Report

The Cortical Protein Num1p Is Essential for Dynein-dependent Interactions of Microtubules with the Cortex

Correspondence to: John A. Cooper, Department of Cell Biology and Physiology, Washington University School of Medicine, 660 South Euclid Ave., Box 8228, St. Louis, MO 63110. Tel:(314) 362-3964 Fax:(314) 362-0098 E-mail:jcooper{at}cellbio.wustl.edu.

In budding yeast, the mitotic spindle moves into the neck between the mother and bud via dynein-dependent sliding of cytoplasmic microtubules along the cortex of the bud. How dynein and microtubules interact with the cortex is unknown. We found that cells lacking Num1p failed to exhibit dynein-dependent microtubule sliding in the bud, resulting in defective mitotic spindle movement and nuclear segregation. Num1p localized to the bud cortex, and that localization was independent of microtubules, dynein, or dynactin. These data are consistent with Num1p being an essential element of the cortical attachment mechanism for dynein-dependent sliding of microtubules in the bud.

Key Words: microtubule attachment, dynein, mitosis, Num1p, yeast

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Amaro, I. A., Costanzo, M., Boone, C., Huffaker, T. C. (2008). The Saccharomyces cerevisiae Homolog of p24 Is Essential for Maintaining the Association of p150Glued With the Dynactin Complex. Genetics 178: 703-709 [Abstract] [Full Text]  
• Griffis, E. R., Stuurman, N., Vale, R. D. (2007). Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. JCB 177: 1005-1015 [Abstract] [Full Text]  
• Yamashita, A., Yamamoto, M. (2006). Fission Yeast Num1p Is a Cortical Factor Anchoring Dynein and Is Essential for the Horse-Tail Nuclear Movement During Meiotic Prophase. Genetics 173: 1187-1196 [Abstract] [Full Text]  
• Fink, G., Steinberg, G. (2006). Dynein-dependent Motility of Microtubules and Nucleation Sites Supports Polarization of the Tubulin Array in the Fungus Ustilago maydis. Mol. Biol. Cell 17: 3242-3253 [Abstract] [Full Text]  
• Saito, T. T., Okuzaki, D., Nojima, H. (2006). Mcp5, a meiotic cell cortex protein, is required for nuclear movement mediated by dynein and microtubules in fission yeast. JCB 173: 27-33 [Abstract] [Full Text]  
• Moore, J. K., D'Silva, S., Miller, R. K. (2006). The CLIP-170 Homologue Bik1p Promotes the Phosphorylation and Asymmetric Localization of Kar9p. Mol. Biol. Cell 17: 178-191 [Abstract] [Full Text]  
• Li, S., Oakley, C. E., Chen, G., Han, X., Oakley, B. R., Xiang, X. (2005). Cytoplasmic Dynein's Mitotic Spindle Pole Localization Requires a Functional Anaphase-promoting Complex, {gamma}-Tubulin, and NUDF/LIS1 in Aspergillus nidulans. Mol. Biol. Cell 16: 3591-3605 [Abstract] [Full Text]  
• Huisman, S. M., Segal, M. (2005). Cortical capture of microtubules and spindle polarity in budding yeast - where's the catch?. J. Cell Sci. 118: 463-471 [Abstract] [Full Text]  
• Malinska, K., Malinsky, J., Opekarova, M., Tanner, W. (2004). Distribution of Can1p into stable domains reflects lateral protein segregation within the plasma membrane of living S. cerevisiae cells. J. Cell Sci. 117: 6031-6041 [Abstract] [Full Text]  
• Huisman, S. M., Bales, O. A.M., Bertrand, M., Smeets, M. F.M.A., Reed, S. I., Segal, M. (2004). Differential contribution of Bud6p and Kar9p to microtubule capture and spindle orientation in S. cerevisiae. JCB 167: 231-244 [Abstract] [Full Text]  
• Fraschini, R., Bilotta, D., Lucchini, G., Piatti, S. (2004). Functional Characterization of Dma1 and Dma2, the Budding Yeast Homologues of Schizosaccharomyces pombe Dma1 and Human Chfr. Mol. Biol. Cell 15: 3796-3810 [Abstract] [Full Text]  
• Yamamoto, A., Hiraoka, Y. (2003). Cytoplasmic dynein in fungi: insights from nuclear migration. J. Cell Sci. 116: 4501-4512 [Abstract] [Full Text]  
• Goehring, A. S., Mitchell, D. A., Tong, A. H. Y., Keniry, M. E., Boone, C., Sprague, G. F. Jr. (2003). Synthetic Lethal Analysis Implicates Ste20p, a p21-activated Protein Kinase, in Polarisome Activation. Mol. Biol. Cell 14: 1501-1516 [Abstract] [Full Text]  
• Efimov, V. P. (2003). Roles of NUDE and NUDF Proteins of Aspergillus nidulans: Insights from Intracellular Localization and Overexpression Effects. Mol. Biol. Cell 14: 871-888 [Abstract] [Full Text]  
• Xiang, X. (2003). LIS1 at the microtubule plus end and its role in dynein-mediated nuclear migration. JCB 160: 289-290 [Abstract] [Full Text]  
• Lee, W.-L., Oberle, J. R., Cooper, J. A. (2003). The role of the lissencephaly protein Pac1 during nuclear migration in budding yeast. JCB 160: 355-364 [Abstract] [Full Text]  
• Dimmer, K. S., Fritz, S., Fuchs, F., Messerschmitt, M., Weinbach, N., Neupert, W., Westermann, B. (2002). Genetic Basis of Mitochondrial Function and Morphology in Saccharomyces cerevisiae. Mol. Biol. Cell 13: 847-853 [Abstract] [Full Text]  
• Young, M. E., Karpova, T. S., Brugger, B., Moschenross, D. M., Wang, G. K., Schneiter, R., Wieland, F. T., Cooper, J. A. (2002). The Sur7p Family Defines Novel Cortical Domains in Saccharomyces cerevisiae, Affects Sphingolipid Metabolism, and Is Involved in Sporulation. Mol. Cell. Biol. 22: 927-934 [Abstract] [Full Text]  
• Caron, J. M., Vega, L. R., Fleming, J., Bishop, R., Solomon, F. (2001). Single Site alpha -Tubulin Mutation Affects Astral Microtubules and Nuclear Positioning during Anaphase in Saccharomyces cerevisiae: Possible Role for Palmitoylation of alpha -Tubulin. Mol. Biol. Cell 12: 2672-2687 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents