Chlamydomonas IFT88 and Its Mouse Homologue, Polycystic Kidney Disease Gene Tg737, Are Required for Assembly of Cilia and Flagella

doi:10.1083/jcb.151.3.709

Published online 30 October 2000. doi:10.1083/jcb.151.3.709

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© The Rockefeller University Press, 0021-9525/2000/10/709/ $5.00
The Journal of Cell Biology, Volume 151, Number 3, October 30, 2000 709-718

Original Article

Chlamydomonas IFT88 and Its Mouse Homologue, Polycystic Kidney Disease Gene Tg737, Are Required for Assembly of Cilia and Flagella

Gregory J. Pazour^a, Bethany L. Dickert^a, Yvonne Vucica^b, E. Scott Seeley^b, Joel L. Rosenbaum^b, George B. Witman^a, and Douglas G. Cole^c
^a Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655
^b Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520
^c Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844

Correspondence to: Douglas G. Cole, Department of Microbiology, Molecular Biology, and Biochemistry, Life Science South 142, University of Idaho, Moscow, ID 83844-3052. Tel:208-885-4071 Fax:208-885-6518

Intraflagellar transport (IFT) is a rapid movement of multi-subunitprotein particles along flagellar microtubules and is requiredfor assembly and maintenance of eukaryotic flagella. We clonedand sequenced a Chlamydomonas cDNA encoding the IFT88 subunitof the IFT particle and identified a Chlamydomonas insertionalmutant that is missing this gene. The phenotype of this mutantis normal except for the complete absence of flagella. IFT88is homologous to mouse and human genes called Tg737. Mice withdefects in Tg737 die shortly after birth from polycystic kidneydisease. We show that the primary cilia in the kidney of Tg737mutant mice are shorter than normal. This indicates that IFTis important for primary cilia assembly in mammals. It is likelythat primary cilia have an important function in the kidneyand that defects in their assembly can lead to polycystic kidneydisease.

Key Words: orpk, intraflagellar transport, primary cilia, kinesin-II, cytoplasmicdynein

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Tsao, C.-C., Gorovsky, M. A. (2008). Different Effects of Tetrahymena IFT172 Domains on Anterograde and Retrograde Intraflagellar Transport. Mol. Biol. Cell 19: 1450-1461 [Abstract] [Full Text]
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Tsao, C.-C., Gorovsky, M. A. (2008). Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body. J. Cell Sci. 121: 428-436 [Abstract] [Full Text]
Lechtreck, K.-F., Delmotte, P., Robinson, M. L., Sanderson, M. J., Witman, G. B. (2008). Mutations in Hydin impair ciliary motility in mice. JCB 180: 633-643 [Abstract] [Full Text]
McClintock, T. S., Glasser, C. E., Bose, S. C., Bergman, D. A. (2008). Tissue expression patterns identify mouse cilia genes. Physiol. Genomics 32: 198-206 [Abstract] [Full Text]
Sloboda, R. D., Rosenbaum, J. L. (2007). Making sense of cilia and flagella. JCB 179: 575-582 [Abstract] [Full Text]
Huang, K., Diener, D. R., Mitchell, A., Pazour, G. J., Witman, G. B., Rosenbaum, J. L. (2007). Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella. JCB 179: 501-514 [Abstract] [Full Text]
Weimbs, T. (2007). Polycystic kidney disease and renal injury repair: common pathways, fluid flow, and the function of polycystin-1. Am. J. Physiol. Renal Physiol. 293: F1423-F1432 [Abstract] [Full Text]
