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Published online 7 August 2000. doi:10.1083/jcb.150.3.657
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© The Rockefeller University Press, 0021-9525/2000//657 $5.00
The Journal of Cell Biology, Volume 150, Number 3, , 2000 657-666

Original Article

An Oligodendrocyte Cell Adhesion Molecule at the Site of Assembly of the Paranodal Axo-Glial Junction



Steven Taita, Frank Gunn-Moorea, J. Martin Collinsona, Jeffery Huangb, Catherine Lubetzkic, Liliana Pedrazab, Diane L. Shermana, David R. Colmanb, and Peter J. Brophya

a Department of Preclinical Veterinary Sciences, University of Edinburgh, Edinburgh EH9 1QH, United Kingdom
b Department of Biochemistry and Molecular Biology, Program in Cell Adhesion, Mount Sinai School of Medicine, New York, New York 10029
c INSERM U-495, Biologie des Interactions Neurones/Glie, Hôpital de la Salpetrière, 75651 Paris Cedex 13, France
Department of Preclinical Veterinary Sciences, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK.44-131-650-657644-131-650-6107

peter.brophy{at}ed.ac.uk

Two major isoforms of the cell adhesion molecule neurofascin NF186 and NF155 are expressed in the central nervous system (CNS). We have investigated their roles in the assembly of the node of Ranvier and show that they are targeted to distinct domains at the node. At the onset of myelination, NF186 is restricted to neurons, whereas NF155 localizes to oligodendrocytes, the myelin-forming glia of the CNS. Coincident with axon ensheathment, NF155 clusters at the paranodal regions of the myelin sheath where it localizes in apposition to the axonal adhesion molecule paranodin/contactin-associated protein (Caspr1), which is a constituent of the septate junction-like axo-glial adhesion zone. Immunoelectron microscopy confirmed that neurofascin is a glial component of the paranodal axo-glial junction. Concentration of NF155 with Caspr1 at the paranodal junctions of peripheral nerves is also a feature of Schwann cells. In Shiverer mutant mice, which assemble neither compact CNS myelin nor normal paranodes, NF155 (though largely retained at the cell body) is also distributed at ectopic sites along axons, where it colocalizes with Caspr1. Hence, NF155 is the first glial cell adhesion molecule to be identified in the paranodal axo-glial junction, where it likely interacts with axonal proteins in close association with Caspr1.

Key Words: glia • node of Ranvier • neurofascin • myelination • paranode

© 2000 The Rockefeller University Press

Abbreviations used in this paper: Caspr, contactin-associated protein; CNS, central nervous system; DRG, dorsal root ganglion; FNIII, fibronectin type III; MAG, myelin-associated glycoprotein; PNS, peripheral nervous system; RT, reverse transcriptase.


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