Pds5p Is an Essential Chromosomal Protein Required for Both Sister Chromatid Cohesion and Condensation in Saccharomyces cerevisiae

doi:10.1083/jcb.151.3.613

Published online 30 October 2000. doi:10.1083/jcb.151.3.613

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© The Rockefeller University Press, 0021-9525/2000//613 $5.00
The Journal of Cell Biology, Volume 151, Number 3, , 2000 613-626

Original Article

Pds5p Is an Essential Chromosomal Protein Required for Both Sister Chromatid Cohesion and Condensation in Saccharomyces cerevisiae

Theresa Hartman^a, Kristen Stead^a, Douglas Koshland^b, and Vincent Guacci^a

^a Fox Chase Cancer Center, Basic Science Division, Philadelphia, Pennsylvania 19111
^b Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210
Fox Chase Cancer Center, Basic Science Division, Room W462, 7701 Burholme Avenue, Philadelphia, PA 19111.(215) 728-3616(215) 728-5632

The PDS5 gene (precocious dissociation of sisters) was identifiedin a genetic screen designed to identify genes important forchromosome structure. PDS5 is an essential gene and homologuesare found from yeast to humans. Pds5p function is importantfor viability from S phase through mitosis and localizes tochromosomes during this cell cycle window, which encompassesthe times when sister chromatid cohesion exists. Pds5p is requiredto maintain cohesion at centromere proximal and distal sequences.These properties are identical to those of the four cohesioncomplex members Mcd1p/Scc1p, Smc1p, Smc3p, and Scc3p/Irr1p (Guacci,V., D. Koshland, and A. Strunnikov. 1997. Cell. 91:47–57;Michaelis, C., R. Ciosk, and K. Nasmyth. 1997. Cell. 91:35–45;Toth, A., R. Ciosk, F. Uhlmann, M. Galova, A. Schleiffer, andK. Nasmyth. 1999. Genes Dev. 13:307–319). Pds5p bindsto centromeric and arm sequences bound by Mcd1p. Furthermore,Pds5p localization to chromosomes is dependent on Mcd1p. Thus,Pds5p, like the cohesin complex members, is a component of themolecular glue that mediates sister chromatid cohesion. However,Mcd1p localization to chromosomes is independent of Pds5p, whichmay reflect differences in their roles in cohesion. Finally,Pds5p is required for condensation as well as cohesion, whichconfirms the link between these processes revealed through analysisof Mcd1p (Guacci, V., D. Koshland, and A. Strunnikov. 1997.Cell. 91:47–57). Therefore, the link between cohesionand condensation is a general property of yeast chromosomes.

Key Words: mitosis • chromosomes • cohesion • condensation • yeast

Abbreviations used in this paper: ChIP, chromatin immunoprecipitation;FISH, fluorescent in situ hybridization; GFP, green fluorescentprotein; HA, hemagglutinin.

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