Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 614K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Babiychuk, E. B. Articles by Draeger, A. Search for Related Content PubMed PubMed Citation Articles by Babiychuk, E. B. Articles by Draeger, A. Social Bookmarking                            What's this?

© The Rockefeller University Press, 0021-9525/2000//1113 $5.00
The Journal of Cell Biology, Volume 150, Number 5, , 2000 1113-1124

Annexins in Cell Membrane Dynamics

Eduard B. Babiychuk^a,b and Annette Draeger^a

^a Institute of Anatomy, University of Bern, 3012 Bern, Switzerland
^b Institute of Physiology, Kiev University, 252031 Kiev, Ukraine
Department of Cell Biology, Institute of Anatomy, University of Bern, Bühlstrasse 26, CH - 3012 Bern, Switzerland.41-31-631-38-0741-31-631-46-25

draeger{at}ana.unibe.ch

The sarcolemma of smooth muscle cells is composed of alternating stiff actin-binding, and flexible caveolar domains. In addition to these stable macrodomains, the plasma membrane contains dynamic glycosphingolipid- and cholesterol-enriched microdomains, which act as sorting posts for specific proteins and are involved in membrane trafficking and signal transduction. We demonstrate that these lipid rafts are neither periodically organized nor exclusively confined to the actin attachment sites or caveolar regions. Changes in the Ca²⁺ concentration that are affected during smooth muscle contraction lead to important structural rearrangements within the sarcolemma, which can be attributed to members of the annexin protein family. We show that the associations of annexins II, V, and VI with smooth muscle microsomal membranes exhibit a high degree of Ca²⁺ sensitivity, and that the extraction of annexins II and VI by detergent is prevented by elevated Ca²⁺ concentrations. Annexin VI participates in the formation of a reversible, membrane–cytoskeleton complex (Babiychuk, E.B., R.J. Palstra, J. Schaller, U. Kämpfer, and A. Draeger. 1999. J. Biol. Chem. 274:35191–35195). Annexin II promotes the Ca²⁺-dependent association of lipid raft microdomains, whereas annexin V interacts with glycerophospholipid microcompartments. These interactions bring about a new configuration of membrane-bound constituents, with potentially important consequences for signaling events and Ca²⁺ flux.

Key Words: DIGs • smooth muscle • rafts • caveolae • contraction

© 2000 The Rockefeller University Press

CiteULike

Complore

Connotea

Del.icio.us

Digg

Facebook

Reddit

Technorati

Twitter

This article has been cited by other articles:

• Wang, C.-Y., Lin, Y.-S., Su, W.-C., Chen, C.-L., Lin, C.-F. (2009). Glycogen Synthase Kinase-3 and Omi/HtrA2 Induce Annexin A2 Cleavage followed by Cell Cycle Inhibition and Apoptosis. Mol. Biol. Cell 20: 4153-4161 [Abstract] [Full Text]  
• Konopka-Postupolska, D., Clark, G., Goch, G., Debski, J., Floras, K., Cantero, A., Fijolek, B., Roux, S., Hennig, J. (2009). The Role of Annexin 1 in Drought Stress in Arabidopsis. Plant Physiol. 150: 1394-1410 [Abstract] [Full Text]  
• Monastyrskaya, K., Babiychuk, E. B., Hostettler, A., Wood, P., Grewal, T., Draeger, A. (2009). Plasma Membrane-associated Annexin A6 Reduces Ca2+ Entry by Stabilizing the Cortical Actin Cytoskeleton. J. Biol. Chem. 284: 17227-17242 [Abstract] [Full Text]  
• Hayes, M. J., Moss, S. E. (2009). Annexin 2 Has a Dual Role as Regulator and Effector of v-Src in Cell Transformation. J. Biol. Chem. 284: 10202-10210 [Abstract] [Full Text]  
• Cockrell, E, Espinola, R., McCrae, K. (2008). Annexin A2: biology and relevance to the antiphospholipid syndrome. Lupus 17: 944-952 [Abstract]  
• Heyraud, S., Jaquinod, M., Durmort, C., Dambroise, E., Concord, E., Schaal, J. P., Huber, P., Gulino-Debrac, D. (2008). Contribution of Annexin 2 to the Architecture of Mature Endothelial Adherens Junctions. Mol. Cell. Biol. 28: 1657-1668 [Abstract] [Full Text]  
• Mortimer, J. C., Laohavisit, A., Macpherson, N., Webb, A., Brownlee, C., Battey, N. H., Davies, J. M. (2008). Annexins: multifunctional components of growth and adaptation. J Exp Bot 0: erm344v1-erm344 [Abstract] [Full Text]  
• Genge, B. R., Wu, L. N. Y., Wuthier, R. E. (2007). In Vitro Modeling of Matrix Vesicle Nucleation: SYNERGISTIC STIMULATION OF MINERAL FORMATION BY ANNEXIN A5 AND PHOSPHATIDYLSERINE. J. Biol. Chem. 282: 26035-26045 [Abstract] [Full Text]  
• Zhang, Q., Wu, W. X. (2007). Separate and synergistic effect of progesterone and estradiol on induction of annexin 2 and its interaction protein p11 in pregnant sheep myometrium. J Mol Endocrinol 38: 441-454 [Abstract] [Full Text]  
• Chang, N., Sutherland, C., Hesse, E., Winkfein, R., Wiehler, W. B., Pho, M., Veillette, C., Li, S., Wilson, D. P., Kiss, E., Walsh, M. P. (2007). Identification of a novel interaction between the Ca2+-binding protein S100A11 and the Ca2+- and phospholipid-binding protein annexin A6. Am. J. Physiol. Cell Physiol. 292: C1417-C1430 [Abstract] [Full Text]  
• Fischer, T., Lu, L., Haigler, H. T., Langen, R. (2007). Annexin B12 Is a Sensor of Membrane Curvature and Undergoes Major Curvature-dependent Structural Changes. J. Biol. Chem. 282: 9996-10004 [Abstract] [Full Text]  
• Vijayasarathy, C., Takada, Y., Zeng, Y., Bush, R. A., Sieving, P. A. (2007). Retinoschisin Is a Peripheral Membrane Protein with Affinity for Anionic Phospholipids and Affected by Divalent Cations. IOVS 48: 991-1000 [Abstract] [Full Text]  
• Chintagari, N. R., Jin, N., Wang, P., Narasaraju, T. A., Chen, J., Liu, L. (2006). Effect of Cholesterol Depletion on Exocytosis of Alveolar Type II Cells. Am. J. Respir. Cell Mol. Bio. 34: 677-687 [Abstract] [Full Text]  
• Patschan, S., Li, H., Brodsky, S., Sullivan, D., De Angelis, D. A., Patschan, D., Goligorsky, M. S. (2006). Probing lipid rafts with proximity imaging: actions of proatherogenic stimuli. Am. J. Physiol. Heart Circ. Physiol. 290: H2210-H2219 [Abstract] [Full Text]  
• Burlak, C., Whitney, A. R., Mead, D. J., Hackstadt, T., DeLeo, F. R. (2006). Maturation of Human Neutrophil Phagosomes Includes Incorporation of Molecular Chaperones and Endoplasmic Reticulum Quality Control Machinery. Mol. Cell. Proteomics 5: 620-634 [Abstract] [Full Text]  
• Gokhale, N. A., Abraham, A., Digman, M. A., Gratton, E., Cho, W. (2005). Phosphoinositide Specificity of and Mechanism of Lipid Domain Formation by Annexin A2-p11 Heterotetramer. J. Biol. Chem. 280: 42831-42840 [Abstract] [Full Text]  
• Smith, R. D., Babiychuk, E. B., Noble, K., Draeger, A., Wray, S. (2005). Increased cholesterol decreases uterine activity: functional effects of cholesterol alteration in pregnant rat myometrium. Am. J. Physiol. Cell Physiol. 288: C982-C988 [Abstract] [Full Text]  
• Camors, E., Monceau, V., Charlemagne, D. (2005). Annexins and Ca2+ handling in the heart. Cardiovasc Res 65: 793-802 [Abstract] [Full Text]  
• Chasserot-Golaz, S., Vitale, N., Umbrecht-Jenck, E., Knight, D., Gerke, V., Bader, M.-F. (2005). Annexin 2 Promotes the Formation of Lipid Microdomains Required for Calcium-regulated Exocytosis of Dense-Core Vesicles. Mol. Biol. Cell 16: 1108-1119 [Abstract] [Full Text]  
• Monceau, V., Belikova, Y., Kratassiouk, G., Charue, D., Camors, E., Communal, C., Trouve, P., Russo-Marie, F., Charlemagne, D. (2004). Externalization of endogenous annexin A5 participates in apoptosis of rat cardiomyocytes. Cardiovasc Res 64: 496-506 [Abstract] [Full Text]  
• Riquelme, G., Llanos, P., Tischner, E., Neil, J., Campos, B. (2004). Annexin 6 Modulates the Maxi-chloride Channel of the Apical Membrane of Syncytiotrophoblast Isolated from Human Placenta. J. Biol. Chem. 279: 50601-50608 [Abstract] [Full Text]  
• Lee, D. B. N., Jamgotchian, N., Allen, S. G., Kan, F. W. K., Hale, I. L. (2004). Annexin A2 heterotetramer: role in tight junction assembly. Am. J. Physiol. Renal Physiol. 287: F481-F491 [Abstract] [Full Text]  
• Rescher, U., Ruhe, D., Ludwig, C., Zobiack, N., Gerke, V. (2004). Annexin 2 is a phosphatidylinositol (4,5)-bisphosphate binding protein recruited to actin assembly sites at cellular membranes. J. Cell Sci. 117: 3473-3480 [Abstract] [Full Text]  
• Skop, A. R., Liu, H., Yates, J. III, Meyer, B. J., Heald, R. (2004). Dissection of the Mammalian Midbody Proteome Reveals Conserved Cytokinesis Mechanisms. Science 305: 61-66 [Abstract] [Full Text]  
• Vruchte, D. t., Lloyd-Evans, E., Veldman, R. J., Neville, D. C. A., Dwek, R. A., Platt, F. M., van Blitterswijk, W. J., Sillence, D. J. (2004). Accumulation of Glycosphingolipids in Niemann-Pick C Disease Disrupts Endosomal Transport. J. Biol. Chem. 279: 26167-26175 [Abstract] [Full Text]  
• Rescher, U., Gerke, V. (2004). Annexins - unique membrane binding proteins with diverse functions. J. Cell Sci. 117: 2631-2639 [Abstract] [Full Text]  
• Filipenko, N. R., MacLeod, T. J., Yoon, C.-S., Waisman, D. M. (2004). Annexin A2 Is a Novel RNA-binding Protein. J. Biol. Chem. 279: 8723-8731 [Abstract] [Full Text]  
• Gillette, J. M., Nielsen-Preiss, S. M. (2004). The role of annexin 2 in osteoblastic mineralization. J. Cell Sci. 117: 441-449 [Abstract] [Full Text]  
• Benaud, C., Gentil, B. J., Assard, N., Court, M., Garin, J., Delphin, C., Baudier, J. (2004). AHNAK interaction with the annexin 2/S100A10 complex regulates cell membrane cytoarchitecture. JCB 164: 133-144 [Abstract] [Full Text]  
• Lennon, N. J., Kho, A., Bacskai, B. J., Perlmutter, S. L., Hyman, B. T., Brown, R. H. Jr. (2003). Dysferlin Interacts with Annexins A1 and A2 and Mediates Sarcolemmal Wound-healing. J. Biol. Chem. 278: 50466-50473 [Abstract] [Full Text]  
• Zobiack, N., Rescher, U., Ludwig, C., Zeuschner, D., Gerke, V. (2003). The Annexin 2/S100A10 Complex Controls the Distribution of Transferrin Receptor-containing Recycling Endosomes. Mol. Biol. Cell 14: 4896-4908 [Abstract] [Full Text]  
• Chattopadhyay, S., Sun, P., Wang, P., Abonyo, B., Cross, N. L., Liu, L. (2003). Fusion of Lamellar Body with Plasma Membrane Is Driven by the Dual Action of Annexin II Tetramer and Arachidonic Acid. J. Biol. Chem. 278: 39675-39683 [Abstract] [Full Text]  
• Koltzscher, M., Neumann, C., Konig, S., Gerke, V. (2003). Ca2+-dependent Binding and Activation of Dormant Ezrin by Dimeric S100P. Mol. Biol. Cell 14: 2372-2384 [Abstract] [Full Text]  
• Tomas, A., Moss, S. E. (2003). Calcium- and Cell Cycle-dependent Association of Annexin 11 with the Nuclear Envelope. J. Biol. Chem. 278: 20210-20216 [Abstract] [Full Text]  
• Burkart, A., Samii, B., Corvera, S., Shpetner, H. S. (2003). Regulation of the SHP-2 Tyrosine Phosphatase by a Novel Cholesterol- and Cell Confluence-dependent Mechanism. J. Biol. Chem. 278: 18360-18367 [Abstract] [Full Text]  
• Harris, T. J. C., Ravandi, A., Awrey, D. E., Siu, C.-H. (2003). Cytoskeleton Interactions Involved in the Assembly and Function of Glycoprotein-80 Adhesion Complexes in Dictyostelium. J. Biol. Chem. 278: 2614-2623 [Abstract] [Full Text]  
• Roberts, M. S., Woods, A. J., Shaw, P. E., Norman, J. C. (2003). ERK1 Associates with alpha vbeta 3 Integrin and Regulates Cell Spreading on Vitronectin. J. Biol. Chem. 278: 1975-1985 [Abstract] [Full Text]  
• Thomas, D. D. H., Kaspar, K. M., Taft, W. B., Weng, N., Rodenkirch, L. A., Groblewski, G. E. (2002). Identification of Annexin VI as a Ca2+-sensitive CRHSP-28-binding Protein in Pancreatic Acinar Cells. J. Biol. Chem. 277: 35496-35502 [Abstract] [Full Text]  
• de Diego, I., Schwartz, F., Siegfried, H., Dauterstedt, P., Heeren, J., Beisiegel, U., Enrich, C., Grewal, T. (2002). Cholesterol Modulates the Membrane Binding and Intracellular Distribution of Annexin 6. J. Biol. Chem. 277: 32187-32194 [Abstract] [Full Text]  
• BABIYCHUK, E. B., MONASTYRSKAYA, K., BURKHARD, F. C., WRAY, S., DRAEGER, A. (2002). Modulating signaling events in smooth muscle: cleavage of annexin 2 abolishes its binding to lipid rafts. FASEB J. 16: 1177-1184 [Abstract] [Full Text]  
• Goligorsky, M. S., Li, H., Brodsky, S., Chen, J. (2002). Relationships between caveolae and eNOS: everything in proximity and the proximity of everything. Am. J. Physiol. Renal Physiol. 283: F1-F10 [Abstract] [Full Text]  
• Kunzelmann-Marche, C., Freyssinet, J.-M., Martinez, M. C. (2002). Loss of Plasma Membrane Phospholipid Asymmetry Requires Raft Integrity. ROLE OF TRANSIENT RECEPTOR POTENTIAL CHANNELS AND ERK PATHWAY. J. Biol. Chem. 277: 19876-19881 [Abstract] [Full Text]  
• Gerke, V., Moss, S. E. (2002). Annexins: From Structure to Function. Physiol. Rev. 82: 331-371 [Abstract] [Full Text]  
• Salzer, U., Hinterdorfer, P., Hunger, U., Borken, C., Prohaska, R. (2002). Ca++-dependent vesicle release from erythrocytes involves stomatin-specific lipid rafts, synexin (annexin VII), and sorcin. Blood 99: 2569-2577 [Abstract] [Full Text]  
• Zobiack, N., Rescher, U., Laarmann, S., Michgehl, S., Schmidt, M. A., Gerke, V. (2002). Cell-surface attachment of pedestal-forming enteropathogenic E. coli induces a clustering of raft components and a recruitment of annexin 2. J. Cell Sci. 115: 91-98 [Abstract] [Full Text]  
• Deora, A., Ratner, L. (2001). Viral Protein U (Vpu)-Mediated Enhancement of Human Immunodeficiency Virus Type 1 Particle Release Depends on the Rate of Cellular Proliferation. J. Virol. 75: 6714-6718 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents