JCB logo
PeproTech: Cell Culture Supplements
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 236K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miller, B. J.
Right arrow Articles by Myles, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, B. J.
Right arrow Articles by Myles, D. G.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/2000//1289 $5.00
The Journal of Cell Biology, Volume 149, Number 6, , 2000 1289-1296

Original Article

Normal Fertilization Occurs with Eggs Lacking the Integrin {alpha}6β1 and Is Cd9-Dependent



Brent J. Millera, Elisabeth Georges-Labouessec, Paul Primakoffb, and Diana G. Mylesa

a Department of Molecular and Cellular Biology, University of California Davis, Davis, California 95616
b Department of Cell Biology and Human Anatomy, School of Medicine, University of California Davis, Davis, California 95616
c Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Sante et de la Recherche Medicale, ULP, 67404 Illkirch, C.U. de Strasbourg, France
Department of Molecular and Cellular Biology, University of California Davis, One Shields Avenue, Davis, CA 96516.(530) 752-7522(530) 752-1553

dgmyles{at}ucdavis.edu

Previous results, based on inhibition of fertilization by an anti–{alpha}6 integrin mAb (GoH3), suggest that the {alpha}6β1 integrin on mouse eggs functions as the receptor for sperm (Almeida, E.A., A.P. Huovila, A.E. Sutherland, L.E. Stephens, P.G. Calarco, L.M. Shaw, A.M. Mercurio, A. Sonnenberg, P. Primakoff, D.G. Myles, and J.M. White. 1995. Cell. 81:1095–1104). Because the egg surface tetraspanin CD9 is essential for gamete fusion (Kaji, K., S. Oda, T. Shikano, T. Ohnuki, Y. Uematsu, J. Sakagami, N. Tada, S. Miyazaki, and A. Kudo. 2000. Nat. Genet. 24:279–282; Le Naour, F., E. Rubinstein, C. Jasmin, M. Prenant, and C. Boucheix. 2000. Science. 287:319–321; Miyado, K., G. Yamada, S. Yamada, H. Hasuwa, Y. Nakamura, F. Ryu, K. Suzuki, K. Kosai, K. Inoue, A. Ogura, M. Okabe, and E. Mekada. 2000. Science. 287:321–324) and CD9 is known to associate with integrins, recent models of gamete fusion have posited that egg CD9 acts in association with {alpha}6β1 in fusion (Chen, M.S., K.S. Tung, S.A. Coonrod, Y. Takahashi, D. Bigler, A. Chang, Y. Yamashita, P.W. Kincade, J.C. Herr, and J.M. White. 1999. Proc. Natl. Acad. Sci. USA. 96:11830–11835; Kaji, K., S. Oda, T. Shikano, T. Ohnuki, Y. Uematsu, J. Sakagami, N. Tada, S. Miyazaki, and A. Kudo. 2000. Nat. Genet. 24:279–282; Le Naour, F., E. Rubinstein, C. Jasmin, M. Prenant, and C. Boucheix. 2000. Science. 287:319–321; Miyado, K., G. Yamada, S. Yamada, H. Hasuwa, Y. Nakamura, F. Ryu, K. Su- zuki, K. Kosai, K. Inoue, A. Ogura, M. Okabe, and E. Mekada. 2000. Science. 287:321–324). Using eggs from cultured ovaries of mice lacking the {alpha}6 integrin subunit, we found that the fertilization rate, fertilization index, and sperm binding were not impaired compared with wild-type or heterozygous controls. Furthermore, a reexamination of antibody inhibition, using an assay that better simulates in vivo fertilization conditions, revealed no inhibition of fusion by the GoH3 mAb. We also found that an anti-CD9 mAb completely blocks sperm fusion with either wild-type eggs or eggs lacking {alpha}6β1. Based on these results, we conclude that the {alpha}6β1 integrin is not essential for sperm–egg fusion, and we suggest a new model in which CD9 acts by itself, or interacts with egg protein(s) other than {alpha}6β1, to function in sperm–egg fusion.

Key Words: sperm–egg fusion • tetraspanin • membrane adhesion • oocyte • ovarian cultare

© 2000 The Rockefeller University Press

Abbreviations used in this paper: DAPI, 4',6'-diamidino-2-phenylindole dihydrochloride; FI, fertilization index; FR, fertilization rate.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article


J. Cell Biol. 2000 149: 1-2. [Full Text] [PDF]





  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents