JCB logo
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 10 July 2000. doi:10.1083/jcb.150.1.243
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 469K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tian, L.
Right arrow Articles by Gahmberg, C. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tian, L.
Right arrow Articles by Gahmberg, C. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/2000//243 $5.00
The Journal of Cell Biology, Volume 150, Number 1, , 2000 243-252

Original Article

Intercellular Adhesion Molecule-5 Induces Dendritic Outgrowth by Homophilic Adhesion



Li Tiana, Henrietta Nymana, Patrick Kilgannonb, Yoshihiro Yoshiharac, Kensaku Morid, Leif C. Anderssone, Sami Kaukinena,f, Heikki Rauvalaa,f, W. Michael Gallatinb, and Carl G. Gahmberga

a Department of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland
b ICOS Corporation, Bothell, Washington 98021
c Laboratory for Neurobiology of Synapse, Brain Science Institute, The Institute of Physical and Chemical Research (RIKEN), Wako-City, Saitama 351, Japan
d Laboratory for Neuronal Recognition Molecules, Brain Science Institute, RIKEN, Wako-City, Saitama 351, Japan
e Haartman Institute, Department of Pathology, University of Helsinki, Helsinki 00014, Finland
f Laboratory of Molecular Neurobiology, Institute of Biotechnology, University of Helsinki, Helsinki 00014, Finland
Department of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland.358-9-19159068358-9-19159028

carl.gahmberg{at}helsinki.fi

Intercellular adhesion molecule-5 (ICAM-5) is a dendritically polarized membrane glycoprotein in telencephalic neurons, which shows heterophilic binding to leukocyte β2-integrins. Here, we show that the human ICAM-5 protein interacts in a homophilic manner through the binding of the immunoglobulin domain 1 to domains 4–5. Surface coated ICAM-5-Fc promoted dendritic outgrowth and arborization of ICAM- 5–expressing hippocampal neurons. During dendritogenesis in developing rat brain, ICAM-5 was in monomer form, whereas in mature neurons it migrated as a high molecular weight complex. The findings indicate that its homophilic binding activity was regulated by nonmonomer/monomer transition. Thus, ICAM-5 displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes.

Key Words: leukocyte • integrin • adhesion • dendrite • neuron

© 2000 The Rockefeller University Press

Abbreviations used in this paper: AxCAM, axon-associated cell adhesion molecule; D, immunoglobulin domain; DenCAM, dendrite-associated cell adhesion molecule; E19, embryonic day 19; ICAM, intercellular adhesion molecule; MAP-2, microtubule-associated protein-2; P, postnatal day; WT, wild-type.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents