Peroxisomal Membrane Fusion Requires Two AAA Family ATPases, Pex1p and Pex6p

doi:10.1083/jcb.150.4.881

Published online 21 August 2000. doi:10.1083/jcb.150.4.881

This Article

	Full Text
	Full Text (PDF, 161K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Titorenko, V. I.
	Articles by Rachubinski, R. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Titorenko, V. I.
	Articles by Rachubinski, R. A.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/2000/8/881/ $5.00
The Journal of Cell Biology, Volume 150, Number 4, August 21, 2000 881-886

Report

Peroxisomal Membrane Fusion Requires Two AAA Family ATPases, Pex1p and Pex6p

Vladimir I. Titorenko^a and Richard A. Rachubinski^a
^a Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada

Correspondence to: Richard A. Rachubinski, Department of Cell Biology, University of Alberta, Medical Sciences Building 5-14, Edmonton, Alberta T6G 2H7, Canada. Tel:(780) 492-9868 Fax:(780) 492-9278 E-mail:rick.rachubinski{at}ualberta.ca.

Two AAA family ATPases, NSF and p97, have been implicated inmembrane fusion during assembly and inheritance of organellesof the secretory pathway. We have now investigated the rolesof AAA ATPases in membrane fusion during assembly of the peroxisome,an organelle outside the classical secretory system. Here, weshow that peroxisomal membrane fusion in the yeast Yarrowialipolytica requires two AAA ATPases, Pex1p and Pex6p. Releaseof membrane- associated Pex1p and Pex6p drives the asymmetricpriming of two fusion partners. The next step, peroxisome docking,requires release of Pex1p from one partner. Subsequent fusionof the peroxisomal membranes is independent of both Pex1p andPex6p.

Key Words: microbody, biogenesis, organelle assembly, in vitro reconstitution,peroxin

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Karnik, S. K., Trelease, R. N. (2007). Arabidopsis peroxin 16 trafficks through the ER and an intermediate compartment to pre-existing peroxisomes via overlapping molecular targeting signals. J Exp Bot 58: 1677-1693 [Abstract] [Full Text]
Idnurm, A., Giles, S. S., Perfect, J. R., Heitman, J. (2007). Peroxisome Function Regulates Growth on Glucose in the Basidiomycete Fungus Cryptococcus neoformans. Eukaryot Cell 6: 60-72 [Abstract] [Full Text]
Tamura, S., Yasutake, S., Matsumoto, N., Fujiki, Y. (2006). Dynamic and Functional Assembly of the AAA Peroxins, Pex1p and Pex6p, and Their Membrane Receptor Pex26p. J. Biol. Chem. 281: 27693-27704 [Abstract] [Full Text]
Titorenko, V. I., Mullen, R. T. (2006). Peroxisome biogenesis: the peroxisomal endomembrane system and the role of the ER. JCB 174: 11-17 [Abstract] [Full Text]
Wickner, W., Schekman, R. (2005). Protein Translocation Across Biological Membranes. Science 310: 1452-1456 [Abstract] [Full Text]
Karnik, S. K., Trelease, R. N. (2005). Arabidopsis Peroxin 16 Coexists at Steady State in Peroxisomes and Endoplasmic Reticulum. Plant Physiol. 138: 1967-1981 [Abstract] [Full Text]
Boukh-Viner, T., Guo, T., Alexandrian, A., Cerracchio, A., Gregg, C., Haile, S., Kyskan, R., Milijevic, S., Oren, D., Solomon, J., Wong, V., Nicaud, J.-M., Rachubinski, R. A., English, A. M., Titorenko, V. I. (2005). Dynamic ergosterol- and ceramide-rich domains in the peroxisomal membrane serve as an organizing platform for peroxisome fusion. JCB 168: 761-773 [Abstract] [Full Text]
Marelli, M., Smith, J. J., Jung, S., Yi, E., Nesvizhskii, A. I., Christmas, R. H., Saleem, R. A., Tam, Y. Y. C., Fagarasanu, A., Goodlett, D. R., Aebersold, R., Rachubinski, R. A., Aitchison, J. D. (2004). Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane. JCB 167: 1099-1112 [Abstract] [Full Text]
Shiozawa, K., Maita, N., Tomii, K., Seto, A., Goda, N., Akiyama, Y., Shimizu, T., Shirakawa, M., Hiroaki, H. (2004). Structure of the N-terminal Domain of PEX1 AAA-ATPase: CHARACTERIZATION OF A PUTATIVE ADAPTOR-BINDING DOMAIN. J. Biol. Chem. 279: 50060-50068 [Abstract] [Full Text]
Zolman, B. K., Bartel, B. (2004). An Arabidopsis indole-3-butyric acid-response mutant defective in PEROXIN6, an apparent ATPase implicated in peroxisomal function. Proc. Natl. Acad. Sci. USA 101: 1786-1791 [Abstract] [Full Text]
Birschmann, I., Stroobants, A. K., van den Berg, M., Schafer, A., Rosenkranz, K., Kunau, W.-H., Tabak, H. F. (2003). Pex15p of Saccharomyces cerevisiae Provides a Molecular Basis for Recruitment of the AAA Peroxin Pex6p to Peroxisomal Membranes. Mol. Biol. Cell 14: 2226-2236 [Abstract] [Full Text]
Bascom, R. A., Chan, H., Rachubinski, R. A. (2003). Peroxisome Biogenesis Occurs in an Unsynchronized Manner in Close Association with the Endoplasmic Reticulum in Temperature-sensitive Yarrowia lipolytica Pex3p Mutants. Mol. Biol. Cell 14: 939-957 [Abstract] [Full Text]
Harper, C. C., South, S. T., McCaffery, J. M., Gould, S. J. (2002). Peroxisomal Membrane Protein Import Does Not Require Pex17p. J. Biol. Chem. 277: 16498-16504 [Abstract] [Full Text]
Koenig, C., Araya, C., Skorin, C., Valencia, C., Toro, A., Leighton, F., Santos, M. J. (2002). Cytochemical and Biochemical Demonstration of an ATPase in Membranes of Human Peroxisomes. J. Histochem. Cytochem. 50: 405-414 [Abstract] [Full Text]
Hashiguchi, N., Kojidani, T., Imanaka, T., Haraguchi, T., Hiraoka, Y., Baumgart, E., Yokota, S., Tsukamoto, T., Osumi, T. (2002). Peroxisomes Are Formed from Complex Membrane Structures in PEX6-deficient CHO Cells upon Genetic Complementation. Mol. Biol. Cell 13: 711-722 [Abstract] [Full Text]