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Published online 5 February 2001. doi:10.1083/jcb.152.3.451
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© The Rockefeller University Press, 0021-9525/2001//451 $5.00
The Journal of Cell Biology, Volume 152, Number 3, , 2001 451-470

Original Article

A Novel Function of Saccharomyces cerevisiae CDC5 in Cytokinesis



Sukgil Songa and Kyung S. Leea

a Laboratory of Metabolism, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
Laboratory of Metabolism, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bldg. 37, Rm. 3D25, Bethesda, MD 20892.(301) 496-8419(301) 496-9635

kyunglee{at}pop.nci.nih.gov

Coordination of mitotic exit with timely initiation of cytokinesis is critical to ensure completion of mitotic events before cell division. The Saccharomyces cerevisiae polo kinase Cdc5 functions in a pathway leading to the degradation of mitotic cyclin Clb2, thereby permitting mitotic exit. Here we provide evidence that Cdc5 also plays a role in regulating cytokinesis and that an intact polo-box, a conserved motif in the noncatalytic COOH-terminal domain of Cdc5, is required for this event. Depletion of Cdc5 function leads to an arrest in cytokinesis. Overexpression of the COOH-terminal domain of Cdc5 (cdc5{Delta}N), but not the corresponding polo-box mutant, resulted in connected cells. These cells shared cytoplasms with incomplete septa, and possessed aberrant septin ring structures. Provision of additional copies of endogenous CDC5 remedied this phenotype, suggesting a dominant-negative inhibition of cytokinesis. The polo-box–dependent interactions between Cdc5 and septins (Cdc11 and Cdc12) and genetic interactions between the dominant-negative cdc5{Delta}N and Cyk2/Hof1 or Myo1 suggest that direct interactions between cdc5{Delta}N and septins resulted in inhibition of Cyk2/Hof1- and Myo1-mediated cytokinetic pathways. Thus, we propose that Cdc5 may coordinate mitotic exit with cytokinesis by participating in both anaphase promoting complex activation and a polo-box–dependent cytokinetic pathway.

Key Words: Cdc5 • polo-box • mitosis • cytokinesis • septin

© 2001 The Rockefeller University Press

Abbreviations used in this paper: APC, anaphase promoting complex; GFP, green fluorescent protein; HA, hemagglutinin.


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