Dual Stimulation of Ras/Mitogen-activated Protein Kinase and RhoA by Cell Adhesion to Fibronectin Supports Growth Factor-stimulated Cell Cycle Progression

doi:10.1083/jcb.151.7.1413

Published online 18 December 2000. doi:10.1083/jcb.151.7.1413

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© The Rockefeller University Press, 0021-9525/2000/12/1413/ $5.00
The Journal of Cell Biology, Volume 151, Number 7, December 25, 2000 1413-1422

Original Article

Dual Stimulation of Ras/Mitogen-activated Protein Kinase and RhoA by Cell Adhesion to Fibronectin Supports Growth Factor–stimulated Cell Cycle Progression

Erik H.J. Danen^a,b, Petra Sonneveld^a, Arnoud Sonnenberg^a, and Kenneth M. Yamada^b
^a Division of Cell Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
^b Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892-4370

Correspondence to: Erik H.J. Danen, Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands, Tel:(3120) 512-1934 Fax:(3120) 512-1944 E-mail:edanen{at}nki.nl.

In cellular transformation, activated forms of the small GTPasesRas and RhoA can cooperate to drive cells through the G1 phaseof the cell cycle. Here, we show that a similar but substrate-regulatedmechanism is involved in the anchorage-dependent proliferationof untransformed NIH-3T3 cells. Among several extracellularmatrix components tested, only fibronectin supported growthfactor–induced, E2F-dependent S phase entry. Althoughall substrates supported the mitogen-activated protein kinase(MAPK) response to growth factors, RhoA activity was specificallyenhanced on fibronectin. Moreover, induction of cyclin D1 andsuppression of p21^Cip/Waf occurred specifically, in a Rho-dependentfashion, in cells attached to fibronectin. This ability of fibronectinto stimulate both Ras/MAPK- and RhoA-dependent signaling canexplain its potent cooperation with growth factors in the stimulationof cell cycle progression.

Key Words: fibronectin, cell adhesion, G1 cell cycle, integrin, Rho

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