Nucleolar Components Involved in Ribosome Biogenesis Cycle between the Nucleolus and Nucleoplasm in Interphase Cells

doi:10.1083/jcb.153.1.169

Published online 2 April 2001. doi:10.1083/jcb.153.1.169

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© The Rockefeller University Press, 0021-9525/2001/4/169/ $5.00
The Journal of Cell Biology, Volume 153, Number 1, April 2, 2001 169-176

Original Article

Nucleolar Components Involved in Ribosome Biogenesis Cycle between the Nucleolus and Nucleoplasm in Interphase Cells

Danyang Chen^a and Sui Huang^a
^a Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611

Correspondence to: Sui Huang, Dept. of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611. Tel:(312) 503-4269 Fax:(312) 503-7912 E-mail:s-huang2{at}northwestern.edu.

We examined the mobilities of nucleolar components that actat various steps of the ribosome biogenesis pathway. Fluorescencerecovery after photobleaching (FRAP) and fluorescence loss inphotobleaching (FLIP) analyses demonstrate that factors involvedin rRNA transcription (upstream-binding factor [UBF]), processing(nucleolin, fibrillarin, and RNase MRP subunits, Rpp29), andribosome assembly (B23) exchange rapidly between the nucleoplasmand nucleolus. In contrast, the mobilities of ribosomal subunitproteins (S5, L9) are much slower. Selective inhibition of RNApolymerase I transcription does not prevent the exchanges butinfluences the rates of exchange differentially for differentnucleolar components. These findings suggest that the rapidexchange of nucleolar components between the nucleolus and nucleoplasmmay represent a new level of regulation for rRNA synthesis.The different dynamic properties of proteins involved in differentsteps of ribosome biogenesis imply that the nucleolar associationof these proteins is due to their specific functional rolesrather than simply their specific nucleolar-targeting events.

Key Words: nucleolar proteins, ribosome biogenesis, dynamics, living cells

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