Original Article

GAP43, MARCKS, and CAP23 Modulate PI(4,5)P₂ at Plasmalemmal Rafts, and Regulate Cell Cortex Actin Dynamics through a Common Mechanism

Correspondence to: Pico Caroni, Friedrich Miescher Institute, Maulbeerstrasse 66, CH-4058 Basel, Switzerland. Tel:41-61-697-3727 Fax:41-61-697-3976 E-mail:caroni{at}fmi.ch.

The dynamic properties of the cell cortex and its actin cytoskeleton determine important aspects of cell behavior and are a major target of cell regulation. GAP43, myristoylated alanine-rich C kinase substrate (MARCKS), and CAP23 (GMC) are locally abundant, plasmalemma-associated PKC substrates that affect actin cytoskeleton. Their expression correlates with morphogenic processes and cell motility, but their role in cortex regulation has been difficult to define mechanistically. We now show that the three proteins accumulate at rafts, where they codistribute with PI(4,5)P₂, and promote its retention and clustering. Binding and modulation of PI(4,5)P₂ depended on the basic effector domain (ED) of these proteins, and constructs lacking the ED functioned as dominant inhibitors of plasmalemmal PI(4,5)P₂ modulation. In the neuronlike cell line, PC12, NGF- and substrate-induced peripheral actin structures, and neurite outgrowth were greatly augmented by any of the three proteins, and suppressed by ED mutants. Agents that globally mask PI(4,5)P₂ mimicked the effects of GMC on peripheral actin recruitment and cell spreading, but interfered with polarization and process formation. Dominant negative GAP43(ED) also interfered with peripheral nerve regeneration, stimulus-induced nerve sprouting and control of anatomical plasticity at the neuromuscular junction of transgenic mice. These results suggest that GMC are functionally and mechanistically related PI(4,5)P₂ modulating proteins, upstream of actin and cell cortex dynamics regulation.

Key Words: neurite outgrowth, cell spreading, anatomical plasticity, actin recruitment, lipid microdomain

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• Wang, J., Gambhir, A., Hangyas-Mihalyne, G., Murray, D., Golebiewska, U., McLaughlin, S. (2002). Lateral Sequestration of Phosphatidylinositol 4,5-Bisphosphate by the Basic Effector Domain of Myristoylated Alanine-rich C Kinase Substrate Is Due to Nonspecific Electrostatic Interactions. J. Biol. Chem. 277: 34401-34412 [Abstract] [Full Text]  
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• Leaney, J. L., Benians, A., Graves, F. M., Tinker, A. (2002). A Novel Strategy to Engineer Functional Fluorescent Inhibitory G-protein alpha Subunits. J. Biol. Chem. 277: 28803-28809 [Abstract] [Full Text]  
• Mikule, K., Gatlin, J. C., de la Houssaye, B. A., Pfenninger, K. H. (2002). Growth Cone Collapse Induced by Semaphorin 3A Requires 12/15-Lipoxygenase. J. Neurosci. 22: 4932-4941 [Abstract] [Full Text]  
• Niethammer, P., Delling, M., Sytnyk, V., Dityatev, A., Fukami, K., Schachner, M. (2002). Cosignaling of NCAM via lipid rafts and the FGF receptor is required for neuritogenesis. JCB 157: 521-532 [Abstract] [Full Text]  
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• Sukumaran, S. K., Prasadarao, N. V. (2002). Regulation of Protein Kinase C in Escherichia coli K1 Invasion of Human Brain Microvascular Endothelial Cells. J. Biol. Chem. 277: 12253-12262 [Abstract] [Full Text]  
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• Shen, Y., Mani, S., Donovan, S. L., Schwob, J. E., Meiri, K. F. (2002). Growth-Associated Protein-43 Is Required for Commissural Axon Guidance in the Developing Vertebrate Nervous System. J. Neurosci. 22: 239-247 [Abstract] [Full Text]  
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• Wang, J., Arbuzova, A., Hangyas-Mihalyne, G., McLaughlin, S. (2001). The Effector Domain of Myristoylated Alanine-rich C Kinase Substrate Binds Strongly to Phosphatidylinositol 4,5-Bisphosphate. J. Biol. Chem. 276: 5012-5019 [Abstract] [Full Text]  
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