Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 1496K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Laux, T. Articles by Caroni, P. Search for Related Content PubMed PubMed Citation Articles by Laux, T. Articles by Caroni, P. Related Collections Related Article Social Bookmarking                            What's this?

© The Rockefeller University Press, 0021-9525/2000//1455 $5.00
The Journal of Cell Biology, Volume 149, Number 7, , 2000 1455-1472

Gap43, Marcks, and Cap23 Modulate Pi(4,5)p₂ at Plasmalemmal Rafts, and Regulate Cell Cortex Actin Dynamics through a Common Mechanism

^a Friedrich Miescher Institute, CH-4058 Basel, Switzerland
^b Department of Biochemistry, Institute of Medical Sciences, Tokyo, Japan
^c Theodor Kocher Institut, Bern, Switzerland
Friedrich Miescher Institute, Maulbeerstrasse 66, CH-4058 Basel, Switzerland.41-61-697-397641-61-697-3727

caroni{at}fmi.ch

The dynamic properties of the cell cortex and its actin cytoskeleton determine important aspects of cell behavior and are a major target of cell regulation. GAP43, myristoylated alanine-rich C kinase substrate (MARCKS), and CAP23 (GMC) are locally abundant, plasmalemma-associated PKC substrates that affect actin cytoskeleton. Their expression correlates with morphogenic processes and cell motility, but their role in cortex regulation has been difficult to define mechanistically. We now show that the three proteins accumulate at rafts, where they codistribute with PI(4,5)P₂, and promote its retention and clustering. Binding and modulation of PI(4,5)P₂ depended on the basic effector domain (ED) of these proteins, and constructs lacking the ED functioned as dominant inhibitors of plasmalemmal PI(4,5)P₂ modulation. In the neuronlike cell line, PC12, NGF- and substrate-induced peripheral actin structures, and neurite outgrowth were greatly augmented by any of the three proteins, and suppressed by ED mutants. Agents that globally mask PI(4,5)P₂ mimicked the effects of GMC on peripheral actin recruitment and cell spreading, but interfered with polarization and process formation. Dominant negative GAP43(ED) also interfered with peripheral nerve regeneration, stimulus-induced nerve sprouting and control of anatomical plasticity at the neuromuscular junction of transgenic mice. These results suggest that GMC are functionally and mechanistically related PI(4,5)P₂ modulating proteins, upstream of actin and cell cortex dynamics regulation.

Key Words: neurite outgrowth • cell spreading • anatomical plasticity • actin recruitment • lipid microdomain

© 2000 The Rockefeller University Press

CiteULike

Complore

Connotea

Del.icio.us

Digg

Facebook

Reddit

Technorati

Twitter

This article has been cited by other articles:

• Dasgupta, U., Bamba, T., Chiantia, S., Karim, P., Tayoun, A. N. A., Yonamine, I., Rawat, S. S., Rao, R. P., Nagashima, K., Fukusaki, E., Puri, V., Dolph, P. J., Schwille, P., Acharya, J. K., Acharya, U. (2009). Ceramide kinase regulates phospholipase C and phosphatidylinositol 4, 5, bisphosphate in phototransduction. Proc. Natl. Acad. Sci. USA 106: 20063-20068 [Abstract] [Full Text]  
• Hao, M., Bogan, J. S. (2009). Cholesterol Regulates Glucose-stimulated Insulin Secretion through Phosphatidylinositol 4,5-Bisphosphate. J. Biol. Chem. 284: 29489-29498 [Abstract] [Full Text]  
• Riedl, M. S., Braun, P. D., Kitto, K. F., Roiko, S. A., Anderson, L. B., Honda, C. N., Fairbanks, C. A., Vulchanova, L. (2009). Proteomic Analysis Uncovers Novel Actions of the Neurosecretory Protein VGF in Nociceptive Processing. J. Neurosci. 29: 13377-13388 [Abstract] [Full Text]  
• McKenna, J. M. D., Ostap, E. M. (2009). Kinetics of the Interaction of myo1c with Phosphoinositides. J. Biol. Chem. 284: 28650-28659 [Abstract] [Full Text]  
• Micallef, J., Taccone, M., Mukherjee, J., Croul, S., Busby, J., Moran, M. F., Guha, A. (2009). Epidermal Growth Factor Receptor Variant III-Induced Glioma Invasion Is Mediated through Myristoylated Alanine-Rich Protein Kinase C Substrate Overexpression. Cancer Res. 69: 7548-7556 [Abstract] [Full Text]  
• Myohanen, T. T., Garcia-Horsman, J. A., Tenorio-Laranga, J., Mannisto, P. T. (2009). Issues About the Physiological Functions of Prolyl Oligopeptidase Based on Its Discordant Spatial Association With Substrates and Inconsistencies Among mRNA, Protein Levels, and Enzymatic Activity. J. Histochem. Cytochem. 57: 831-848 [Abstract] [Full Text]  
• Bethoney, K. A., King, M. C., Hinshaw, J. E., Ostap, E. M., Lemmon, M. A. (2009). A possible effector role for the pleckstrin homology (PH) domain of dynamin. Proc. Natl. Acad. Sci. USA 106: 13359-13364 [Abstract] [Full Text]  
• Nguyen, T., Lindner, R., Tedeschi, A., Forsberg, K., Green, A., Wuttke, A., Gaub, P., Di Giovanni, S. (2009). NFAT-3 Is a Transcriptional Repressor of the Growth-associated Protein 43 during Neuronal Maturation. J. Biol. Chem. 284: 18816-18823 [Abstract] [Full Text]  
• Nawaz, S., Kippert, A., Saab, A. S., Werner, H. B., Lang, T., Nave, K.-A., Simons, M. (2009). Phosphatidylinositol 4,5-Bisphosphate-Dependent Interaction of Myelin Basic Protein with the Plasma Membrane in Oligodendroglial Cells and Its Rapid Perturbation by Elevated Calcium. J. Neurosci. 29: 4794-4807 [Abstract] [Full Text]  
• Lian, L., Wang, Y., Flick, M., Choi, J., Scott, E. W., Degen, J., Lemmon, M. A., Abrams, C. S. (2009). Loss of pleckstrin defines a novel pathway for PKC-mediated exocytosis. Blood 113: 3577-3584 [Abstract] [Full Text]  
• Nicholson, A. M., Ferreira, A. (2009). Increased Membrane Cholesterol Might Render Mature Hippocampal Neurons More Susceptible to {beta}-Amyloid-Induced Calpain Activation and Tau Toxicity. J. Neurosci. 29: 4640-4651 [Abstract] [Full Text]  
• Hoffmann, E. K., Lambert, I. H., Pedersen, S. F. (2009). Physiology of Cell Volume Regulation in Vertebrates. Physiol. Rev. 89: 193-277 [Abstract] [Full Text]  
