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Published online 7 August 2000. doi:10.1083/jcb.150.3.643
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© The Rockefeller University Press, 0021-9525/2000//643 $5.00
The Journal of Cell Biology, Volume 150, Number 3, , 2000 643-656

Original Article

Characterization of Palladin, a Novel Protein Localized to Stress Fibers and Cell Adhesions



Mana M. Parasta and Carol A. Oteyb

a Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908
b Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
Department of Cell and Molecular Physiology, CB #7545, UNC-CH, Chapel Hill, NC 27599.(919) 966-6927(919) 966-8239

carol_otey{at}med.unc.edu

Here, we describe the identification of a novel phosphoprotein named palladin, which colocalizes with {alpha}-actinin in the stress fibers, focal adhesions, cell–cell junctions, and embryonic Z-lines. Palladin is expressed as a 90–92-kD doublet in fibroblasts and coimmunoprecipitates in a complex with {alpha}-actinin in fibroblast lysates. A cDNA encoding palladin was isolated by screening a mouse embryo library with mAbs. Palladin has a proline-rich region in the NH2-terminal half of the molecule and three tandem Ig C2 domains in the COOH-terminal half. In Northern and Western blots of chick and mouse tissues, multiple isoforms of palladin were detected. Palladin expression is ubiquitous in embryonic tissues, and is downregulated in certain adult tissues in the mouse. To probe the function of palladin in cultured cells, the Rcho-1 trophoblast model was used. Palladin expression was observed to increase in Rcho-1 cells when they began to assemble stress fibers. Antisense constructs were used to attenuate expression of palladin in Rcho-1 cells and fibroblasts, and disruption of the cytoskeleton was observed in both cell types. At longer times after antisense treatment, fibroblasts became fully rounded. These results suggest that palladin is required for the normal organization of the actin cytoskeleton and focal adhesions.

Key Words: focal adhesion • adherens junction • microfilament • {alpha}-actinin • trophoblast

© 2000 The Rockefeller University Press

Abbreviations used in this paper: CEF, chick embryo fibroblast; EST, expressed sequence tag; IP, immunoprecipitation; MEF, mouse embryo fibroblast; pen/strep, 100 U/ml penicillin G, and 100 µg/ml streptomycin; RT-PCR, reverse transcriptase–PCR; VASP, vasodilator-stimulated phosphoprotein.


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