
The Journal of Cell Biology, Volume 148, Number 6, , 2000 1165-1176

Original Article

Ubinuclein, a Novel Nuclear Protein Interacting with Cellular and Viral Transcription Factors



Sirpa Ahoa, Monique Buissonb, Tiina Pajunena, Young W. Ryooa, Jean-Francois Giotb, Henry Gruffatb, Alain Sergeantb, and Jouni Uittoa

a Department of Dermatology and Cutaneous Biology, Jefferson Medical College, and Jefferson Institute of Molecular Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107
b Laboratoire de Virologie Humaine, U412 Institut National de la Santé et de la Recherche Médicale, F-69346 Lyon cedex 07, France
Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, 233 South 10th Street, BLSB 424, Philadelphia, PA 19107-5541.(215) 923-9354(215) 503-2018

sirpa.aho{at}mail.tju.edu

The major target tissues for Epstein-Barr virus (EBV) infection are B lymphocytes and epithelial cells of the oropharyngeal zone. The product of the EBV BZLF1 early gene, EB1, a member of the basic leucine-zipper family of transcription factors, interacts with both viral and cellular promoters and transcription factors, modulating the reactivation of latent EBV infection. Here, we characterize a novel cellular protein interacting with the basic domains of EB1 and c-Jun, and competing of their binding to the AP1 consensus site. The transcript is present in a wide variety of human adult, fetal, and tumor tissues, and the protein is detected in the nuclei throughout the human epidermis and as either grainy or punctuate nuclear staining in the cultured keratinocytes. The overexpression of tagged cDNA constructs in keratinocytes revealed that the NH2 terminus is essential for the nuclear localization, while the central domain is responsible for the interaction with EB1 and for the phenotype of transfected keratinocytes similar to terminal differentiation. The gene was identified in tail-to-tail orientation with the periplakin gene (PPL) in human chromosome 16p13.3 and in a syntenic region in mouse chromosome 16. We designated this novel ubiquitously expressed nuclear protein as ubinuclein and the corresponding gene as UBN1.

Key Words: Epstein-Barr virus • epidermis • protein–protein interaction • keratinocyte differentiation • chromosome 16

© 2000 The Rockefeller University Press

The sequence data from this article has been deposited with the GenBank/EMBL/DDBJ Data Library under accession nos: AF108453, AF108454, AF108455, AF108456, AF108457, AF108458, and AF108459 (human UBN1 gene); AF108460 and AF108461 (human UBN1 cDNA); AF116523 (mouse Ubn1 gene, 3'-end). Symbols for ubinuclein, UBN1 (human) and Ubn1 (mouse) have been approved by HUGO Nomenclature Committee (www.gene.ucl.ac.uk/nomenclature/).

Abbreviations used in this paper: bZIP, basic leucine-zipper; EBV, Epstein-Barr virus; EB1, product of the EBV BZLF1 early gene; EMSA, electrophoretic mobility shift assay; GST, glutathione S-transferase; IIF, indirect immunofluorescence; PPL, periplakin gene; Ubi-F, full-length ubinuclein construct; Ubi-N, NH2-terminal ubinuclein construct; Ubi-Z, NH2-terminally deleted cDNA; UBN1, ubinuclein gene; UTR, untranslated region; ZAP5, Z-associated protein.


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