• Sason, H., Milgrom, M., Weiss, A. M., Melamed-Book, N., Balla, T., Grinstein, S., Backert, S., Rosenshine, I., Aroeti, B. (2009). Enteropathogenic Escherichia coli Subverts Phosphatidylinositol 4,5-Bisphosphate and Phosphatidylinositol 3,4,5-Trisphosphate upon Epithelial Cell Infection. Mol. Biol. Cell 20: 544-555 [Abstract] [Full Text]  
• Li, H., Chen, G., Zhou, B., Duan, S. (2008). Actin Filament Assembly by Myristoylated, Alanine-rich C Kinase Substrate-Phosphatidylinositol-4,5-diphosphate Signaling Is Critical for Dendrite Branching. Mol. Biol. Cell 19: 4804-4813 [Abstract] [Full Text]  
• Johnson, C. M., Chichili, G. R., Rodgers, W. (2008). Compartmentalization of Phosphatidylinositol 4,5-Bisphosphate Signaling Evidenced Using Targeted Phosphatases. J. Biol. Chem. 283: 29920-29928 [Abstract] [Full Text]  
• Sharma, V. P., DesMarais, V., Sumners, C., Shaw, G., Narang, A. (2008). Immunostaining evidence for PI(4,5)P2 localization at the leading edge of chemoattractant-stimulated HL-60 cells. J. Leukoc. Biol. 84: 440-447 [Abstract] [Full Text]  
• James, D. J., Khodthong, C., Kowalchyk, J. A., Martin, T. F.J. (2008). Phosphatidylinositol 4,5-bisphosphate regulates SNARE-dependent membrane fusion. JCB 182: 355-366 [Abstract] [Full Text]  
• Osawa, S., Funamoto, S., Nobuhara, M., Wada-Kakuda, S., Shimojo, M., Yagishita, S., Ihara, Y. (2008). Phosphoinositides Suppress {gamma}-Secretase in Both the Detergent-soluble and -insoluble States. J. Biol. Chem. 283: 19283-19292 [Abstract] [Full Text]  
• Rittenhouse, A. R. (2008). PIP2 PIP2 Hooray for Maxi K+. JGP 132: 5-8 [Full Text]  
• Vaithianathan, T., Bukiya, A., Liu, J., Liu, P., Asuncion-Chin, M., Fan, Z., Dopico, A. (2008). Direct Regulation of BK Channels by Phosphatidylinositol 4,5-Bisphosphate as a Novel Signaling Pathway. JGP 132: 13-28 [Abstract] [Full Text]  
• Chukkapalli, V., Hogue, I. B., Boyko, V., Hu, W.-S., Ono, A. (2008). Interaction between the Human Immunodeficiency Virus Type 1 Gag Matrix Domain and Phosphatidylinositol-(4,5)-Bisphosphate Is Essential for Efficient Gag Membrane Binding. J. Virol. 82: 2405-2417 [Abstract] [Full Text]  
• Wu, D., Huang, W., Richardson, P. M., Priestley, J. V., Liu, M. (2008). TRPC4 in Rat Dorsal Root Ganglion Neurons Is Increased after Nerve Injury and Is Necessary for Neurite Outgrowth. J. Biol. Chem. 283: 416-426 [Abstract] [Full Text]  
• Huang, C.-L. (2007). Complex roles of PIP2 in the regulation of ion channels and transporters. Am. J. Physiol. Renal Physiol. 293: F1761-F1765 [Abstract] [Full Text]  
• Yuyama, K., Sekino-Suzuki, N., Sanai, Y., Kasahara, K. (2007). Translocation of Activated Heterotrimeric G Protein G{alpha}o to Ganglioside-enriched Detergent-resistant Membrane Rafts in Developing Cerebellum. J. Biol. Chem. 282: 26392-26400 [Abstract] [Full Text]  
• Gamper, N., Shapiro, M. S. (2007). Target-specific PIP2 signalling: how might it work?. J. Physiol. 582: 967-975 [Abstract] [Full Text]  
