The Golgi-Associated Hook3 Protein Is a Member of a Novel Family of Microtubule-Binding Proteins

doi:10.1083/jcb.152.5.923

Published online 5 March 2001. doi:10.1083/jcb.152.5.923

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© The Rockefeller University Press, 0021-9525/2001//923 $5.00
The Journal of Cell Biology, Volume 152, Number 5, , 2001 923-934

Original Article

The Golgi-Associated Hook3 Protein Is a Member of a Novel Family of Microtubule-Binding Proteins

Jason H. Walenta^a, Aaron J. Didier^a, Xinran Liu^a, and Helmut Krämer^a

^a Center for Basic Neuroscience and Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390
Center for Basic Neuroscience and Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235-9111.(214) 648-1801(214) 648-1860

kramer{at}utsw.swmed.edu

Microtubules are central to the spatial organization of diversemembrane-trafficking systems. Here, we report that Hook proteinsconstitute a novel family of cytosolic coiled coil proteinsthat bind to organelles and to microtubules. The conserved NH₂-terminaldomains of Hook proteins mediate attachment to microtubules,whereas the more divergent COOH-terminal domains mediate thebinding to organelles. Human Hook3 bound to Golgi membranesin vitro and was enriched in the cis-Golgi in vivo. Unlike othercis-Golgi–associated proteins, however, a large fractionof Hook3 maintained its juxtanuclear localization after BrefeldinA treatment, indicating a Golgi-independent mechanism for Hook3localization. Because overexpression of Hook3 caused fragmentationof the Golgi complex, we propose that Hook3 participates indefining the architecture and localization of the mammalianGolgi complex.

Key Words: membrane trafficking • Hook protein • endosomes • brefeldin A • Golgi complex

Abbreviations used in this paper: aa, amino acids; BfA, BrefeldinA; CLIP, cytoplasmic linker protein; dHK, Drosophila Hook protein;hHK1, human Hook1 protein; hHK2, human Hook2 protein; hHK3,human Hook3 protein; MTOC, microtubule organizing center; MVB,multivesicular body; NZ, nocodazole; RT, room temperature.

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