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© The Rockefeller University Press, 0021-9525/2000//951 $5.00
The Journal of Cell Biology, Volume 149, Number 4, , 2000 951-968

Original Article

A Direct Interaction of Axonin-1 with Ngcam-Related Cell Adhesion Molecule (Nrcam) Results in Guidance, but Not Growth of Commissural Axons



Dora Fitzlia, Esther T. Stoecklib, Stefan Kunza, Kingsley Siriboura, Christoph Radera, Beat Kunza, Serguei V. Kozlova, Andrea Buchstallera, Robert P. Lanec, Daniel M. Sutera, William J. Dreyerc, and Peter Sondereggera

a Institute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
b Department of Integrative Biology, University of Basel, CH-4051 Basel, Switzerland
c Division of Biology 156-29, California Institute of Technology, Pasadena, California 91125
Institute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland.41-1-635-68-3141-1-635-55-41

pson{at}bioc.unizh.ch

An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti–axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.

Key Words: axon guidance • growth cone • neuronal cell adhesion molecules • immunoglobulin superfamily • signal transduction

© 2000 The Rockefeller University Press

Abbreviations used in this paper: CAM, cell adhesion molecule; Fab, antigen binding fragment; FnIII, fibronectin type III; NgCAM, neuron–glia cell adhesion molecule; NrCAM, NgCAM-related cell adhesion molecule.


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