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Published online 8 January 2001. doi:10.1083/jcb.152.1.231
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© The Rockefeller University Press, 0021-9525/2001//231 $5.00
The Journal of Cell Biology, Volume 152, Number 1, , 2001 231-236

Report

Tangled1

: A Microtubule Binding Protein Required for the Spatial Control of Cytokinesis in Maize



Laurie G. Smitha, Suzanne M. Gerttulaa, Shengcheng Hana, and Joshua Levyb

a Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, California 92093
b Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
Section of Cell and Developmental Biology, University of California at San Diego, 9500 Gilman Dr., La Jolla, CA 92093-0116.(858) 534-7108(858) 822-2531

lsmith{at}biomail.ucsd.edu

Spatial control of cytokinesis in plant cells depends on guidance of the cytokinetic apparatus, the phragmoplast, to a cortical "division site" established before mitosis. Previously, we showed that the Tangled1 (Tan1) gene of maize is required for this process during maize leaf development (Cleary, A.L., and L.G. Smith. 1998. Plant Cell. 10:1875–1888.). Here, we show that the Tan1 gene is expressed in dividing cells and encodes a highly basic protein that can directly bind to microtubules (MTs). Moreover, proteins recognized by anti-TAN1 antibodies are preferentially associated with the MT-containing cytoskeletal structures that are misoriented in dividing cells of tan1 mutants. These results suggest that TAN1 protein participates in the orientation of cytoskeletal structures in dividing cells through an association with MTs.

Key Words: cytokinesis • phragmoplast • preprophase band • microtubules • plant cytoskeleton

© 2001 The Rockefeller University Press

The online version of this article contains supplemental material.

Abbreviations used in this paper: APC, adenomatous polyposis coli; MT, microtubule; PPB, preprophase band.


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