The Ndc80p Complex from Saccharomyces cerevisiae Contains Conserved Centromere Components and Has a Function in Chromosome Segregation

doi:10.1083/jcb.152.2.349

Published online 22 January 2001. doi:10.1083/jcb.152.2.349

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© The Rockefeller University Press, 0021-9525/2001/1/349/ $5.00
The Journal of Cell Biology, Volume 152, Number 2, January 22, 2001 349-360

Original Article

The Ndc80p Complex from Saccharomyces cerevisiae Contains Conserved Centromere Components and Has a Function in Chromosome Segregation

Philip A. Wigge^a and John V. Kilmartin^a
^a Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom

Correspondence to: John V. Kilmartin, MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK. Tel:44-1223-402-242 Fax:44-1223-412-142 E-mail:jvk{at}mrc-lmb.cam.ac.uk.

We have purified a complex from Saccharomyces cerevisiae containingthe spindle components Ndc80p, Nuf2p, Spc25p, and Spc24p. Temperature-sensitivemutants in NDC80, SPC25, and SPC24 show defects in chromosomesegregation. In spc24-1 cells, green fluorescence protein (GFP)-labeledcentromeres fail to split during spindle elongation, and inaddition some centromeres may detach from the spindle. Chromatinimmunoprecipitation assays show an association of all four componentsof the complex with the yeast centromere. Homologues of Ndc80p,Nuf2p, and Spc24p were found in Schizosaccharomyces pombe andGFP tagging showed they were located at the centromere. A humanhomologue of Nuf2p was identified in the expressed sequencetag database. Immunofluorescent staining with anti–humanNuf2p and with anti-HEC, the human homologue of Ndc80p, showedthat both proteins are at the centromeres of mitotic HeLa cells.Thus the Ndc80p complex contains centromere-associated componentsconserved between yeasts and vertebrates.

Key Words: Ndc80p, Nuf2p, Spc25p, Spc24p, centromere

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Tournier, S., Gachet, Y., Buck, V., Hyams, J. S., Millar, J. B.A. (2004). Disruption of Astral Microtubule Contact with the Cell Cortex Activates a Bub1, Bub3, and Mad3-dependent Checkpoint in Fission Yeast. Mol. Biol. Cell 15: 3345-3356 [Abstract] [Full Text]
Bharadwaj, R., Qi, W., Yu, H. (2004). Identification of Two Novel Components of the Human NDC80 Kinetochore Complex. J. Biol. Chem. 279: 13076-13085 [Abstract] [Full Text]
Borkovich, K. A., Alex, L. A., Yarden, O., Freitag, M., Turner, G. E., Read, N. D., Seiler, S., Bell-Pedersen, D., Paietta, J., Plesofsky, N., Plamann, M., Goodrich-Tanrikulu, M., Schulte, U., Mannhaupt, G., Nargang, F. E., Radford, A., Selitrennikoff, C., Galagan, J. E., Dunlap, J. C., Loros, J. J., Catcheside, D., Inoue, H., Aramayo, R., Polymenis, M., Selker, E. U., Sachs, M. S., Marzluf, G. A., Paulsen, I., Davis, R., Ebbole, D. J., Zelter, A., Kalkman, E. R., O'Rourke, R., Bowring, F., Yeadon, J., Ishii, C., Suzuki, K., Sakai, W., Pratt, R. (2004). Lessons from the Genome Sequence of Neurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism. Microbiol. Mol. Biol. Rev. 68: 1-108 [Abstract] [Full Text]
Gillett, E. S., Espelin, C. W., Sorger, P. K. (2004). Spindle checkpoint proteins and chromosome-microtubule attachment in budding yeast. JCB 164: 535-546 [Abstract] [Full Text]
Tien, A.-C., Lin, M.-H., Su, L.-J., Hong, Y.-R., Cheng, T.-S., Lee, Y.-C. G., Lin, W.-J., Still, I. H., Huang, C.-Y. F. (2004). Identification of the Substrates and Interaction Proteins of Aurora Kinases from a Protein-Protein Interaction Model. Mol. Cell. Proteomics 3: 93-104 [Abstract] [Full Text]
De Wulf, P., McAinsh, A. D., Sorger, P. K. (2003). Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev. 17: 2902-2921 [Abstract] [Full Text]
Nekrasov, V. S., Smith, M. A., Peak-Chew, S., Kilmartin, J. V. (2003). Interactions between Centromere Complexes in Saccharomyces cerevisiae. Mol. Biol. Cell 14: 4931-4946 [Abstract] [Full Text]
Westermann, S., Cheeseman, I. M., Anderson, S., Yates, J. R. III, Drubin, D. G., Barnes, G. (2003). Architecture of the budding yeast kinetochore reveals a conserved molecular core. JCB 163: 215-222 [Abstract] [Full Text]
Desai, A., Rybina, S., Muller-Reichert, T., Shevchenko, A., Shevchenko, A., Hyman, A., Oegema, K. (2003). KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. Genes Dev. 17: 2421-2435 [Abstract] [Full Text]
Appelgren, H., Kniola, B., Ekwall, K. (2003). Distinct centromere domain structures with separate functions demonstrated in live fission yeast cells. J. Cell Sci. 116: 4035-4042 [Abstract] [Full Text]
Kilmartin, J. V. (2003). Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication. JCB 162: 1211-1221 [Abstract] [Full Text]
Le Masson, I., Yu, D. Y., Jensen, K., Chevalier, A., Courbeyrette, R., Boulard, Y., Smith, M. M., Mann, C. (2003). Yaf9, a Novel NuA4 Histone Acetyltransferase Subunit, Is Required for the Cellular Response to Spindle Stress in Yeast. Mol. Cell. Biol. 23: 6086-6102 [Abstract] [Full Text]
Hori, T., Haraguchi, T., Hiraoka, Y., Kimura, H., Fukagawa, T. (2003). Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells. J. Cell Sci. 116: 3347-3362 [Abstract] [Full Text]
Shang, C., Hazbun, T. R., Cheeseman, I. M., Aranda, J., Fields, S., Drubin, D. G., Barnes, G. (2003). Kinetochore Protein Interactions and their Regulation by the Aurora Kinase Ipl1p. Mol. Biol. Cell 14: 3342-3355 [Abstract] [Full Text]
Lu, L., Arakaki, A. K., Lu, H., Skolnick, J. (2003). Multimeric Threading-Based Prediction of Protein-Protein Interactions on a Genomic Scale: Application to the Saccharomyces cerevisiae Proteome. Genome Res 13: 1146-1154 [Abstract] [Full Text]
Pidoux, A. L., Richardson, W., Allshire, R. C. (2003). Sim4: a novel fission yeast kinetochore protein required for centromeric silencing and chromosome segregation. JCB 161: 295-307 [Abstract] [Full Text]
Kemp, H. A., Sprague, G. F. Jr. (2003). Far3 and Five Interacting Proteins Prevent Premature Recovery from Pheromone Arrest in the Budding Yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 23: 1750-1763 [Abstract] [Full Text]
Pot, I., Measday, V., Snydsman, B., Cagney, G., Fields, S., Davis, T. N., Muller, E. G.D., Hieter, P. (2003). Chl4p and Iml3p Are Two New Members of the Budding Yeast Outer Kinetochore. Mol. Biol. Cell 14: 460-476 [Abstract] [Full Text]
McCleland, M. L., Gardner, R. D., Kallio, M. J., Daum, J. R., Gorbsky, G. J., Burke, D. J., Stukenberg, P. T. (2003). The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. 17: 101-114 [Abstract] [Full Text]
DeLuca, J. G., Moree, B., Hickey, J. M., Kilmartin, J. V., Salmon, E.D. (2002). hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells. JCB 159: 549-555 [Abstract] [Full Text]
