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© The Rockefeller University Press, 0021-9525/2000//683 $5.00
The Journal of Cell Biology, Volume 149, Number 3, , 2000 683-696

Original Article

Analysis of Notch Lacking the Carboxyl Terminus Identified in Drosophila Embryos



Cedric S. Wesleya and Lino Saeza

a Department of Genetics, The Rockefeller University, New York, New York 10021
Department of Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10021.(212) 327-7420(212) 327-8233

wesleyc{at}rockvax.rockefeller.edu

The cell surface receptor Notch is required during development of Drosophila melanogaster for differentiation of numerous tissues. Notch is often required for specification of precursor cells by lateral inhibition and subsequently for differentiation of tissues from these precursor cells. We report here that certain embryonic cells and tissues that develop after lateral inhibition, like the connectives and commissures of the central nervous system, are enriched for a form of Notch not recognized by antibodies made against the intracellular region carboxy-terminal of the CDC10/Ankyrin repeats. Western blotting and immunoprecipitation analyses show that Notch molecules lacking this region are produced during embryogenesis and form protein complexes with the ligand Delta. Experiments with cultured cells indicate that Delta promotes accumulation of a Notch intracellular fragment lacking the carboxyl terminus. Furthermore, Notch lacking the carboxyl terminus functions as a receptor for Delta. These results suggest that Notch activities during development include generation and activity of a truncated receptor we designate N{Delta}Cterm.

Key Words: Notch • Delta • neurogenesis • daughterless • differentiation

© 2000 The Rockefeller University Press

Abbreviations used in this paper: CNS, central nervous system; Dl, Delta; da, daughterless gene; E(spl)C, Enhancer of split Complex genes; N, Notch; Su(H), Suppressor of Hairless protein.


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