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© The Rockefeller University Press, 0021-9525/2000//1171 $5.00
The Journal of Cell Biology, Volume 149, Number 6, , 2000 1171-1178

Brief Report

The Peroxin Pex19p Interacts with Multiple, Integral Membrane Proteins at the Peroxisomal Membrane



William B. Snydera, Antonius Kollera, A. Jobu Choya, and Suresh Subramania

a Department of Biology, University of California, San Diego, La Jolla, California 92093-0322
Department of Biology, University of California, San Diego, 3230 Bonner Hall, 9500 Gilman Drive, La Jolla, CA 92093-0322.(858) 534-0053(858) 534-2327

ssubramani{at}ucsd.edu

Pex19p is a protein required for the early stages of peroxisome biogenesis, but its precise function and site of action are unknown. We tested the interaction between Pex19p and all known Pichia pastoris Pex proteins by the yeast two-hybrid assay. Pex19p interacted with six of seven known integral peroxisomal membrane proteins (iPMPs), and these interactions were confirmed by coimmunoprecipitation. The interactions were not reduced upon inhibition of new protein synthesis, suggesting that they occur with preexisting, and not newly synthesized, pools of iPMPs. By mapping the domains in six iPMPs that interact with Pex19p and the iPMP sequences responsible for targeting to the peroxisome membrane (mPTSs), we found the majority of these sites do not overlap. Coimmunoprecipitation of Pex19p from fractions that contain peroxisomes or cytosol revealed that the interactions between predominantly cytosolic Pex19p and the iPMPs occur in the organelle pellet that contains peroxisomes. These data, taken together, suggest that Pex19p may have a chaperone-like role at the peroxisome membrane and that it is not the receptor for targeting of iPMPs to the peroxisome.

Key Words: organelle biogenesis • protein localization • peroxin • chaperone • mPTS receptor

© 2000 The Rockefeller University Press

W.B. Snyder and A. Koller contributed equally to this paper.

Abbreviations used in this paper: DSP, dithiobis(succinimidyl propionate); GFP, green fluorescent protein; iPMP(s), integral peroxisome membrane protein(s); mPTS(s), integral peroxisome membrane protein targeting signal(s); PTS(s), peroxisome targeting signal(s).


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J. Cell Biol. 2000 149: 1-2. [Full Text] [PDF]





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