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Published online 21 August 2000. doi:10.1083/jcb.150.4.881
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© The Rockefeller University Press, 0021-9525/2000//881 $5.00
The Journal of Cell Biology, Volume 150, Number 4, , 2000 881-886

Report

Peroxisomal Membrane Fusion Requires Two Aaa Family Atpases, Pex1p and Pex6p



Vladimir I. Titorenkoa and Richard A. Rachubinskia

a Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
Department of Cell Biology, University of Alberta, Medical Sciences Building 5-14, Edmonton, Alberta T6G 2H7, Canada.(780) 492-9278(780) 492-9868

rick.rachubinski{at}ualberta.ca

Two AAA family ATPases, NSF and p97, have been implicated in membrane fusion during assembly and inheritance of organelles of the secretory pathway. We have now investigated the roles of AAA ATPases in membrane fusion during assembly of the peroxisome, an organelle outside the classical secretory system. Here, we show that peroxisomal membrane fusion in the yeast Yarrowia lipolytica requires two AAA ATPases, Pex1p and Pex6p. Release of membrane- associated Pex1p and Pex6p drives the asymmetric priming of two fusion partners. The next step, peroxisome docking, requires release of Pex1p from one partner. Subsequent fusion of the peroxisomal membranes is independent of both Pex1p and Pex6p.

Key Words: microbody • biogenesis • organelle assembly • in vitro reconstitution • peroxin

© 2000 The Rockefeller University Press

Abbreviations used in this paper: 20KgP, 20,000-g pellet; 20KgS, 20,000-g supernatant; 200KgP, 200,000-g pellet; 200KgS, 200,000-g supernatant (cytosol); mTHI, mature form of THI; NSF, N-ethylmaleimide–sensitive factor; PNS, postnuclear supernatant; pTHI, precursor form of THI; SNAP, soluble NSF attachment protein; SNARE, SNAP receptor; THI, thiolase.


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