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© The Rockefeller University Press, 0021-9525/2000//1 $5.00
The Journal of Cell Biology, Volume 150, Number 1, , 2000 1-12

CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila

^a Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, California 92093
^b Howard Hughes Medical Institute, University of California, San Diego, La Jolla, California 92093
^c Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093
^d Department of Pharmacology, University of California, San Diego, La Jolla, California 92093
^e Department of Medicine, University of California, San Diego, La Jolla, California 92093
^f Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309
Ludwig Institute for Cancer Research, 9500 Gilman Drive, La Jolla, CA 92093.(858) 534-7659(858) 534-7811

dcleveland{at}ucsd.edu

CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chromosomes. However, unlike CENP-E, which leaves the centromere/kinetochore region at the end of anaphase A, the CENP-meta protein remains associated with the centromeric/kinetochore region of the chromosome during all stages of the Drosophila cell cycle. P-element–mediated disruption of the CENP-meta gene leads to late larval/pupal stage lethality with incomplete chromosome alignment at metaphase. Complete removal of CENP-meta from the female germline leads to lethality in early embryos resulting from defects in metaphase chromosome alignment. Real-time imaging of these mutants with GFP-labeled chromosomes demonstrates that CENP-meta is required for the maintenance of chromosomes at the metaphase plate, demonstrating that the functions required to establish and maintain chromosome congression have distinguishable requirements.

Key Words: kinetochore • kinesin-like protein • CENP-E • chromosome congression • spindle assembly checkpoint

© 2000 The Rockefeller University Press

Dr. Yucel's current address is Genomica, 1745 38th St., Boulder, CO 80301.

Dr. Philp's current address is CEST, 5 Berners Road, Islington, London N1 0PW, UK.

Abbreviations used in this paper: aa, amino acids; nt(s), nucleotide(s); utr, untranslated region.

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