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© The Rockefeller University Press, 0021-9525/2000//1337 $5.00
The Journal of Cell Biology, Volume 151, Number 6, , 2000 1337-1344

The Cortical Protein Num1p Is Essential for Dynein-Dependent Interactions of Microtubules with the Cortex

^a Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110
Department of Cell Biology and Physiology, Washington University School of Medicine, 660 South Euclid Ave., Box 8228, St. Louis, MO 63110.(314) 362-0098(314) 362-3964

jcooper{at}cellbio.wustl.edu

In budding yeast, the mitotic spindle moves into the neck between the mother and bud via dynein-dependent sliding of cytoplasmic microtubules along the cortex of the bud. How dynein and microtubules interact with the cortex is unknown. We found that cells lacking Num1p failed to exhibit dynein-dependent microtubule sliding in the bud, resulting in defective mitotic spindle movement and nuclear segregation. Num1p localized to the bud cortex, and that localization was independent of microtubules, dynein, or dynactin. These data are consistent with Num1p being an essential element of the cortical attachment mechanism for dynein-dependent sliding of microtubules in the bud.

Key Words: microtubule attachment • dynein • mitosis • Num1p • yeast

© 2000 The Rockefeller University Press

Richard A. Heil-Chapdelaine's current address is St. Cloud State University, Department of Biological Sciences, MS 230, 720 4th Street N., St. Cloud, MN 56301-4498.

Abbreviations used in this paper: HU, hydroxyurea.

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