Vladar, E. K., Stearns, T. (2007). Molecular characterization of centriole assembly in ciliated epithelial cells. JCB 178: 31-42 [Abstract] [Full Text]
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Vierkotten, J., Dildrop, R., Peters, T., Wang, B., Ruther, U. (2007). Ftm is a novel basal body protein of cilia involved in Shh signalling. Development 134: 2569-2577 [Abstract] [Full Text]
Koyama, E., Young, B., Nagayama, M., Shibukawa, Y., Enomoto-Iwamoto, M., Iwamoto, M., Maeda, Y., Lanske, B., Song, B., Serra, R., Pacifici, M. (2007). Conditional Kif3a ablation causes abnormal hedgehog signaling topography, growth plate dysfunction, and excessive bone and cartilage formation during mouse skeletogenesis. Development 134: 2159-2169 [Abstract] [Full Text]
Yoder, B. K. (2007). Role of Primary Cilia in the Pathogenesis of Polycystic Kidney Disease. J. Am. Soc. Nephrol. 18: 1381-1388 [Abstract] [Full Text]
Ait-Lounis, A., Baas, D., Barras, E., Benadiba, C., Charollais, A., Nlend Nlend, R., Liegeois, D., Meda, P., Durand, B., Reith, W. (2007). Novel Function of the Ciliogenic Transcription Factor RFX3 in Development of the Endocrine Pancreas. Diabetes 56: 950-959 [Abstract] [Full Text]
Banizs, B., Komlosi, P., Bevensee, M. O., Schwiebert, E. M., Bell, P. D., Yoder, B. K. (2007). Altered pHi regulation and Na+/HCO3- transporter activity in choroid plexus of cilia-defective Tg737orpk mutant mouse. Am. J. Physiol. Cell Physiol. 292: C1409-C1416 [Abstract] [Full Text]
Liu, Y., Pathak, N., Kramer-Zucker, A., Drummond, I. A. (2007). Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros. Development 134: 1111-1122 [Abstract] [Full Text]
Xu, C., Rossetti, S., Jiang, L., Harris, P. C., Brown-Glaberman, U., Wandinger-Ness, A., Bacallao, R., Alper, S. L. (2007). Human ADPKD primary cyst epithelial cells with a novel, single codon deletion in the PKD1 gene exhibit defective ciliary polycystin localization and loss of flow-induced Ca2+ signaling. Am. J. Physiol. Renal Physiol. 292: F930-F945 [Abstract] [Full Text]
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Robert, A., Margall-Ducos, G., Guidotti, J.-E., Bregerie, O., Celati, C., Brechot, C., Desdouets, C. (2007). The intraflagellar transport component IFT88/polaris is a centrosomal protein regulating G1-S transition in non-ciliated cells. J. Cell Sci. 120: 628-637 [Abstract] [Full Text]
Lechtreck, K.-F., Witman, G. B. (2007). Chlamydomonas reinhardtii hydin is a central pair protein required for flagellar motility. JCB 176: 473-482 [Abstract] [Full Text]
Hossain, Z., Ali, S. M., Ko, H. L., Xu, J., Ng, C. P., Guo, K., Qi, Z., Ponniah, S., Hong, W., Hunziker, W. (2007). Glomerulocystic kidney disease in mice with a targeted inactivation of Wwtr1. Proc. Natl. Acad. Sci. USA 104: 1631-1636 [Abstract] [Full Text]
Dawe, H. R., Smith, U. M., Cullinane, A. R., Gerrelli, D., Cox, P., Badano, J. L., Blair-Reid, S., Sriram, N., Katsanis, N., Attie-Bitach, T., Afford, S. C., Copp, A. J., Kelly, D. A., Gull, K., Johnson, C. A. (2007). The Meckel-Gruber Syndrome proteins MKS1 and meckelin interact and are required for primary cilium formation. Hum Mol Genet 16: 173-186 [Abstract] [Full Text]
Haycraft, C. J., Zhang, Q., Song, B., Jackson, W. S., Detloff, P. J., Serra, R., Yoder, B. K. (2007). Intraflagellar transport is essential for endochondral bone formation. Development 134: 307-316 [Abstract] [Full Text]
