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Published online 30 October 2000. doi:10.1083/jcb.151.3.587
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© The Rockefeller University Press, 0021-9525/2000//587 $5.00
The Journal of Cell Biology, Volume 151, Number 3, , 2000 587-600

Original Article

A Selective Transport Route from Golgi to Late Endosomes That Requires the Yeast Gga Proteins



Michael W. Blacka and Hugh R.B. Pelhama

a Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.44-1223-41214244-1223-402290

Pep12p is a yeast syntaxin located primarily in late endosomes. Using mutagenesis of a green fluorescent protein chimera we have identified a sorting signal FSDSPEF, which is required for transport of Pep12p from the exocytic pathway to late endosomes, from which it can, when overexpressed, reach the vacuole. When this signal is mutated, Pep12p instead passes to early endosomes, a step that is determined by its transmembrane domain. Surprisingly, Pep12p is then specifically retained in early endosomes and does not go on to late endosomes.

By testing appropriate chimeras in mutant strains, we found that FSDSPEF-dependent sorting was abolished in strains lacking Gga1p and Gga2p, Golgi-associated coat proteins with homology to gamma adaptin. In the gga1 gga2 double mutant endogenous Pep12p cofractionated with the early endosome marker Tlg1p, and recycling of Snc1p through early endosomes was defective. Pep12p sorting was also defective in cells lacking the clathrin heavy or light chain. We suggest that specific and direct delivery of proteins to early and late endosomes is required to maintain the functional heterogeneity of the endocytic pathway and that the GGA proteins, probably in association with clathrin, help create vesicles destined for late endosomes.

Key Words: clathrin • endosomes • GGA proteins • Golgi apparatus • Pep12p

© 2000 The Rockefeller University Press

Abbreviations used in this paper: CPY, carboxypeptidase Y; GFP, green fluorescent protein; PVC, pre-vacuolar compartment; SNARE, soluble N-ethylmaleimide–sensitive fusion protein attachment protein receptor; TMD, transmembrane domain.


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