• Guo, Y., Singleton, P. A., Rowshan, A., Gucek, M., Cole, R. N., Graham, D. R. M., Van Eyk, J. E., Garcia, J. G. N. (2007). Quantitative Proteomics Analysis of Human Endothelial Cell Membrane Rafts: Evidence of MARCKS and MRP Regulation in the Sphingosine 1-Phosphate-induced Barrier Enhancement. Mol. Cell. Proteomics 6: 689-696 [Abstract] [Full Text]  
• Gatlin, J. C., Estrada-Bernal, A., Sanford, S. D., Pfenninger, K. H. (2006). Myristoylated, Alanine-rich C-Kinase Substrate Phosphorylation Regulates Growth Cone Adhesion and Pathfinding. Mol. Biol. Cell 17: 5115-5130 [Abstract] [Full Text]  
• Klausen, T. K., Hougaard, C., Hoffmann, E. K., Pedersen, S. F. (2006). Cholesterol modulates the volume-regulated anion current in Ehrlich-Lettre ascites cells via effects on Rho and F-actin. Am. J. Physiol. Cell Physiol. 291: C757-C771 [Abstract] [Full Text]  
• Fernandes, F., Loura, L. M. S., Fedorov, A., Prieto, M. (2006). Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine. J. Lipid Res. 47: 1521-1525 [Abstract] [Full Text]  
• Guerriero, C. J., Weixel, K. M., Bruns, J. R., Weisz, O. A. (2006). Phosphatidylinositol 5-Kinase Stimulates Apical Biosynthetic Delivery via an Arp2/3-dependent Mechanism. J. Biol. Chem. 281: 15376-15384 [Abstract] [Full Text]  
• Chen, G., Liu, P., Pattar, G. R., Tackett, L., Bhonagiri, P., Strawbridge, A. B., Elmendorf, J. S. (2006). Chromium Activates Glucose Transporter 4 Trafficking and Enhances Insulin-Stimulated Glucose Transport in 3T3-L1 Adipocytes via a Cholesterol-Dependent Mechanism. Mol. Endocrinol. 20: 857-870 [Abstract] [Full Text]  
• Evans, J. H., Murray, D., Leslie, C. C., Falke, J. J. (2006). Specific Translocation of Protein Kinase C{alpha} to the Plasma Membrane Requires Both Ca2+ and PIP2 Recognition by Its C2 Domain. Mol. Biol. Cell 17: 56-66 [Abstract] [Full Text]  
• Gokhale, N. A., Abraham, A., Digman, M. A., Gratton, E., Cho, W. (2005). Phosphoinositide Specificity of and Mechanism of Lipid Domain Formation by Annexin A2-p11 Heterotetramer. J. Biol. Chem. 280: 42831-42840 [Abstract] [Full Text]  
• Lalli, G., Hall, A. (2005). Ral GTPases regulate neurite branching through GAP-43 and the exocyst complex. JCB 171: 857-869 [Abstract] [Full Text]  
• Kegel, K. B., Sapp, E., Yoder, J., Cuiffo, B., Sobin, L., Kim, Y. J., Qin, Z.-H., Hayden, M. R., Aronin, N., Scott, D. L., Isenberg, G., Goldmann, W. H., DiFiglia, M. (2005). Huntingtin Associates with Acidic Phospholipids at the Plasma Membrane. J. Biol. Chem. 280: 36464-36473 [Abstract] [Full Text]  
• Cluzel, C., Saltel, F., Lussi, J., Paulhe, F., Imhof, B. A., Wehrle-Haller, B. (2005). The mechanisms and dynamics of {alpha}v{beta}3 integrin clustering in living cells. JCB 171: 383-392 [Abstract] [Full Text]  
• Meiri, K. F (2005). Lipid rafts and regulation of the cytoskeleton during T cell activation. Phil Trans R Soc B 360: 1663-1672 [Abstract] [Full Text]  
• Hagood, J. S., Prabhakaran, P., Kumbla, P., Salazar, L., MacEwen, M. W., Barker, T. H., Ortiz, L. A., Schoeb, T., Siegal, G. P., Alexander, C. B., Pardo, A., Selman, M. (2005). Loss of Fibroblast Thy-1 Expression Correlates with Lung Fibrogenesis. Am. J. Pathol. 167: 365-379 [Abstract] [Full Text]  