Martin-Lluesma, S., Stucke, V. M., Nigg, E. A. (2002). Role of Hec1 in Spindle Checkpoint Signaling and Kinetochore Recruitment of Mad1/Mad2. Science 297: 2267-2270 [Abstract] [Full Text]
Mehta, S., Yang, X. M., Chan, C. S., Dobson, M. J., Jayaram, M., Velmurugan, S. (2002). The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?. JCB 158: 625-637 [Abstract] [Full Text]
Abruzzi, K. C., Magendantz, M., Solomon, F. (2002). An {alpha}-Tubulin Mutant Demonstrates Distinguishable Functions Among the Spindle Assembly Checkpoint Genes in Saccharomyces cerevisiae. Genetics 161: 983-994 [Abstract] [Full Text]
Stemmann, O., Neidig, A., Kocher, T., Wilm, M., Lechner, J. (2002). Hsp90 enables Ctf13p/Skp1p to nucleate the budding yeast kinetochore. Proc. Natl. Acad. Sci. USA 99: 8585-8590 [Abstract] [Full Text]
Bloom, K. (2002). Yeast weighs in on the elusive spindle matrix: New filaments in the nucleus. Proc. Natl. Acad. Sci. USA 99: 4757-4759 [Full Text]
van Hemert, M. J., Lamers, G. E. M., Klein, D. C. G., Oosterkamp, T. H., Steensma, H. Y., van Heusden, G. P. H. (2002). From the Cover: The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies. Proc. Natl. Acad. Sci. USA 99: 5390-5393 [Abstract] [Full Text]
Cheeseman, I. M., Drubin, D. G., Barnes, G. (2002). Simple centromere, complex kinetochore: linking spindle microtubules and centromeric DNA in budding yeast. JCB 157: 199-203 [Abstract] [Full Text]
Euskirchen, G. M. (2002). Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a New Group of Proteins Important for Chromosome Segregation in Saccharomyces cerevisiae. Eukaryot Cell 1: 229-240 [Abstract] [Full Text]
Petersen, J., Paris, J., Willer, M., Philippe, M., Hagan, I. M. (2002). The S. pombe aurora-related kinase Ark1 associates with mitotic structures in a stage dependent manner and is required for chromosome segregation. J. Cell Sci. 114: 4371-4384 [Abstract] [Full Text]
Castillo, A. R., Meehl, J. B., Morgan, G., Schutz-Geschwender, A., Winey, M. (2002). The yeast protein kinase Mps1p is required for assembly of the integral spindle pole body component Spc42p. JCB 156: 453-465 [Abstract] [Full Text]
West, R. R., Malmstrom, T., McIntosh, J. R. (2002). Kinesins klp5+ and klp6+ are required for normal chromosome movement in mitosis. J. Cell Sci. 115: 931-940 [Abstract] [Full Text]
Wang, S.-W., Read, R. L., Norbury, C. J. (2002). Fission yeast Pds5 is required for accurate chromosome segregation and for survival after DNA damage or metaphase arrest. J. Cell Sci. 115: 587-598 [Abstract] [Full Text]
Measday, V., Hailey, D. W., Pot, I., Givan, S. A., Hyland, K. M., Cagney, G., Fields, S., Davis, T. N., Hieter, P. (2002). Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore. Genes Dev. 16: 101-113 [Abstract] [Full Text]
Jones, M. H., He, X., Giddings, T. H., Winey, M. (2001). Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle. Proc. Natl. Acad. Sci. USA 10.1073/pnas.241417098v1 [Abstract] [Full Text]
Kniola, B., O'Toole, E., McIntosh, J. R., Mellone, B., Allshire, R., Mengarelli, S., Hultenby, K., Ekwall, K. (2001). The Domain Structure of Centromeres Is Conserved from Fission Yeast to Humans. Mol. Biol. Cell 12: 2767-2775 [Abstract] [Full Text]
Dernburg, A. F. (2001). Here, There, and Everywhere: Kinetochore Function on Holocentric Chromosomes. JCB 153: F33-F38 [Abstract] [Full Text]
Howe, M., McDonald, K. L., Albertson, D. G., Meyer, B. J. (2001). HIM-10 Is Required for Kinetochore Structure and Function on Caenorhabditis elegans Holocentric Chromosomes. JCB 153: 1227-1238 [Abstract] [Full Text]
Pearson, C. G., Maddox, P. S., Salmon, E.D., Bloom, K. (2001). Budding Yeast Chromosome Structure and Dynamics during Mitosis. JCB 152: 1255-1266 [Abstract] [Full Text]
van Hemert, M. J., Lamers, G. E. M., Klein, D. C. G., Oosterkamp, T. H., Steensma, H. Y., van Heusden, G. P. H. (2002). From the Cover: The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies. Proc. Natl. Acad. Sci. USA 99: 5390-5393 [Abstract] [Full Text]
Jones, M. H., He, X., Giddings, T. H., Winey, M. (2001). Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle. Proc. Natl. Acad. Sci. USA 98: 13675-13680 [Abstract] [Full Text]
Kang, J.-s., Cheeseman, I. M., Kallstrom, G., Velmurugan, S., Barnes, G., Chan, C. S.M. (2001). Functional cooperation of Dam1, Ipl1, and the inner centromere protein (INCENP)-related protein Sli15 during chromosome segregation. JCB 155: 763-774 [Abstract] [Full Text]
Lin, H., de Carvalho, P., Kho, D., Tai, C.-Y., Pierre, P., Fink, G. R., Pellman, D. (2001). Polyploids require Bik1 for kinetochore-microtubule attachment. JCB 155: 1173-1184 [Abstract] [Full Text]
Cheeseman, I. M., Brew, C., Wolyniak, M., Desai, A., Anderson, S., Muster, N., Yates, J. R., Huffaker, T. C., Drubin, D. G., Barnes, G. (2001). Implication of a novel multiprotein Dam1p complex in outer kinetochore function. JCB 155: 1137-1146 [Abstract] [Full Text]
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