Dawe, H. R., Farr, H., Gull, K. (2007). Centriole/basal body morphogenesis and migration during ciliogenesis in animal cells. J. Cell Sci. 120: 7-15 [Abstract] [Full Text]
Rapoport, J. (2007). Autosomal dominant polycystic kidney disease: pathophysiology and treatment. QJM 100: 1-9 [Full Text]
Blacque, O. E., Li, C., Inglis, P. N., Esmail, M. A., Ou, G., Mah, A. K., Baillie, D. L., Scholey, J. M., Leroux, M. R. (2006). The WD Repeat-containing Protein IFTA-1 Is Required for Retrograde Intraflagellar Transport. Mol. Biol. Cell 17: 5053-5062 [Abstract] [Full Text]
Wu, Y., Dai, X.-Q., Li, Q., Chen, C. X., Mai, W., Hussain, Z., Long, W., Montalbetti, N., Li, G., Glynne, R., Wang, S., Cantiello, H. F., Wu, G., Chen, X.-Z. (2006). Kinesin-2 mediates physical and functional interactions between polycystin-2 and fibrocystin. Hum Mol Genet 15: 3280-3292 [Abstract] [Full Text]
Fliegauf, M., Horvath, J., von Schnakenburg, C., Olbrich, H., Muller, D., Thumfart, J., Schermer, B., Pazour, G. J., Neumann, H. P.H., Zentgraf, H., Benzing, T., Omran, H. (2006). Nephrocystin Specifically Localizes to the Transition Zone of Renal and Respiratory Cilia and Photoreceptor Connecting Cilia. J. Am. Soc. Nephrol. 17: 2424-2433 [Abstract] [Full Text]
Follit, J. A., Tuft, R. A., Fogarty, K. E., Pazour, G. J. (2006). The Intraflagellar Transport Protein IFT20 Is Associated with the Golgi Complex and Is Required for Cilia Assembly. Mol. Biol. Cell 17: 3781-3792 [Abstract] [Full Text]
Michaud, E. J., Yoder, B. K. (2006). The Primary Cilium in Cell Signaling and Cancer.. Cancer Res. 66: 6463-6467 [Abstract] [Full Text]
Escalier, D. (2006). Knockout mouse models of sperm flagellum anomalies. Hum Reprod Update 12: 449-461 [Abstract] [Full Text]
Esteban, M. A., Harten, S. K., Tran, M. G., Maxwell, P. H. (2006). Formation of Primary Cilia in the Renal Epithelium Is Regulated by the von Hippel-Lindau Tumor Suppressor Protein. J. Am. Soc. Nephrol. 17: 1801-1806 [Abstract] [Full Text]
Geng, L., Okuhara, D., Yu, Z., Tian, X., Cai, Y., Shibazaki, S., Somlo, S. (2006). Polycystin-2 traffics to cilia independently of polycystin-1 by using an N-terminal RVxP motif. J. Cell Sci. 119: 1383-1395 [Abstract] [Full Text]
Schrick, J. J., Vogel, P., Abuin, A., Hampton, B., Rice, D. S. (2006). ADP-Ribosylation Factor-Like 3 Is Involved in Kidney and Photoreceptor Development. Am. J. Pathol. 168: 1288-1298 [Abstract] [Full Text]
Zhang, Z., Kostetskii, I., Tang, W., Haig-Ladewig, L., Sapiro, R., Wei, Z., Patel, A. M., Bennett, J., Gerton, G. L., Moss, S. B., Radice, G. L., III, J. F. S. (2006). Deficiency of SPAG16L Causes Male Infertility Associated with Impaired Sperm Motility. Biol. Reprod. 74: 751-759 [Abstract] [Full Text]
Banizs, B., Pike, M. M., Millican, C. L., Ferguson, W. B., Komlosi, P., Sheetz, J., Bell, P. D., Schwiebert, E. M., Yoder, B. K. (2005). Dysfunctional cilia lead to altered ependyma and choroid plexus function, and result in the formation of hydrocephalus. Development 132: 5329-5339 [Abstract] [Full Text]
Davenport, J. R., Yoder, B. K. (2005). An incredible decade for the primary cilium: a look at a once-forgotten organelle. Am. J. Physiol. Renal Physiol. 289: F1159-F1169 [Abstract] [Full Text]
Winkelbauer, M. E., Schafer, J. C., Haycraft, C. J., Swoboda, P., Yoder, B. K. (2005). The C. elegans homologs of nephrocystin-1 and nephrocystin-4 are cilia transition zone proteins involved in chemosensory perception. J. Cell Sci. 118: 5575-5587 [Abstract] [Full Text]
Mahjoub, M. R., Trapp, M. L., Quarmby, L. M. (2005). NIMA-Related Kinases Defective in Murine Models of Polycystic Kidney Diseases Localize to Primary Cilia and Centrosomes. J. Am. Soc. Nephrol. 16: 3485-3489 [Abstract] [Full Text]
Liu, W., Murcia, N. S., Duan, Y., Weinbaum, S., Yoder, B. K., Schwiebert, E., Satlin, L. M. (2005). Mechanoregulation of intracellular Ca2+ concentration is attenuated in collecting duct of monocilium-impaired orpk mice. Am. J. Physiol. Renal Physiol. 289: F978-F988 [Abstract] [Full Text]
Louie, C. M., Gleeson, J. G. (2005). Genetic basis of Joubert syndrome and related disorders of cerebellar development. Hum Mol Genet 14: R235-R242 [Abstract] [Full Text]
Lucker, B. F., Behal, R. H., Qin, H., Siron, L. C., Taggart, W. D., Rosenbaum, J. L., Cole, D. G. (2005). Characterization of the Intraflagellar Transport Complex B Core: DIRECT INTERACTION OF THE IFT81 AND IFT74/72 SUBUNITS. J. Biol. Chem. 280: 27688-27696 [Abstract] [Full Text]
Quarmby, L. M., Parker, J. D.K. (2005). Cilia and the cell cycle?. JCB 169: 707-710 [Abstract] [Full Text]
Kramer-Zucker, A. G., Olale, F., Haycraft, C. J., Yoder, B. K., Schier, A. F., Drummond, I. A. (2005). Cilia-driven fluid flow in the zebrafish pronephros, brain and Kupffer's vesicle is required for normal organogenesis. Development 132: 1907-1921 [Abstract] [Full Text]
Mollet, G., Silbermann, F., Delous, M., Salomon, R., Antignac, C., Saunier, S. (2005). Characterization of the nephrocystin/nephrocystin-4 complex and subcellular localization of nephrocystin-4 to primary cilia and centrosomes. Hum Mol Genet 14: 645-656 [Abstract] [Full Text]
Drummond, I. A. (2005). Kidney Development and Disease in the Zebrafish. J. Am. Soc. Nephrol. 16: 299-304 [Abstract] [Full Text]
Barr, M. M. (2005). Caenorhabditis elegans as a Model to Study Renal Development and Disease: Sexy Cilia. J. Am. Soc. Nephrol. 16: 305-312 [Abstract] [Full Text]
Grossman, A. R. (2005). Paths toward Algal Genomics. Plant Physiol. 137: 410-427 [Full Text]
Masyuk, T. V., Huang, B. Q., Masyuk, A. I., Ritman, E. L., Torres, V. E., Wang, X., Harris, P. C., LaRusso, N. F. (2004). Biliary Dysgenesis in the PCK Rat, an Orthologous Model of Autosomal Recessive Polycystic Kidney Disease. Am. J. Pathol. 165: 1719-1730 [Abstract] [Full Text]
Pazour, G. J. (2004). Intraflagellar Transport and Cilia-Dependent Renal Disease: The Ciliary Hypothesis of Polycystic Kidney Disease. J. Am. Soc. Nephrol. 15: 2528-2536 [Abstract] [Full Text]
Romio, L., Fry, A. M., Winyard, P. J.D., Malcolm, S., Woolf, A. S., Feather, S. A. (2004). OFD1 Is a Centrosomal/Basal Body Protein Expressed during Mesenchymal-Epithelial Transition in Human Nephrogenesis. J. Am. Soc. Nephrol. 15: 2556-2568 [Abstract] [Full Text]
Hou, Y., Pazour, G. J., Witman, G. B. (2004). A Dynein Light Intermediate Chain, D1bLIC, Is Required for Retrograde Intraflagellar Transport. Mol. Biol. Cell 15: 4382-4394 [Abstract] [Full Text]
Jurczyk, A., Gromley, A., Redick, S., Agustin, J. S., Witman, G., Pazour, G. J., Peters, D. J.M., Doxsey, S. (2004). Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly. JCB 166: 637-643 [Abstract] [Full Text]
