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© The Rockefeller University Press, 0021-9525/2001//159 $5.00
The Journal of Cell Biology, Volume 153, Number 1, , 2001 159-168

The Surveillance Mechanism of the Spindle Position Checkpoint in Yeast

^a Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110
Department of Cell Biology and Physiology, Washington University School of Medicine, 660 South Euclid Ave., St. Louis, MO 63110.(314) 362-0098(314) 362-4606

nadames{at}cellbio.wustl.edu

The spindle position checkpoint in Saccharomyces cerevisiae delays mitotic exit until the spindle has moved into the mother–bud neck, ensuring that each daughter cell inherits a nucleus. The small G protein Tem1p is critical in promoting mitotic exit and is concentrated at the spindle pole destined for the bud. The presumed nucleotide exchange factor for Tem1p, Lte1p, is concentrated in the bud. These findings suggested the hypothesis that movement of the spindle pole through the neck allows Tem1p to interact with Lte1p, promoting GTP loading of Tem1p and mitotic exit. However, we report that deletion of LTE1 had little effect on the timing of mitotic exit. We also examined several mutants in which some cells inappropriately exit mitosis even though the spindle is within the mother. In some of these cells, the spindle pole body did not interact with the bud or the neck before mitotic exit. Thus, some alternative mechanism must exist to coordinate mitotic exit with spindle position. In both wild-type and mutant cells, mitotic exit was preceded by loss of cytoplasmic microtubules from the neck. Thus, the spindle position checkpoint may monitor such interactions.

Key Words: checkpoint • mitosis • dynein • EBI • Saccharomyces cerevisiae

© 2001 The Rockefeller University Press

Abbreviations used in this paper: SEP, standard error of the proportion; SPB, spindle pole body.

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