Human Survivin Is a Kinetochore-associated Passenger Protein

doi:10.1083/jcb.151.7.1575

Published online 27 December 2000. doi:10.1083/jcb.151.7.1575

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© The Rockefeller University Press, 0021-9525/2000/12/1575/ $5.00
The Journal of Cell Biology, Volume 151, Number 7, December 25, 2000 1575-1582

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Human Survivin Is a Kinetochore-associated Passenger Protein

Dimitrios A. Skoufias^a, Cristiana Mollinari^a, Françoise B. Lacroix^a, and Robert L. Margolis^a
^a Institut de Biologie Structurale Jean-Pierre Ebel (Commissariat á l'Energie Atomique–Centre National de la Recherche Scientifique), 38027 Grenoble Cedex 1, France

Correspondence to: Robert L. Margolis, Institut de Biologie Structurale Jean-Pierre Ebel (CEA-CNRS), 41 rue Jules Horowitz, 38027 Grenoble cedex 1, France. Tel:(33) 4 76 88 96 16 Fax:(33) 4 76 88 54 94 E-mail:margolis{at}ibs.fr.

Survivin, a dimeric baculovirus inhibitor of apoptosis repeat(BIR) motif protein that is principally expressed in G2 andmitosis, has been associated with protection against apoptosisof cells that exit mitosis aberrantly. Mammalian survivin hasbeen reported to associate with centrosomes and with the mitoticspindle. We have expressed a human hemagglutinin-tagged survivinplasmid to determine its localization, and find instead thatit clearly acts as a passenger protein. In HeLa cells, survivinfirst associates with the kinetochores, and then translocatesto the spindle midzone during anaphase and, finally, to themidbody during cell cleavage. Its localization is similar tothat of TD-60, a known passenger protein. Both a point mutationin the baculovirus IAP repeat motif (C84A) and a COOH-terminaldeletion mutant ( ${Delta}$ 106) of survivin fail to localize to eitherkinetochores or midbodies, but neither interferes with cellcleavage. The interphase localization of survivin is cell cycleregulated since in permanently transfected NIH3T3 cells it isexcluded from the nuclei until G2, where it localizes with centromeres.Survivin remains associated with mitotic kinetochores when microtubuleassembly is disrupted and its localization is thus independentof microtubules. We conclude that human survivin is positionedto have an important function in the mechanism of cell cleavage.

Key Words: survivin, mitosis, kinetochore, passenger protein, cytokinesis

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