• Marin-Vicente, C., Gomez-Fernandez, J. C., Corbalan-Garcia, S. (2005). The ATP-dependent Membrane Localization of Protein Kinase C{alpha} Is Regulated by Ca2+ Influx and Phosphatidylinositol 4,5-Bisphosphate in Differentiated PC12 Cells. Mol. Biol. Cell 16: 2848-2861 [Abstract] [Full Text]  
• Aoyagi, K., Sugaya, T., Umeda, M., Yamamoto, S., Terakawa, S., Takahashi, M. (2005). The Activation of Exocytotic Sites by the Formation of Phosphatidylinositol 4,5-Bisphosphate Microdomains at Syntaxin Clusters. J. Biol. Chem. 280: 17346-17352 [Abstract] [Full Text]  
• Santuccione, A., Sytnyk, V., Leshchyns'ka, I., Schachner, M. (2005). Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59fyn and to enhance neurite outgrowth. JCB 169: 341-354 [Abstract] [Full Text]  
• Golub, T., Caroni, P. (2005). PI(4,5)P2-dependent microdomain assemblies capture microtubules to promote and control leading edge motility. JCB 169: 151-165 [Abstract] [Full Text]  
• Spires, T. L., Molnar, Z., Kind, P. C., Cordery, P. M., Upton, A. L., Blakemore, C., Hannan, A. J. (2005). Activity-dependent Regulation of Synapse and Dendritic Spine Morphology in Developing Barrel Cortex Requires Phospholipase C-{beta}1 Signalling. Cereb Cortex 15: 385-393 [Abstract] [Full Text]  
• Milosevic, I., Sorensen, J. B., Lang, T., Krauss, M., Nagy, G., Haucke, V., Jahn, R., Neher, E. (2005). Plasmalemmal Phosphatidylinositol-4,5-Bisphosphate Level Regulates the Releasable Vesicle Pool Size in Chromaffin Cells. J. Neurosci. 25: 2557-2565 [Abstract] [Full Text]  
• Bodin, S., Soulet, C., Tronchere, H., Sie, P., Gachet, C., Plantavid, M., Payrastre, B. (2005). Integrin-dependent interaction of lipid rafts with the actin cytoskeleton in activated human platelets. J. Cell Sci. 118: 759-769 [Abstract] [Full Text]  
• Gonzalez-Quevedo, R., Shoffer, M., Horng, L., Oro, A. E. (2005). Receptor tyrosine phosphatase-dependent cytoskeletal remodeling by the hedgehog-responsive gene MIM/BEG4. JCB 168: 453-463 [Abstract] [Full Text]  
• Wang, Y. J., Li, W. H., Wang, J., Xu, K., Dong, P., Luo, X., Yin, H. L. (2004). Critical role of PIP5KI{gamma}87 in InsP3-mediated Ca2+ signaling. JCB 167: 1005-1010 [Abstract] [Full Text]  
• Huang, S., Lifshitz, L., Patki-Kamath, V., Tuft, R., Fogarty, K., Czech, M. P. (2004). Phosphatidylinositol-4,5-Bisphosphate-Rich Plasma Membrane Patches Organize Active Zones of Endocytosis and Ruffling in Cultured Adipocytes. Mol. Cell. Biol. 24: 9102-9123 [Abstract] [Full Text]  
• Disatnik, M.-H., Boutet, S. C., Pacio, W., Chan, A. Y., Ross, L. B., Lee, C. H., Rando, T. A. (2004). The bi-directional translocation of MARCKS between membrane and cytosol regulates integrin-mediated muscle cell spreading. J. Cell Sci. 117: 4469-4479 [Abstract] [Full Text]  
• Klopfenstein, D. R., Vale, R. D. (2004). The Lipid Binding Pleckstrin Homology Domain in UNC-104 Kinesin is Necessary for Synaptic Vesicle Transport in Caenorhabditis elegans. Mol. Biol. Cell 15: 3729-3739 [Abstract] [Full Text]  
