JCB logo
Fluorescence In Vivo Endomicroscopy
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 11 July 2000. doi:10.1083/jcb.150.1.1
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 625K)
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yucel, J. K.
Right arrow Articles by Philp, A. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yucel, J. K.
Right arrow Articles by Philp, A. V.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*Nucleotide
*Protein*UniGene
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© The Rockefeller University Press, 0021-9525/2000/7/1/ $5.00
The Journal of Cell Biology, Volume 150, Number 1, July 10, 2000 1-12

Original Article

CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila

Jennifer K. Yucela,f, Janet D. Marszaleka, J. Richard McIntoshf, Lawrence S.B. Goldsteinb,c,d, Don W. Clevelanda,c,e, and Alastair Valentine Philpb,c
a Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, California 92093
b Howard Hughes Medical Institute, University of California, San Diego, La Jolla, California 92093
c Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093
d Department of Pharmacology, University of California, San Diego, La Jolla, California 92093
e Department of Medicine, University of California, San Diego, La Jolla, California 92093
f Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309

Correspondence to: Don W. Cleveland, Ludwig Institute for Cancer Research, 9500 Gilman Drive, La Jolla, CA 92093. Tel:(858) 534-7811 Fax:(858) 534-7659 E-mail:dcleveland{at}ucsd.edu.

CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chromosomes. However, unlike CENP-E, which leaves the centromere/kinetochore region at the end of anaphase A, the CENP-meta protein remains associated with the centromeric/kinetochore region of the chromosome during all stages of the Drosophila cell cycle. P-element–mediated disruption of the CENP-meta gene leads to late larval/pupal stage lethality with incomplete chromosome alignment at metaphase. Complete removal of CENP-meta from the female germline leads to lethality in early embryos resulting from defects in metaphase chromosome alignment. Real-time imaging of these mutants with GFP-labeled chromosomes demonstrates that CENP-meta is required for the maintenance of chromosomes at the metaphase plate, demonstrating that the functions required to establish and maintain chromosome congression have distinguishable requirements.

Key Words: kinetochore, kinesin-like protein, CENP-E, chromosome congression, spindle assembly checkpoint


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents