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Published online 10 July 2000. doi:10.1083/jcb.150.1.243
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© The Rockefeller University Press, 0021-9525/2000//243 $5.00
The Journal of Cell Biology, Volume 150, Number 1, , 2000 243-252

Original Article

Intercellular Adhesion Molecule-5 Induces Dendritic Outgrowth by Homophilic Adhesion



Li Tiana, Henrietta Nymana, Patrick Kilgannonb, Yoshihiro Yoshiharac, Kensaku Morid, Leif C. Anderssone, Sami Kaukinena,f, Heikki Rauvalaa,f, W. Michael Gallatinb, and Carl G. Gahmberga

a Department of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland
b ICOS Corporation, Bothell, Washington 98021
c Laboratory for Neurobiology of Synapse, Brain Science Institute, The Institute of Physical and Chemical Research (RIKEN), Wako-City, Saitama 351, Japan
d Laboratory for Neuronal Recognition Molecules, Brain Science Institute, RIKEN, Wako-City, Saitama 351, Japan
e Haartman Institute, Department of Pathology, University of Helsinki, Helsinki 00014, Finland
f Laboratory of Molecular Neurobiology, Institute of Biotechnology, University of Helsinki, Helsinki 00014, Finland
Department of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland.358-9-19159068358-9-19159028

carl.gahmberg{at}helsinki.fi

Intercellular adhesion molecule-5 (ICAM-5) is a dendritically polarized membrane glycoprotein in telencephalic neurons, which shows heterophilic binding to leukocyte β2-integrins. Here, we show that the human ICAM-5 protein interacts in a homophilic manner through the binding of the immunoglobulin domain 1 to domains 4–5. Surface coated ICAM-5-Fc promoted dendritic outgrowth and arborization of ICAM- 5–expressing hippocampal neurons. During dendritogenesis in developing rat brain, ICAM-5 was in monomer form, whereas in mature neurons it migrated as a high molecular weight complex. The findings indicate that its homophilic binding activity was regulated by nonmonomer/monomer transition. Thus, ICAM-5 displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes.

Key Words: leukocyte • integrin • adhesion • dendrite • neuron

© 2000 The Rockefeller University Press

Abbreviations used in this paper: AxCAM, axon-associated cell adhesion molecule; D, immunoglobulin domain; DenCAM, dendrite-associated cell adhesion molecule; E19, embryonic day 19; ICAM, intercellular adhesion molecule; MAP-2, microtubule-associated protein-2; P, postnatal day; WT, wild-type.


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