Nmd3p Is a Crm1p-Dependent Adapter Protein for Nuclear Export of the Large Ribosomal Subunit

doi:10.1083/jcb.151.5.1057

Published online 27 November 2000. doi:10.1083/jcb.151.5.1057

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© The Rockefeller University Press, 0021-9525/2000//1057 $5.00
The Journal of Cell Biology, Volume 151, Number 5, , 2000 1057-1066

Original Article

Nmd3p Is a Crm1p-Dependent Adapter Protein for Nuclear Export of the Large Ribosomal Subunit

Jennifer Hei-Ngam Ho^a, George Kallstrom^a, and Arlen W. Johnson^a

^a Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712
Section of Molecular Genetics and Microbiology, ESB325, The University of Texas at Austin, Austin, TX 78712-1095.(512) 471-7088(512) 475-6350

In eukaryotic cells, nuclear export of nascent ribosomal subunitsthrough the nuclear pore complex depends on the small GTPaseRan. However, neither the nuclear export signals (NESs) forthe ribosomal subunits nor the receptor proteins, which recognizethe NESs and mediate export of the subunits, have been identified.We showed previously that Nmd3p is an essential protein fromyeast that is required for a late step in biogenesis of thelarge (60S) ribosomal subunit. Here, we show that Nmd3p shuttlesand that deletion of the NES from Nmd3p leads to nuclear accumulationof the mutant protein, inhibition of the 60S subunit biogenesis,and inhibition of the nuclear export of 60S subunits. Moreover,the 60S subunits that accumulate in the nucleus can be coimmunoprecipitatedwith the NES-deficient Nmd3p. 60S subunit biogenesis and exportof truncated Nmd3p were restored by the addition of an exogenousNES. To identify the export receptor for Nmd3p we show thatNmd3p shuttling and 60S export is blocked by the Crm1p-specificinhibitor leptomycin B. These results identify Crm1p as thereceptor for Nmd3p export. Thus, export of the 60S subunit ismediated by the adapter protein Nmd3p in a Crm1p-dependent pathway.

Key Words: nuclear export • ribosome • Crm1p • Nmd3p • Saccharomyces cerevisiae

Abbreviations used in this paper: aa, amino acid; DAPI, 4'-6'-diaminodino-2-phenylindole;GFP, green fluorescent protein; NES, nuclear export signal;NLS, nuclear localization sequence; oligo; oligonucleotide;PKI, cAMP-dependent protein kinase inhibitor.

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