JCB logo
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 585K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krause, M.
Right arrow Articles by Wehland, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krause, M.
Right arrow Articles by Wehland, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/2000//181 $5.00
The Journal of Cell Biology, Volume 149, Number 1, , 2000 181-194

Original Article

Fyn-Binding Protein (Fyb)/Slp-76–Associated Protein (Slap), Ena/Vasodilator-Stimulated Phosphoprotein (Vasp) Proteins and the Arp2/3 Complex Link T Cell Receptor (Tcr) Signaling to the Actin Cytoskeleton



Matthias Krausea, Antonio S. Sechia, Marlies Konradta, David Monnera, Frank B. Gertlerb, and Jürgen Wehlanda

a Department of Cell Biology, Gesellschaft für Biotechnologische Forschung (GBF), D-38124 Braunschweig, Germany
b Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02138-4307
Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, D-38124 Braunschweig, Germany.49-531-6181-44449-531-6181-415

jwe{at}gbf.de

T cell receptor (TCR)-driven activation of helper T cells induces a rapid polarization of their cytoskeleton towards bound antigen presenting cells (APCs). We have identified the Fyn- and SLP-76–associated protein Fyb/SLAP as a new ligand for Ena/ vasodilator-stimulated phosphoprotein (VASP) homology 1 (EVH1) domains. Upon TCR engagement, Fyb/SLAP localizes at the interface between T cells and anti-CD3–coated beads, where Evl, a member of the Ena/VASP family, Wiskott-Aldrich syndrome protein (WASP) and the Arp2/3 complex are also found. In addition, Fyb/SLAP is restricted to lamellipodia of spreading platelets. In activated T cells, Fyb/SLAP associates with Ena/VASP family proteins and is present within biochemical complexes containing WASP, Nck, and SLP-76. Inhibition of binding between Fyb/SLAP and Ena/VASP proteins or WASP and the Arp2/3 complex impairs TCR-dependent actin rearrangement, suggesting that these interactions play a key role in linking T cell signaling to remodeling of the actin cytoskeleton.

Key Words: Evl • EVH1 • WASP • Nck • actin cytoskeleton

© 2000 The Rockefeller University Press

Abbreviations used in this paper: APC, antigen-presenting cell; EST, expressed sequence tag; EVH1, Ena/VASP homology 1; Evl, Ena/VASP-like; Fyb, Fyn-binding protein; GFP, green fluorescent protein; GST, glutathione S-transferase; RT, reverse transcription; SLAP, SLP-76–associated protein; SLP-76, SH2 domain–containing leukocyte protein of 76 kD; TCR, T cell receptor; VASP, vasodilator-stimulated phosphoprotein; WASP, Wiskott-Aldrich syndrome protein.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents