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© The Rockefeller University Press, 0021-9525/2000//1223 $5.00
The Journal of Cell Biology, Volume 148, Number 6, , 2000 1223-1230

Original Article

Proteins Needed for Vesicle Budding from the Golgi Complex Are Also Required for the Docking Step of Homotypic Vacuole Fusion



Albert Pricea, William Wicknera, and Christian Ungermanna

a Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755-3844
Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755-3844.(603) 650-1353(603) 650-1701

Vam2p/Vps41p is known to be required for transport vesicles with vacuolar cargo to bud from the Golgi. Like other VAM-encoded proteins, which are needed for homotypic vacuole fusion, we now report that Vam2p and its associated protein Vam6p/Vps39p are needed on each vacuole partner for homotypic fusion. In vitro vacuole fusion occurs in successive steps of priming, docking, and membrane fusion. While priming does not require Vam2p or Vam6p, the functions of these two proteins cannot be fulfilled until priming has occurred, and each is required for the docking reaction which culminates in trans-SNARE pairing. Consistent with their dual function in Golgi vesicle budding and homotypic fusion of vacuoles, approximately half of the Vam2p and Vam6p of the cell are recovered from cell lysates with purified vacuoles.

Key Words: Vps41/Vam2p • Vps39/Vam6p • priming • NSF/Sec18p • {alpha}SNAP-Sec17p

© 2000 The Rockefeller University Press

The website for this laboratory is located at http://www.dartmouth.edu/~wickner

The present address of A. Price is Department of Cell Biology, Sterling Hall of Medicine, C441, Yale University School of Medicine, 333 Cedar Street, PO Box 208002, New Haven, CT 06520-8002.

The present address of C. Ungermann is Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.

Abbreviations used in this paper: SNARE, soluble NSF attachment protein receptor; v- or t-SNARE, vesicle or target SNARE.


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J. Cell Biol. 2000 148: 1-2. [Full Text] [PDF]





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