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© The Rockefeller University Press, 0021-9525/2000//1361 $5.00
The Journal of Cell Biology, Volume 149, Number 7, , 2000 1361-1376

Cdc28 Activates Exit from Mitosis in Budding Yeast

^a Department of Physiology, University of California, San Francisco, California 94143-0444
^b Department of Biochemistry, University of California, San Francisco, California 94143-0444
University of California, San Francisco, Department of Physiology, Box 0444, 513 Parnassus Avenue, San Francisco, CA 94143-0444.(415) 476-4929(415) 476-0364

amurray{at}cgl.ucsf.edu

The activity of the cyclin-dependent kinase 1 (Cdk1), Cdc28, inhibits the transition from anaphase to G1 in budding yeast. CDC28-T18V, Y19F (CDC28-VF), a mutant that lacks inhibitory phosphorylation sites, delays the exit from mitosis and is hypersensitive to perturbations that arrest cells in mitosis. Surprisingly, this behavior is not due to a lack of inhibitory phosphorylation or increased kinase activity, but reflects reduced activity of the anaphase-promoting complex (APC), a defect shared with other mutants that lower Cdc28/Clb activity in mitosis. CDC28-VF has reduced Cdc20- dependent APC activity in mitosis, but normal Hct1- dependent APC activity in the G1 phase of the cell cycle. The defect in Cdc20-dependent APC activity in CDC28-VF correlates with reduced association of Cdc20 with the APC. The defects of CDC28-VF suggest that Cdc28 activity is required to induce the metaphase to anaphase transition and initiate the transition from anaphase to G1 in budding yeast.

Key Words: anaphase-promoting complex • Hct1 • Cdc20 • Pds1 • sister chromatid separation

© 2000 The Rockefeller University Press

Abbreviations used in this paper: APC, anaphase-promoting complex; Cdk, cyclin-dependent kinase; DAPI, 4',6-diamidino-2-phenylindole; GFP, green fluorescent protein.

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