Sun, Z., Amsterdam, A., Pazour, G. J., Cole, D. G., Miller, M. S., Hopkins, N. (2004). A genetic screen in zebrafish identifies cilia genes as a principal cause of cystic kidney. Development 131: 4085-4093 [Abstract] [Full Text]
Zhang, Q., Taulman, P. D., Yoder, B. K. (2004). Cystic Kidney Diseases: All Roads Lead to the Cilium. Physiology 19: 225-230 [Abstract] [Full Text]
Baker, J. D., Adhikarakunnathu, S., Kernan, M. J. (2004). Mechanosensory-defective, male-sterile unc mutants identify a novel basal body protein required for ciliogenesis in Drosophila. Development 131: 3411-3422 [Abstract] [Full Text]
Cano, D. A., Murcia, N. S., Pazour, G. J., Hebrok, M. (2004). orpk mouse model of polycystic kidney disease reveals essential role of primary cilia in pancreatic tissue organization. Development 131: 3457-3467 [Abstract] [Full Text]
Rundle, D. R., Gorbsky, G., Tsiokas, L. (2004). PKD2 Interacts and Co-localizes with mDia1 to Mitotic Spindles of Dividing Cells: ROLE OF mDia1 IN PKD2 LOCALIZATION TO MITOTIC SPINDLES. J. Biol. Chem. 279: 29728-29739 [Abstract] [Full Text]
Blacque, O. E., Reardon, M. J., Li, C., McCarthy, J., Mahjoub, M. R., Ansley, S. J., Badano, J. L., Mah, A. K., Beales, P. L., Davidson, W. S., Johnsen, R. C., Audeh, M., Plasterk, R. H.A., Baillie, D. L., Katsanis, N., Quarmby, L. M., Wicks, S. R., Leroux, M. R. (2004). Loss of C. elegans BBS-7 and BBS-8 protein function results in cilia defects and compromised intraflagellar transport. Genes Dev. 18: 1630-1642 [Abstract] [Full Text]
Mykytyn, K., Mullins, R. F., Andrews, M., Chiang, A. P., Swiderski, R. E., Yang, B., Braun, T., Casavant, T., Stone, E. M., Sheffield, V. C. (2004). Bardet-Biedl syndrome type 4 (BBS4)-null mice implicate Bbs4 in flagella formation but not global cilia assembly. Proc. Natl. Acad. Sci. USA 101: 8664-8669 [Abstract] [Full Text]
Bonnafe, E., Touka, M., AitLounis, A., Baas, D., Barras, E., Ucla, C., Moreau, A., Flamant, F., Dubruille, R., Couble, P., Collignon, J., Durand, B., Reith, W. (2004). The Transcription Factor RFX3 Directs Nodal Cilium Development and Left-Right Asymmetry Specification. Mol. Cell. Biol. 24: 4417-4427 [Abstract] [Full Text]
You, Y., Huang, T., Richer, E. J., Schmidt, J.-E. H., Zabner, J., Borok, Z., Brody, S. L. (2004). Role of f-box factor foxj1 in differentiation of ciliated airway epithelial cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 286: L650-L657 [Abstract] [Full Text]
Wang, S., Luo, Y., Wilson, P. D., Witman, G. B., Zhou, J. (2004). The Autosomal Recessive Polycystic Kidney Disease Protein Is Localized to Primary Cilia, with Concentration in the Basal Body Area. J. Am. Soc. Nephrol. 15: 592-602 [Abstract] [Full Text]
Zhang, M.-Z., Mai, W., Li, C., Cho, S.-y., Hao, C., Moeckel, G., Zhao, R., Kim, I., Wang, J., Xiong, H., Wang, H., Sato, Y., Wu, Y., Nakanuma, Y., Lilova, M., Pei, Y., Harris, R. C., Li, S., Coffey, R. J., Sun, L., Wu, D., Chen, X.-Z., Breyer, M. D., Zhao, Z. J., McKanna, J. A., Wu, G. (2004). PKHD1 protein encoded by the gene for autosomal recessive polycystic kidney disease associates with basal bodies and primary cilia in renal epithelial cells. Proc. Natl. Acad. Sci. USA 101: 2311-2316 [Abstract] [Full Text]
Qin, H., Diener, D. R., Geimer, S., Cole, D. G., Rosenbaum, J. L. (2004). Intraflagellar transport (IFT) cargo: IFT transports flagellar precursors to the tip and turnover products to the cell body. JCB 164: 255-266 [Abstract] [Full Text]
