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Published online 2 April 2001. doi:10.1083/jcb.153.1.229
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© The Rockefeller University Press, 0021-9525/2001//229 $5.00
The Journal of Cell Biology, Volume 153, Number 1, , 2001 229-236

Report

Actin Bound to the Heterogeneous Nuclear Ribonucleoprotein Hrp36 Is Associated with Balbiani Ring mRNA from the Gene to Polysomes



Piergiorgio Percipallea, Jian Zhaoa, Brian Popeb, Alan Weedsb, Uno Lindbergc, and Bertil Daneholta

a Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, SE-17177 Stockholm, Sweden
b Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 2QH, United Kingdom
c Department of Zoological Cell Biology, Wenner-Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden
Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, SE-171 77 Stockholm, Sweden.46-8-31352946-8-7287370

bertil.daneholt{at}cmb.ki.se

In the salivary glands of the dipteran Chironomus tentans, a specific messenger ribonucleoprotein (mRNP) particle, the Balbiani ring (BR) granule, can be visualized during its assembly on the gene and during its nucleocytoplasmic transport. We now show with immunoelectron microscopy that actin becomes associated with the BR particle concomitantly with transcription and is present in the particle in the nucleoplasm. DNase I affinity chromatography experiments with extracts from tissue culture cells indicate that both nuclear and cytoplasmic actin are bound to the heterogeneous RNP (hnRNP) protein hrp36, but not to the hnRNP proteins hrp23 and hrp45. The interaction is likely to be direct as purified actin binds to recombinant hrp36 in vitro. Furthermore, it is demonstrated by cross linking that nuclear as well as cytoplasmic actin are bound to hrp36 in vivo. It is known that hrp36 is added cotranscriptionally along the BR mRNA molecule and accompanies the RNA through the nuclear pores and into polysomes. We conclude that actin is likely to be bound to the BR transcript via hrp36 during the transfer of the mRNA from the gene all the way into polysomes.

Key Words: actin • heterogeneous nuclear ribonucleoprotein • premessenger ribonucleoprotein particles • Balbiani rings • nucleocytoplasmic transport

© 2001 The Rockefeller University Press

Abbreviations used in this paper: BR, Balbiani ring; DSP, dithiobis-succinimidylpropionate; RNP, ribonucleoprotein.


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