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Published online 5 February 2001. doi:10.1083/jcb.152.3.531
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© The Rockefeller University Press, 0021-9525/2001//531 $5.00
The Journal of Cell Biology, Volume 152, Number 3, , 2001 531-544

Original Article

The Cytokinesis Gene KEULE Encodes a Sec1 Protein That Binds the Syntaxin Knolle



Farhah F. Assaada, Yoann Huetb, Ulrike Mayerc, and Gerd Jürgensc

a Genetics and Microbiology Institute, Ludwig Maximilians University, D-80638 Munich, Germany
b Plant Science Institute, ETH-Zürich, CH 8092 Zürich, Switzerland
c Department of Developmental Genetics, University of Tübingen, D-72076 Tübingen, Germany
Carnegie Institution of Washington, 260 Panama St., Stanford, CA 94305.(650) 325-1521 ext

KEULE is required for cytokinesis in Arabidopsis thaliana. We have positionally cloned the KEULE gene and shown that it encodes a Sec1 protein. KEULE is expressed throughout the plant, yet appears enriched in dividing tissues. Cytokinesis-defective mutant sectors were observed in all somatic tissues upon transformation of wild-type plants with a KEULE–green fluorescent protein gene fusion, suggesting that KEULE is required not only during embryogenesis, but at all stages of the plant's life cycle. KEULE is characteristic of a Sec1 protein in that it appears to exist in two forms: soluble or peripherally associated with membranes. More importantly, KEULE binds the cytokinesis-specific syntaxin KNOLLE. Sec1 proteins are key regulators of vesicle trafficking, capable of integrating a large number of intra- and/or intercellular signals. As a cytokinesis-related Sec1 protein, KEULE appears to represent a novel link between cell cycle progression and the membrane fusion apparatus.

Key Words: cytokinesis • Sec1 • cell plate • syntaxin • Arabidopsis

© 2001 The Rockefeller University Press

Abbreviations used in this paper: BAC, bacterial artificial chromosome; CTAB, cetyltrimethylammonium bromide; GFP, green fluorescent protein; GST, glutathione S-transferase; RFLP, restriction fragment length polymorphism; RT, reverse transcription; YAC, yeast artificial chromosome.


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