• Richards, D. A., de Paola, V., Caroni, P., Gahwiler, B. H., McKinney, R. A. (2004). AMPA-receptor activation regulates the diffusion of a membrane marker in parallel with dendritic spine motility in the mouse hippocampus. J. Physiol. 558: 503-512 [Abstract] [Full Text]  
• Chodniewicz, D., Alteraifi, A. M., Zhelev, D. V. (2004). Experimental Evidence for the Limiting Role of Enzymatic Reactions in Chemoattractant-induced Pseudopod Extension in Human Neutrophils. J. Biol. Chem. 279: 24460-24466 [Abstract] [Full Text]  
• James, D. J., Salaun, C., Brandie, F. M., Connell, J. M. C., Chamberlain, L. H. (2004). Neomycin Prevents the Wortmannin Inhibition of Insulin-stimulated Glut4 Translocation and Glucose Transport in 3T3-L1 Adipocytes. J. Biol. Chem. 279: 20567-20570 [Abstract] [Full Text]  
• Liu, P., Leffler, B. J., Weeks, L. K., Chen, G., Bouchard, C. M., Strawbridge, A. B., Elmendorf, J. S. (2004). Sphingomyelinase activates GLUT4 translocation via a cholesterol-dependent mechanism. Am. J. Physiol. Cell Physiol. 286: C317-C329 [Abstract] [Full Text]  
• Chen, C., Weisz, O. A., Stolz, D. B., Watkins, S. C., Montelaro, R. C. (2004). Differential Effects of Actin Cytoskeleton Dynamics on Equine Infectious Anemia Virus Particle Production. J. Virol. 78: 882-891 [Abstract] [Full Text]  
• Wong, W., Schlichter, L. C. (2004). Differential Recruitment of Kv1.4 and Kv4.2 to Lipid Rafts by PSD-95. J. Biol. Chem. 279: 444-452 [Abstract] [Full Text]  
• Salli, U., Saito, N., Stormshak, F. (2003). Spatiotemporal Interactions of Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) Protein with the Actin Cytoskeleton and Exocytosis of Oxytocin upon Prostaglandin F2{alpha} Stimulation of Bovine Luteal Cells. Biol. Reprod. 69: 2053-2058 [Abstract] [Full Text]  
• Kwik, J., Boyle, S., Fooksman, D., Margolis, L., Sheetz, M. P., Edidin, M. (2003). Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4,5-bisphosphate-dependent organization of cell actin. Proc. Natl. Acad. Sci. USA 100: 13964-13969 [Abstract] [Full Text]  
• Aikawa, Y., Martin, T. F.J. (2003). ARF6 regulates a plasma membrane pool of phosphatidylinositol(4,5)bisphosphate required for regulated exocytosis. JCB 162: 647-659 [Abstract] [Full Text]  
• Bompard, G., Martin, M., Roy, C., Vignon, F., Freiss, G. (2003). Membrane targeting of protein tyrosine phosphatase PTPL1 through its FERM domain via binding to phosphatidylinositol 4,5-biphosphate. J. Cell Sci. 116: 2519-2530 [Abstract] [Full Text]  
• Becart, S., Setterblad, N., Ostrand-Rosenberg, S., Ono, S. J., Charron, D., Mooney, N. (2003). Intracytoplasmic domains of MHC class II molecules are essential for lipid-raft-dependent signaling. J. Cell Sci. 116: 2565-2575 [Abstract] [Full Text]  
• Huang, Z.-y., Wu, Y., Burke, S. P., Gutmann, D. H. (2003). The 43,000 Growth-associated Protein Functions as a Negative Growth Regulator in Glioma. Cancer Res. 63: 2933-2939 [Abstract] [Full Text]  
• Leshchyns'ka, I., Sytnyk, V., Morrow, J. S., Schachner, M. (2003). Neural cell adhesion molecule (NCAM) association with PKC{beta}2 via {beta}I spectrin is implicated in NCAM-mediated neurite outgrowth. JCB 161: 625-639 [Abstract] [Full Text]  
• Pierini, L. M., Eddy, R. J., Fuortes, M., Seveau, S., Casulo, C., Maxfield, F. R. (2003). Membrane Lipid Organization Is Critical for Human Neutrophil Polarization. J. Biol. Chem. 278: 10831-10841 [Abstract] [Full Text]  
• Pastor, A. M., Mentis, G. Z., De la Cruz, R. R., Diaz, E., Navarrete, R. (2003). Increased Electrotonic Coupling in Spinal Motoneurons After Transient Botulinum Neurotoxin Paralysis in the Neonatal Rat. J. Neurophysiol. 89: 793-805 [Abstract] [Full Text]  
• Irwin, N., Chao, S., Goritchenko, L., Horiuchi, A., Greengard, P., Nairn, A. C., Benowitz, L. I. (2002). Nerve growth factor controls GAP-43 mRNA stability via the phosphoprotein ARPP-19. Proc. Natl. Acad. Sci. USA 99: 12427-12431 [Abstract] [Full Text]  
• Wang, J., Gambhir, A., Hangyas-Mihalyne, G., Murray, D., Golebiewska, U., McLaughlin, S. (2002). Lateral Sequestration of Phosphatidylinositol 4,5-Bisphosphate by the Basic Effector Domain of Myristoylated Alanine-rich C Kinase Substrate Is Due to Nonspecific Electrostatic Interactions. J. Biol. Chem. 277: 34401-34412 [Abstract] [Full Text]  
• van Rheenen, J., Jalink, K. (2002). Agonist-induced PIP2 Hydrolysis Inhibits Cortical Actin Dynamics: Regulation at a Global but not at a Micrometer Scale. Mol. Biol. Cell 13: 3257-3267 [Abstract] [Full Text]  
• Leaney, J. L., Benians, A., Graves, F. M., Tinker, A. (2002). A Novel Strategy to Engineer Functional Fluorescent Inhibitory G-protein alpha Subunits. J. Biol. Chem. 277: 28803-28809 [Abstract] [Full Text]  
• Mikule, K., Gatlin, J. C., de la Houssaye, B. A., Pfenninger, K. H. (2002). Growth Cone Collapse Induced by Semaphorin 3A Requires 12/15-Lipoxygenase. J. Neurosci. 22: 4932-4941 [Abstract] [Full Text]  
• Niethammer, P., Delling, M., Sytnyk, V., Dityatev, A., Fukami, K., Schachner, M. (2002). Cosignaling of NCAM via lipid rafts and the FGF receptor is required for neuritogenesis. JCB 157: 521-532 [Abstract] [Full Text]  
• Rauch, M. E., Ferguson, C. G., Prestwich, G. D., Cafiso, D. S. (2002). Myristoylated Alanine-rich C Kinase Substrate (MARCKS) Sequesters Spin-labeled Phosphatidylinositol 4,5-Bisphosphate in Lipid Bilayers. J. Biol. Chem. 277: 14068-14076 [Abstract] [Full Text]  
• Gutmann, D. H., Hedrick, N. M., Li, J., Nagarajan, R., Perry, A., Watson, M. A. (2002). Comparative Gene Expression Profile Analysis of Neurofibromatosis 1-associated and Sporadic Pilocytic Astrocytomas. Cancer Res. 62: 2085-2091 [Abstract] [Full Text]  
• Sukumaran, S. K., Prasadarao, N. V. (2002). Regulation of Protein Kinase C in Escherichia coli K1 Invasion of Human Brain Microvascular Endothelial Cells. J. Biol. Chem. 277: 12253-12262 [Abstract] [Full Text]  
• Ziegler, W. H., Tigges, U., Zieseniss, A., Jockusch, B. M. (2002). A Lipid-regulated Docking Site on Vinculin for Protein Kinase C. J. Biol. Chem. 277: 7396-7404 [Abstract] [Full Text]  
• Shen, Y., Mani, S., Donovan, S. L., Schwob, J. E., Meiri, K. F. (2002). Growth-Associated Protein-43 Is Required for Commissural Axon Guidance in the Developing Vertebrate Nervous System. J. Neurosci. 22: 239-247 [Abstract] [Full Text]  
