Ordering the Final Events in Yeast Exocytosis

doi:10.1083/jcb.151.2.439

Published online 18 October 2000. doi:10.1083/jcb.151.2.439

This Article

	Full Text
	Full Text (PDF, 438K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Grote, E.
	Articles by Novick, P. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Grote, E.
	Articles by Novick, P. J.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/2000/10/439/ $5.00
The Journal of Cell Biology, Volume 151, Number 2, October 16, 2000 439-452

Original Article

Ordering the Final Events in Yeast Exocytosis

Eric Grote^a, Chavela M. Carr^a, and Peter J. Novick^a
^a Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520

Correspondence to: Peter J. Novick, Dept. Cell Biology, Yale University School of Medicine, 333 Cedar St., New Haven, CT 06520-8002. Tel:(203) 785-5871 Fax:(203) 785-7226

In yeast, assembly of exocytic soluble N-ethylmaleimide–sensitivefusion protein (NSF) attachment protein receptor (SNARE) complexesbetween the secretory vesicle SNARE Sncp and the plasma membraneSNAREs Ssop and Sec9p occurs at a late stage of the exocyticreaction. Mutations that block either secretory vesicle deliveryor tethering prevent SNARE complex assembly and the localizationof Sec1p, a SNARE complex binding protein, to sites of secretion.By contrast, wild-type levels of SNARE complexes persist inthe sec1-1 mutant after a secretory block is imposed, suggestinga role for Sec1p after SNARE complex assembly. In the sec18-1mutant, cis-SNARE complexes containing surface-accessible Sncpaccumulate in the plasma membrane. Thus, one function of Sec18pis to disassemble SNARE complexes on the postfusion membrane.

