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© The Rockefeller University Press, 0021-9525/2001//111 $5.00
The Journal of Cell Biology, Volume 153, Number 1, , 2001 111-120

Ftsz Ring Formation at the Chloroplast Division Site in Plants

^a Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824
Department of Plant Biology, 166 Plant Biology Building, Michigan State University, East Lansing, MI 48824.(517) 353-1926(517) 355-4685

osteryou{at}msu.edu

Among the events that accompanied the evolution of chloroplasts from their endosymbiotic ancestors was the host cell recruitment of the prokaryotic cell division protein FtsZ to function in chloroplast division. FtsZ, a structural homologue of tubulin, mediates cell division in bacteria by assembling into a ring at the midcell division site. In higher plants, two nuclear-encoded forms of FtsZ, FtsZ1 and FtsZ2, play essential and functionally distinct roles in chloroplast division, but whether this involves ring formation at the division site has not been determined previously. Using immunofluorescence microscopy and expression of green fluorescent protein fusion proteins in Arabidopsis thaliana, we demonstrate here that FtsZ1 and FtsZ2 localize to coaligned rings at the chloroplast midpoint. Antibodies specific for recognition of FtsZ1 or FtsZ2 proteins in Arabidopsis also recognize related polypeptides and detect midplastid rings in pea and tobacco, suggesting that midplastid ring formation by FtsZ1 and FtsZ2 is universal among flowering plants. Perturbation in the level of either protein in transgenic plants is accompanied by plastid division defects and assembly of FtsZ1 and FtsZ2 into filaments and filament networks not observed in wild-type, suggesting that previously described FtsZ-containing cytoskeletal-like networks in chloroplasts may be artifacts of FtsZ overexpression.

Key Words: plastids • organelle replication • organelle fission • cytoskeleton • mitochondria

© 2001 The Rockefeller University Press

Abbreviations used in this paper: GFP, green fluorescent protein; CaMV, cauliflower mosaic virus; PD, plastid-dividing.

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