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Published online 16 October 2000. doi:10.1083/jcb.151.2.439
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© The Rockefeller University Press, 0021-9525/2000//439 $5.00
The Journal of Cell Biology, Volume 151, Number 2, , 2000 439-452

Original Article

Ordering the Final Events in Yeast Exocytosis



Eric Grotea, Chavela M. Carra, and Peter J. Novicka

a Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520
Dept. Cell Biology, Yale University School of Medicine, 333 Cedar St., New Haven, CT 06520-8002.(203) 785-7226(203) 785-5871

In yeast, assembly of exocytic soluble N-ethylmaleimide–sensitive fusion protein (NSF) attachment protein receptor (SNARE) complexes between the secretory vesicle SNARE Sncp and the plasma membrane SNAREs Ssop and Sec9p occurs at a late stage of the exocytic reaction. Mutations that block either secretory vesicle delivery or tethering prevent SNARE complex assembly and the localization of Sec1p, a SNARE complex binding protein, to sites of secretion. By contrast, wild-type levels of SNARE complexes persist in the sec1-1 mutant after a secretory block is imposed, suggesting a role for Sec1p after SNARE complex assembly. In the sec18-1 mutant, cis-SNARE complexes containing surface-accessible Sncp accumulate in the plasma membrane. Thus, one function of Sec18p is to disassemble SNARE complexes on the postfusion membrane.

Key Words: NSF • membrane fusion • SNAREs • exocyst • Sec1

© 2000 The Rockefeller University Press

Abbreviations used in this paper: GFP, green fluorescent protein; HA, hemagglutinin; NSF, N-ethylmaleimide–sensitive fusion protein; SNARE, soluble NSF attachment protein receptor.


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