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© The Rockefeller University Press, 0021-9525/2000//141 $5.00
The Journal of Cell Biology, Volume 149, Number 1, , 2000 141-152

Original Article

Evidence for the Involvement of Kif4 in the Anterograde Transport of L1-Containing Vesicles



Diego Perettia, Leticia Perisa, Silvana Rossob, Santiago Quirogab, and Alfredo Cáceresa

a Instituto Investigación Médica Mercedes y Martín Ferreyra (INIMEC-CONICET), 5000 Córdoba, Argentina
b Departamento Quimica Biológica, Facultad Ciencias Químicas (CIQUIBIC-CONICET), Universidad Nacional de Córdoba, 5000 Córdoba, Argentina
Instituto Investigación Médica Mercedes y Martín Ferreyra, Casilla de Correo 389, 5000 Córdoba, Argentina.054-351-4695163054-351-4681465

acaceres{at}immf.uncor.edu

In this study we present evidence about the cellular functions of KIF4. Using subcellular fractionation techniques and immunoisolation, we have now identified a type of vesicle that associates with KIF4, an NH2-terminal globular motor domain kinesin-like protein. This vesicle is highly concentrated in growth cones and contains L1, a cell adhesion molecule implicated in axonal elongation. It lacks synaptic vesicle markers, receptors for neurotrophins, and membrane proteins involved in growth cone guidance. In cultured neurons, KIF4 and L1 predominantly localize to the axonal shaft and its growth cone. Suppression of KIF4 with antisense oligonucleotides results in the accumulation of L1 within the cell body and in its complete disappearance from axonal tips. In addition, KIF4 suppression prevents L1-enhanced axonal elongation. Taken collectively, our results suggest an important role for KIF4 during neuronal development, a phenomenon which may be related to the anterograde transport of L1-containing vesicles.

Key Words: KIF4 • L1-glycoprotein • microtubule-based transport • axons • neuronal polarity

© 2000 The Rockefeller University Press

Abbreviations used in this paper: APP, amyloid precursor protein; GCP, growth cone particle; KLP, kinesin-like protein; PVDF, polyvinylidene difluoride; SDG, sucrose density gradient; uKHC, conventional kinesin heavy chain.


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J. Cell Biol. 2000 149: 1-2. [Full Text] [PDF]





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