JCB logo
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
A correction to this article has been published: J. Cell Biol. 150 (6) 1515
Published online 7 August 2000. doi:10.1083/jcb.150.3.499
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 924K)
Right arrow PPT slides of all figures
Right arrow Correction (v150,p1515)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rogers, G. C.
Right arrow Articles by Scholey, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rogers, G. C.
Right arrow Articles by Scholey, J. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/2000//499 $5.00
The Journal of Cell Biology, Volume 150, Number 3, , 2000 499-512

Original Article

A Kinesin-Related Protein, Krp180, Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division



Gregory C. Rogersa, Kitty K. Chuia, Edwin W. Leea, Karen P. Wedamana, David J. Sharpa, Gina Hollanda, Robert L. Morrisa, and Jonathan M. Scholeya

a Section of Molecular and Cellular Biology, University of California at Davis, Davis, California 95616
Section of Molecular and Cellular Biology, 149 Briggs Hall, University of California at Davis, Davis, CA 95616.(530) 752-7522(530) 752-2271

jmscholey{at}ucdavis.edu

We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP180, in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP180 concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP180 is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.

Key Words: Xklp2 • kinesin • sea urchin • mitosis • {gamma}-tubulin

© 2000 The Rockefeller University Press

Abbreviations used in this paper: DAPI, 4'-6-diamidino-2-phenylindole; GST, glutathione-S-transferase; KRP, kinesin-related protein; MAP, microtubule-associated protein; MTs, microtubules; NEBD, nuclear envelope breakdown; ORF, open reading frame.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents