JCB logo
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 19 March 2001. doi:10.1083/jcb.152.6.1255
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 586K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pearson, C. G.
Right arrow Articles by Bloom, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pearson, C. G.
Right arrow Articles by Bloom, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/2001//1255 $5.00
The Journal of Cell Biology, Volume 152, Number 6, , 2001 1255-1266

Original Article

Budding Yeast Chromosome Structure and Dynamics during Mitosis



Chad G. Pearsona, Paul S. Maddoxa, E.D. Salmona, and Kerry Blooma

a Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
Department of Biology, Fordham Hall 622, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280.(919) 962-8461(919) 962-2363

cgpearso{at}email.unc.edu

Using green fluorescent protein probes and rapid acquisition of high-resolution fluorescence images, sister centromeres in budding yeast are found to be separated and oscillate between spindle poles before anaphase B spindle elongation. The rates of movement during these oscillations are similar to those of microtubule plus end dynamics. The degree of preanaphase separation varies widely, with infrequent centromere reassociations observed before anaphase. Centromeres are in a metaphase-like conformation, whereas chromosome arms are neither aligned nor separated before anaphase. Upon spindle elongation, centromere to pole movement (anaphase A) was synchronous for all centromeres and occurred coincident with or immediately after spindle pole separation (anaphase B). Chromatin proximal to the centromere is stretched poleward before and during anaphase onset. The stretched chromatin was observed to segregate to the spindle pole bodies at rates greater than centromere to pole movement, indicative of rapid elastic recoil between the chromosome arm and the centromere. These results indicate that the elastic properties of DNA play an as of yet undiscovered role in the poleward movement of chromosome arms.

Key Words: budding yeast • motility • mitosis • chromosome dynamics • centromeres

© 2001 The Rockefeller University Press

Abbreviations used in this paper: CFP, cyan fluorescent protein; Cse4p, centromere-specific histone; GFP, green fluorescent protein; lacI, lac repressor; YFP, yellow fluorescent protein.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents