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Published online 4 September 2000. doi:10.1083/jcb.150.5.949
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© The Rockefeller University Press, 0021-9525/2000//949 $5.00
The Journal of Cell Biology, Volume 150, Number 5, , 2000 949-962

Original Article

The ER Repeat Protein Yt521-B Localizes to a Novel Subnuclear Compartment



Oliver Naylera,b,c, Annette M. Hartmanna, and Stefan Stamma

a Max-Planck-Institute of Neurobiology, Max-Planck-Institute of Biochemistry, D-82152 Martinsried, Germany
b Department of Molecular Biology, Max-Planck-Institute of Biochemistry, D-82152 Martinsried, Germany
c Actelion Ltd., CH-4123 Allschwil, Switzerland
Max-Planck Institute of Neurobiology, Am Klopferspitz 18a, D-82152 Martinsried, Germany.49 89 8578 374949 89 8578 3625

stefan{at}stamms-lab.net

The characterization of distinct subnuclear domains suggests a dynamic nuclear framework supporting gene expression and DNA replication. Here, we show that the glutamic acid/arginine-rich domain protein YT521-B localizes to a novel subnuclear structure, the YT bodies. YT bodies are dynamic compartments, which first appear at the beginning of S-phase in the cell cycle and disperse during mitosis. Furthermore, in untreated cells of the human cell line MCF7 they were undetectable and appeared only after drug- induced differentiation. YT bodies contain transcriptionally active sites and are in close contact to other subnuclear structures such as speckles and coiled bodies. YT bodies disperse upon actinomycin D treatment, whereas other transcriptional inhibitors such as {alpha}-amanitin or DRB have little effect. On the basis of our experiments, we propose that YT521-B may participate in the assembly of genes into transcription centers, thereby allowing efficient regulation of gene expression.

Key Words: subnuclear compartments • transcription • cell cycle • MCF7 differentiation • actinomycin D

© 2000 The Rockefeller University Press

Abbreviations used in this paper: DRB, 5,6-dichloro-1-β-ribofuranosyl-benzimidazole; EGFP, enhanced green fluorescent protein; HNTG, Hepes/NaCl/Triton X-100/Glycerol; OPT, Oct1/PTF/transcription domain; PML, promyelocytic leukemia protein; PP1, [4-amino-5-(4-methylphenyl)-7-(t-butyl) pyrazolo [3,4-d]-pyrimidine; RIPA, radioimmune precipitation assay; SAF-B, scaffold attachment factor B; SAR/MAR region, scaffold attachment/matrix attachment region; SR protein, serine/arginine-rich protein.


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