Binding of the Mex67p/Mtr2p Heterodimer to Fxfg, Glfg, and Fg Repeat Nucleoporins Is Essential for Nuclear mRNA Export

doi:10.1083/jcb.150.4.695

Published online 21 August 2000. doi:10.1083/jcb.150.4.695

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© The Rockefeller University Press, 0021-9525/2000//695 $5.00
The Journal of Cell Biology, Volume 150, Number 4, , 2000 695-706

Original Article

Binding of the Mex67p/Mtr2p Heterodimer to Fxfg, Glfg, and Fg Repeat Nucleoporins Is Essential for Nuclear mRNA Export

Katja Sträßer^a, Jochen Baßler^a, and Ed Hurt^a

^a BZH, Biochemie-Zentrum Heidelberg, D-69120 Heidelberg, Germany
BZH, Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.49 6221 54 43 6949 6221 54 41 73

cg5{at}ix.urz.uni-heidelberg.de

It is not known how Mex67p and Mtr2p, which form a heterodimeressential for mRNA export, transport mRNPs through the nuclearpore. Here, we show that the Mex67p/Mtr2p complex binds to allof the repeat types (GLFG, FXFG, and FG) found in nucleoporins.For this interaction, complex formation between Mex67p and Mtr2phas to occur. MEX67 and MTR2 also genetically interact withdifferent types of repeat nucleoporins, such as Nup116p, Nup159p,Nsp1p, and Rip1p/Nup40p. These data suggest a model in whichnuclear mRNA export requires the Mex67p/Mtr2p heterodimericcomplex to directly contact several repeat nucleoporins, organizedin different nuclear pore complex subcomplexes, as it carriesthe mRNP cargo through the nuclear pore.

Key Words: nucleocytoplasmic transport • Nup116 • Nup159 • Rip1 • nuclear pore complex

Abbreviations used in this paper: C domain, COOH-terminal domain;CTE, constitutive transport element; hc, high-copy; LMB, leptomycinB; LRR domain, leucine-rich repeat domain; M domain, middledomain; N domain, NH₂-terminal domain; NES, nuclear export activity;NPC, nuclear pore complex; Nups, nucleoporins; ORF, open readingframe; ts, temperature-sensitive.

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Macara, I. G. (2001). Transport into and out of the Nucleus. Microbiol. Mol. Biol. Rev. 65: 570-594 [Abstract] [Full Text]
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Grosshans, H., Deinert, K., Hurt, E., Simos, G. (2001). Biogenesis of the Signal Recognition Particle (Srp) Involves Import of Srp Proteins into the Nucleolus, Assembly with the Srp-Rna, and Xpo1p-Mediated Export. JCB 153: 745-762 [Abstract] [Full Text]
Schisa, J., Pitt, J., Priess, J. (2001). Analysis of RNA associated with P granules in germ cells of C. elegans adults. Development 128: 1287-1298 [Abstract]
Herold, A., Suyama, M., Rodrigues, J. P., Braun, I. C., Kutay, U., Carmo-Fonseca, M., Bork, P., Izaurralde, E. (2000). TAP (NXF1) Belongs to a Multigene Family of Putative RNA Export Factors with a Conserved Modular Architecture. Mol. Cell. Biol. 20: 8996-9008 [Abstract] [Full Text]
Strawn, L. A., Shen, T., Wente, S. R. (2001). The GLFG Regions of Nup116p and Nup100p Serve as Binding Sites for Both Kap95p and Mex67p at the Nuclear Pore Complex. J. Biol. Chem. 276: 6445-6452 [Abstract] [Full Text]
Braun, I. C., Herold, A., Rode, M., Conti, E., Izaurralde, E. (2001). Overexpression of TAP/p15 Heterodimers Bypasses Nuclear Retention and Stimulates Nuclear mRNA Export. J. Biol. Chem. 276: 20536-20543 [Abstract] [Full Text]
Allen, N. P. C., Huang, L., Burlingame, A., Rexach, M. (2001). Proteomic Analysis of Nucleoporin Interacting Proteins. J. Biol. Chem. 276: 29268-29274 [Abstract] [Full Text]
Levesque, L., Guzik, B., Guan, T., Coyle, J., Black, B. E., Rekosh, D., Hammarskjold, M.-L., Paschal, B. M. (2001). RNA Export Mediated by Tap Involves NXT1-dependent Interactions with the Nuclear Pore Complex. J. Biol. Chem. 276: 44953-44962 [Abstract] [Full Text]
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