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Published online 5 March 2001. doi:10.1083/jcb.152.5.1099
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© The Rockefeller University Press, 0021-9525/2001//1099 $5.00
The Journal of Cell Biology, Volume 152, Number 5, , 2001 1099-1106

Report

The Transcription Coactivator Cbp Is a Dynamic Component of the Promyelocytic Leukemia Nuclear Body



François-Michel Boisverta, Michael J. Kruhlaka, Alan K. Boxa, Michael J. Hendzelb, and David P. Bazett-Jonesa

a Department of Cell Biology and Anatomy, University of Calgary, Calgary, Alberta T2N 4N1, Canada
b Cross Cancer Institute, University of Alberta, Edmonton, Alberta T6G 1Z2, Canada
Department of Cell Biology and Anatomy, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB, T2N 4N1 Canada.(403) 270-0737(403) 220-3025

bazett{at}ucalgary.ca

The transcription coactivator and histone acetyltransferase CAMP response element–binding protein (CBP) has been demonstrated to accumulate in promyelocytic leukemia (PML) bodies. We show that this accumulation is cell type specific. In cells where CBP does not normally accumulate in PML bodies, it can be induced to accumulate in PML bodies through overexpression of either CBP or Pml, but not Sp100. Using fluorescence recovery after photobleaching, we demonstrate that CBP moves rapidly into and out of PML bodies. In contrast, Pml and Sp100 are relatively immobile in the nucleoplasm and within PML nuclear bodies. They possess the characteristics expected of proteins that would play a structural role in the integrity of these subnuclear domains. Our results are consistent with CBP being a dynamic component of PML bodies and that the steady-state level in these structures can be modulated by Pml.

Key Words: nuclear structure • promyelocytic leukemia • PML body • ND10 • fluorescence recovery after photobleaching

© 2001 The Rockefeller University Press

Abbreviations used in this paper: CBP, CAMP response element–binding protein; GFP, green fluorescent protein; PML, promyelocytic leukemia.


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