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© The Rockefeller University Press, 0021-9525/2000//983 $5.00
The Journal of Cell Biology, Volume 149, Number 4, , 2000 983-993

Original Article

Growth Plate Compressions and Altered Hematopoiesis in Collagen X Null Mice



Catherine J. Gressa and Olena Jacenkoa

a University of Pennsylvania School of Veterinary Medicine, Department of Animal Biology, Philadelphia, Pennsylvania 19104-6046
University of Pennsylvania, School of Veterinary Medicine, Department of Animal Biology, Rosenthal Rm. 152, 3800 Spruce Street, Philadelphia, PA 19104-6046.(215) 573-5188(215) 573-9447

jacenko{at}vet.upenn.edu

A variable skeleto-hematopoietic phenotype was observed in collagen X null mice which mirrored the defects in transgenic (Tg) mice with dominant interference collagen X mutations (Jacenko, O., P. LuValle, and B.R. Olsen. 1993. Nature. 365:56–61). Specifically, perinatal lethality was seen in ~10.8% of null mutants at week three after birth, and in another subset by 12 wk. In perinatal lethal mutants, growth plates were compressed, trabecular bone reduced, and hematopoietic aplasia and erythrocyte-filled vascular sinusoids were apparent in marrows. Lymphatic organs, reduced to ~80% that of controls, displayed altered architecture and lymphocyte content. In thymuses, a paucity of cortical CD3+/CD4+/CD8+ lymphocytes was consistent with the marrow's inability to replenish maturing T cells. In spleens, an unaltered T cell distribution was coupled with diffuse staining for IgD+/B220+ B cells, whose reduction was prominent in poorly organized lymphatic nodules. Disorderly arrays of splenic macrophages surrounding periarteriolar lymphatic sheaths and a red pulp depletion further complemented the Tg perinatal lethal phenotype. Moreover, subtle growth plate compressions and hematopoietic changes were seen in all null mice. Data from Tg and null mice implicate the disruption of collagen X function in the observed skeleto-hematopoietic defects, and suggest that hypertrophic cartilage and endochondral skeletogenesis may contribute to the marrow microenvironment prerequisite for blood cell differentiation.

Key Words: endochondral ossification • hypertrophic cartilage • skeletogenesis • marrow • lymphopenia

© 2000 The Rockefeller University Press

Abbreviations used in this paper: EO, endochondral ossification; H&E staining, Harris hematoxylin and eosin Y staining; KO, knockout; PE, phycoerythrin; PI, propidium iodide; SMCD, Schmid metaphyseal chondrodysplasia; SMD, spondylometaphyseal dysplasia; Tg, transgenic; TPE, thymic pan epithelium; WT, wild-type controls.


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