Nicotinic Acid Adenine Dinucleotide Phosphate (Naadp+) Is an Essential Regulator of T-Lymphocyte Ca2+-Signaling

doi:10.1083/jcb.150.3.581

Published online 7 August 2000. doi:10.1083/jcb.150.3.581

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© The Rockefeller University Press, 0021-9525/2000//581 $5.00
The Journal of Cell Biology, Volume 150, Number 3, , 2000 581-588

Original Article

Nicotinic Acid Adenine Dinucleotide Phosphate (Naadp⁺) Is an Essential Regulator of T-Lymphocyte Ca²⁺-Signaling

Ingeborg Berg^a, Barry V.L. Potter^b, Georg W. Mayr^a, and Andreas H. Guse^a

^a Institute of Medical Biochemistry and Molecular Biology, Division of Cellular Signal Transduction, University of Hamburg, D-20146 Hamburg, Germany
^b Wolfson Laboratory for Medicinal Chemistry, Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom
University of Hamburg, Institute for Medical Biochemistry and Molecular Biology, Div. Cellular Signal Transduction, Grindelallee 117, IV, D-20146 Hamburg, Germany.49-40-42838-427549-40-42838-4276

guse{at}uke.uni-hamburg.de

Microinjection of human Jurkat T-lymphocytes with nicotinicacid adenine dinucleotide phosphate (NAADP⁺) dose-dependentlystimulated intracellular Ca²⁺-signaling. At a concentrationof 10 nM NAADP⁺ evoked repetitive and long-lasting Ca²⁺-oscillationsof low amplitude, whereas at 50 and 100 nM, a rapid and highinitial Ca²⁺-peak followed by trains of smaller Ca²⁺-oscillationswas observed. Higher concentrations of NAADP⁺ (1 and 10 µM)gradually reduced the initial Ca²⁺-peak, and a complete self-inactivationof Ca²⁺-signals was seen at 100 µM. The effect of NAADP⁺was specific as it was not observed with nicotinamide adeninedinucleotide phosphate. Both inositol 1,4,5-trisphosphate–and cyclic adenosine diphosphoribose–mediated Ca²⁺-signalingwere efficiently inhibited by coinjection of a self-inactivatingconcentration of NAADP⁺. Most importantly, microinjection ofa self-inactivating concentration of NAADP⁺ completely abolishedsubsequent stimulation of Ca²⁺-signaling via the T cell receptor/CD3complex, indicating that a functional NAADP⁺ Ca²⁺-release systemis essential for T-lymphocyte Ca²⁺-signaling.

Key Words: cyclic ADP-ribose • inositol 1,4,5-trisphosphate • T cell activation • signal transduction • ryanodine receptor

Abbreviations used in this paper: [Ca²⁺]_i, intracellular Ca²⁺concentration; cADPR, cyclic ADP-ribose; Ins(1,4,5)P₃, D-myo-inositol1,4,5-trisphosphate; InsP₃-R, Ins(1,4,5)P₃ receptor; Ins(1,4,6)PS₃,D-myo-inositol 1,4,6-trisphosphorothioate; NAADP⁺, nicotinicacid adenine dinucleotide phosphate; NADP⁺, nicotinamide adeninedinucleotide phosphate; RyR, ryanodine receptor(s); TCR/CD3,T cell receptor/CD3.

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