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© The Rockefeller University Press, 0021-9525/2000//1225 $5.00
The Journal of Cell Biology, Volume 149, Number 6, , 2000 1225-1234

Original Article

Isoenzyme-Specific Interaction of Muscle-Type Creatine Kinase with the Sarcomeric M-Line Is Mediated by Nh2-Terminal Lysine Charge-Clamps



Thorsten Hornemanna, Martin Stolza, and Theo Wallimanna

a Swiss Federal Institute of Technology, Institute of Cell Biology, Eidenössisch Technische Hochschule Zürich Hönggerberg, 8093 Zürich, Switzerland

horneman{at}cell.biol.ethz.ch

Creatine kinase (CK) is located in an isoenzyme-specific manner at subcellular sites of energy production and consumption. In muscle cells, the muscle-type CK isoform (MM-CK) specifically interacts with the sarcomeric M-line, while the highly homologous brain-type CK isoform (BB-CK) does not share this property. Sequence comparison revealed two pairs of lysine residues that are highly conserved in M-CK but are not present in B-CK. The role of these lysines in mediating M-line interaction was tested with a set of M-CK and B-CK point mutants and chimeras. We found that all four lysine residues are involved in the isoenzyme-specific M-line interaction, acting pair-wise as strong (K104/K115) and weak interaction sites (K8/K24). An exchange of these lysines in MM-CK led to a loss of M-line binding, whereas the introduction of the very same lysines into BB-CK led to a gain of function by transforming BB-CK into a fully competent M-line–binding protein. The role of the four lysines in MM-CK is discussed within the context of the recently solved x-ray structures of MM-CK and BB-CK.

Key Words: creatine kinase • isoenzyme-specific association • sarcomeric M-line • electrophoretic mobility shift • muscle energetics

© 2000 The Rockefeller University Press

T. Hornemann and M. Stolz contributed equally to this work.

M. Stolz's current address Central Laboratory, PCR-Diagnostics, Wankdorfstrasse 10, 3000 Bern, Switzerland.

Abbreviations used in this paper: BB-CK, brain-type CK isoform; CK, creatine kinase; Mi-CK, mitochondrial promoter; MM-CK, muscle-type CK isoform; PCr, phosphocreatine.


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