Tam, L.-W., Dentler, W. L., Lefebvre, P. A. (2003). Defective flagellar assembly and length regulation in LF3 null mutants in Chlamydomonas. JCB 163: 597-607 [Abstract] [Full Text]
Thomson, R. B., Mentone, S., Kim, R., Earle, K., Delpire, E., Somlo, S., Aronson, P. S. (2003). Histopathological analysis of renal cystic epithelia in the Pkd2WS25/- mouse model of ADPKD. Am. J. Physiol. Renal Physiol. 285: F870-F880 [Abstract] [Full Text]
Ward, C. J., Yuan, D., Masyuk, T. V., Wang, X., Punyashthiti, R., Whelan, S., Bacallao, R., Torra, R., LaRusso, N. F., Torres, V. E., Harris, P. C. (2003). Cellular and subcellular localization of the ARPKD protein; fibrocystin is expressed on primary cilia. Hum Mol Genet 12: 2703-2710 [Abstract] [Full Text]
Baker, S. A., Freeman, K., Luby-Phelps, K., Pazour, G. J., Besharse, J. C. (2003). IFT20 Links Kinesin II with a Mammalian Intraflagellar Transport Complex That Is Conserved in Motile Flagella and Sensory Cilia. J. Biol. Chem. 278: 34211-34218 [Abstract] [Full Text]
Joly, D., Hummel, A., Ruello, A., Knebelmann, B. (2003). Ciliary function of polycystins: a new model for cystogenesis. Nephrol Dial Transplant 18: 1689-1692 [Full Text]
Brown, J. M., Fine, N. A., Pandiyan, G., Thazhath, R., Gaertig, J. (2003). Hypoxia Regulates Assembly of Cilia in Suppressors of Tetrahymena Lacking an Intraflagellar Transport Subunit Gene. Mol. Biol. Cell 14: 3192-3207 [Abstract] [Full Text]
Pan, J., Snell, W. J. (2003). Kinesin II and regulated intraflagellar transport of Chlamydomonas aurora protein kinase. J. Cell Sci. 116: 2179-2186 [Abstract] [Full Text]
Calvet, J. P. (2003). New insights into ciliary function: Kidney cysts and photoreceptors. Proc. Natl. Acad. Sci. USA 100: 5583-5585 [Full Text]
Gavet, O., Alvarez, C., Gaspar, P., Bornens, M. (2003). Centrin4p, a Novel Mammalian Centrin Specifically Expressed in Ciliated Cells. Mol. Biol. Cell 14: 1818-1834 [Abstract] [Full Text]
Perrone, C. A., Tritschler, D., Taulman, P., Bower, R., Yoder, B. K., Porter, M. E. (2003). A Novel Dynein Light Intermediate Chain Colocalizes with the Retrograde Motor for Intraflagellar Transport at Sites of Axoneme Assembly in Chlamydomonas and Mammalian Cells. Mol. Biol. Cell 14: 2041-2056 [Abstract] [Full Text]
Schafer, J. C., Haycraft, C. J., Thomas, J. H., Yoder, B. K., Swoboda, P. (2003). XBX-1 Encodes a Dynein Light Intermediate Chain Required for Retrograde Intraflagellar Transport and Cilia Assembly in Caenorhabditis elegans. Mol. Biol. Cell 14: 2057-2070 [Abstract] [Full Text]
Lin, F., Hiesberger, T., Cordes, K., Sinclair, A. M., Goldstein, L. S. B., Somlo, S., Igarashi, P. (2003). Kidney-specific inactivation of the KIF3A subunit of kinesin-II inhibits renal ciliogenesis and produces polycystic kidney disease. Proc. Natl. Acad. Sci. USA 100: 5286-5291 [Abstract] [Full Text]
Pfannenschmid, F., Wimmer, V. C., Rios, R.-M., Geimer, S., Krockel, U., Leiherer, A., Haller, K., Nemcova, Y., Mages, W. (2003). Chlamydomonas DIP13 and human NA14: a new class of proteins associated with microtubule structures is involved in cell division. J. Cell Sci. 116: 1449-1462 [Abstract] [Full Text]
Ibanez-Tallon, I., Heintz, N., Omran, H. (2003). To beat or not to beat: roles of cilia in development and disease. Hum Mol Genet 12: R27-35 [Abstract] [Full Text]
Hill, K. L. (2003). Biology and Mechanism of Trypanosome Cell Motility. Eukaryot Cell 2: 200-208 [Full Text]