• Gutmann, D. H., Wu, Y. L., Hedrick, N. M., Zhu, Y., Guha, A., Parada, L. F. (2001). Heterozygosity for the neurofibromatosis 1 (NF1) tumor suppressor results in abnormalities in cell attachment, spreading and motility in astrocytes. Hum Mol Genet 10: 3009-3016 [Abstract] [Full Text]  
• Friedl, P., Borgmann, S., Brocker, E.-B. (2001). Amoeboid leukocyte crawling through extracellular matrix: lessons from the Dictyostelium paradigm of cell movement. J. Leukoc. Biol. 70: 491-509 [Abstract] [Full Text]  
• Herreros, J., Ng, T., Schiavo, G. (2001). Lipid Rafts Act as Specialized Domains for Tetanus Toxin Binding and Internalization into Neurons. Mol. Biol. Cell 12: 2947-2960 [Abstract] [Full Text]  
• Andrews, R. K., Munday, A. D., Mitchell, C. A., Berndt, M. C. (2001). Interaction of calmodulin with the cytoplasmic domain of the platelet membrane glycoprotein Ib-IX-V complex. Blood 98: 681-687 [Abstract] [Full Text]  
• Marshall, J. G., Booth, J. W., Stambolic, V., Mak, T., Balla, T., Schreiber, A. D., Meyer, T., Grinstein, S. (2001). Restricted Accumulation of Phosphatidylinositol 3-Kinase Products in a Plasmalemmal Subdomain during Fc{gamma} Receptor-Mediated Phagocytosis. JCB 153: 1369-1380 [Abstract] [Full Text]  
• Sechi, A., Wehland, J (2000). The actin cytoskeleton and plasma membrane connection: PtdIns(4,5)P(2) influences cytoskeletal protein activity at the plasma membrane. J. Cell Sci. 113: 3685-3695 [Abstract]  
• Wang, J., Arbuzova, A., Hangyas-Mihalyne, G., McLaughlin, S. (2001). The Effector Domain of Myristoylated Alanine-rich C Kinase Substrate Binds Strongly to Phosphatidylinositol 4,5-Bisphosphate. J. Biol. Chem. 276: 5012-5019 [Abstract] [Full Text]  
• Rebres, R. A., Green, J. M., Reinhold, M. I., Ticchioni, M., Brown, E. J. (2001). Membrane Raft Association of CD47 Is Necessary for Actin Polymerization and Protein Kinase C theta Translocation in Its Synergistic Activation of T Cells. J. Biol. Chem. 276: 7672-7680 [Abstract] [Full Text]  
• Yarmola, E. G., Edison, A. S., Lenox, R. H., Bubb, M. R. (2001). Actin Filament Cross-linking by MARCKS. CHARACTERIZATION OF TWO ACTIN-BINDING SITES WITHIN THE PHOSPHORYLATION SITE DOMAIN. J. Biol. Chem. 276: 22351-22358 [Abstract] [Full Text]  
• Rizzo, M. A., Kraft, C. A., Watkins, S. C., Levitan, E. S., Romero, G. (2001). Agonist-dependent Traffic of Raft-associated Ras and Raf-1 Is Required for Activation of the Mitogen-activated Protein Kinase Cascade. J. Biol. Chem. 276: 34928-34933 [Abstract] [Full Text]  
• Bruns, J. R., Ellis, M. A., Jeromin, A., Weisz, O. A. (2002). Multiple Roles for Phosphatidylinositol 4-Kinase in Biosynthetic Transport in Polarized Madin-Darby Canine Kidney Cells. J. Biol. Chem. 277: 2012-2018 [Abstract] [Full Text]  
• Niethammer, P., Delling, M., Sytnyk, V., Dityatev, A., Fukami, K., Schachner, M. (2002). Cosignaling of NCAM via lipid rafts and the FGF receptor is required for neuritogenesis. JCB 157: 521-532 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents