PNG1, a Yeast Gene Encoding a Highly Conserved Peptide:N-glycanase

doi:10.1083/jcb.149.5.1039

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© The Rockefeller University Press, 0021-9525/2000/5/1039/ $5.00
The Journal of Cell Biology, Volume 149, Number 5, May 29, 2000 1039-1052

Original Article

PNG1, a Yeast Gene Encoding a Highly Conserved Peptide:N-glycanase

Tadashi Suzuki^a, Hangil Park^a, Nancy M. Hollingsworth^a, Rolf Sternglanz^a, and William J. Lennarz^a
^a Department of Biochemistry and Cell Biology, Institute of Cell and Developmental Biology, State University of New York at Stony Brook, Stony Brook, New York 11794-5215

Correspondence to: William J. Lennarz, Department of Biochemistry and Cell Biology, Institute of Cell and Developmental Biology, State University of New York at Stony Brook, Stony Brook, NY 11794-5215. Tel:(631) 632-8560 Fax:(631) 632-8575 E-mail:wlennarz{at}notes.cc.sunysb.edu.

It has been proposed that cytoplasmic peptide:N-glycanase (PNGase)may be involved in the proteasome-dependent quality controlmachinery used to degrade newly synthesized glycoproteins thatdo not correctly fold in the ER. However, a lack of informationabout the structure of the enzyme has limited our ability toobtain insight into its precise biological function. A PNGase-defectivemutant (png1-1) was identified by screening a collection ofmutagenized strains for the absence of PNGase activity in cellextracts. The PNG1 gene was mapped to the left arm of chromosomeXVI by genetic approaches and its open reading frame was identified.PNG1 encodes a soluble protein that, when expressed in Escherichiacoli, exhibited PNGase activity. PNG1 may be required for efficientproteasome-mediated degradation of a misfolded glycoprotein.Subcellular localization studies indicate that Png1p is presentin the nucleus as well as the cytosol. Sequencing of expressedsequence tag clones revealed that Png1p is highly conservedin a wide variety of eukaryotes including mammals, suggestingthat the enzyme has an important function.

Key Words: proteasome, de-N-glycosylation, quality control

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