Key Words: NSF, membrane fusion, SNAREs, exocyst, Sec1

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Hutagalung, A. H., Coleman, J., Pypaert, M., Novick, P. J. (2009). An Internal Domain of Exo70p Is Required for Actin-independent Localization and Mediates Assembly of Specific Exocyst Components. Mol. Biol. Cell 20: 153-163 [Abstract] [Full Text]
Schilde, C., Lutter, K., Kissmehl, R., Plattner, H. (2008). Molecular Identification of a SNAP-25-Like SNARE Protein in Paramecium. Eukaryot Cell 7: 1387-1402 [Abstract] [Full Text]
Kuner, T., Li, Y., Gee, K. R., Bonewald, L. F., Augustine, G. J. (2008). Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release. Proc. Natl. Acad. Sci. USA 105: 347-352 [Abstract] [Full Text]
Togneri, J., Cheng, Y.-S., Munson, M., Hughson, F. M., Carr, C. M. (2006). Specific SNARE complex binding mode of the Sec1/Munc-18 protein, Sec1p. Proc. Natl. Acad. Sci. USA 103: 17730-17735 [Abstract] [Full Text]
Chen, X., Arac, D., Wang, T.-M., Gilpin, C. J., Zimmerberg, J., Rizo, J. (2006). SNARE-Mediated Lipid Mixing Depends on the Physical State of the Vesicles. Biophys. J 90: 2062-2074 [Abstract] [Full Text]
Castillo-Flores, A., Weinberger, A., Robinson, M., Gerst, J. E. (2005). Mso1 Is a Novel Component of the Yeast Exocytic SNARE Complex. J. Biol. Chem. 280: 34033-34041 [Abstract] [Full Text]
Ciufo, L. F., Barclay, J. W., Burgoyne, R. D., Morgan, A. (2005). Munc18-1 Regulates Early and Late Stages of Exocytosis via Syntaxin-independent Protein Interactions. Mol. Biol. Cell 16: 470-482 [Abstract] [Full Text]
Wiederkehr, A., De Craene, J.-O., Ferro-Novick, S., Novick, P. (2004). Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. JCB 167: 875-887 [Abstract] [Full Text]
Scott, B. L., Van Komen, J. S., Irshad, H., Liu, S., Wilson, K. A., McNew, J. A. (2004). Sec1p directly stimulates SNARE-mediated membrane fusion in vitro. JCB 167: 75-85 [Abstract] [Full Text]
Graham, M. E., Barclay, J. W., Burgoyne, R. D. (2004). Syntaxin/Munc18 Interactions in the Late Events during Vesicle Fusion and Release in Exocytosis. J. Biol. Chem. 279: 32751-32760 [Abstract] [Full Text]
Murthy, M., Schwarz, T. L. (2004). The exocyst component Sec5 is required for membrane traffic and polarity in the Drosophila ovary. Development 131: 377-388 [Abstract] [Full Text]
Prigent, M., Dubois, T., Raposo, G., Derrien, V., Tenza, D., Rosse, C., Camonis, J., Chavrier, P. (2003). ARF6 controls post-endocytic recycling through its downstream exocyst complex effector. JCB 163: 1111-1121 [Abstract] [Full Text]
Wiederkehr, A., Du, Y., Pypaert, M., Ferro-Novick, S., Novick, P. (2003). Sec3p Is Needed for the Spatial Regulation of Secretion and for the Inheritance of the Cortical Endoplasmic Reticulum. Mol. Biol. Cell 14: 4770-4782 [Abstract] [Full Text]
Sesaki, H., Southard, S. M., Yaffe, M. P., Jensen, R. E. (2003). Mgm1p, a Dynamin-related GTPase, Is Essential for Fusion of the Mitochondrial Outer Membrane. Mol. Biol. Cell 14: 2342-2356 [Abstract] [Full Text]
Bryant, N. J., James, D. E. (2003). The Sec1p/Munc18 (SM) protein, Vps45p, cycles on and off membranes during vesicle transport. JCB 161: 691-696 [Abstract] [Full Text]
Houng, A., Polgar, J., Reed, G. L. (2003). Munc18-Syntaxin Complexes and Exocytosis in Human Platelets. J. Biol. Chem. 278: 19627-19633 [Abstract] [Full Text]
Kosodo, Y., Noda, Y., Adachi, H., Yoda, K. (2002). Binding of Sly1 to Sed5 enhances formation of the yeast early Golgi SNARE complex. J. Cell Sci. 115: 3683-3691 [Abstract] [Full Text]
Froissard, M., Kissmehl, R., Dedieu, J.-C., Gulik-Krzywicki, T., Plattner, H., Cohen, J. (2002). N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional Exocytotic Microdomains in Paramecium. Genetics 161: 643-650 [Abstract] [Full Text]
Peng, R., Gallwitz, D. (2002). Sly1 protein bound to Golgi syntaxin Sed5p allows assembly and contributes to specificity of SNARE fusion complexes. JCB 157: 645-655 [Abstract] [Full Text]
Munson, M., Hughson, F. M. (2002). Conformational Regulation of SNARE Assembly and Disassembly in Vivo. J. Biol. Chem. 277: 9375-9381 [Abstract] [Full Text]
Brickner, J. H., Blanchette, J. M., Sipos, G., Fuller, R. S. (2001). The Tlg SNARE complex is required for TGN homotypic fusion. JCB 155: 969-978 [Abstract] [Full Text]
Laage, R., Ungermann, C. (2001). The N-terminal Domain of the t-SNARE Vam3p Coordinates Priming and Docking in Yeast Vacuole Fusion. Mol. Biol. Cell 12: 3375-3385 [Abstract] [Full Text]
Littleton, J. T., Barnard, R. J. O., Titus, S. A., Slind, J., Chapman, E. R., Ganetzky, B. (2001). SNARE-complex disassembly by NSF follows synaptic-vesicle fusion. Proc. Natl. Acad. Sci. USA 10.1073/pnas.221450198v1 [Abstract] [Full Text]
Fisher, R. J., Pevsner, J., Burgoyne, R. D. (2001). Control of Fusion Pore Dynamics During Exocytosis by Munc18. Science 291: 875-878 [Abstract] [Full Text]
Grote, E., Vlacich, G., Pypaert, M., Novick, P. J. (2000). A snc1 Endocytosis Mutant: Phenotypic Analysis and Suppression by Overproduction of Dihydrosphingosine Phosphate Lyase. Mol. Biol. Cell 11: 4051-4065 [Abstract] [Full Text]
Grote, E., Baba, M., Ohsumi, Y., Novick, P. J. (2000). Geranylgeranylated SNAREs Are Dominant Inhibitors of Membrane Fusion. JCB 151: 453-466 [Abstract] [Full Text]
Littleton, J. T., Barnard, R. J. O., Titus, S. A., Slind, J., Chapman, E. R., Ganetzky, B. (2001). SNARE-complex disassembly by NSF follows synaptic-vesicle fusion. Proc. Natl. Acad. Sci. USA 98: 12233-12238 [Abstract] [Full Text]