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Published online 2 April 2001. doi:10.1083/jcb.153.1.237
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© The Rockefeller University Press, 0021-9525/2001//237 $5.00
The Journal of Cell Biology, Volume 153, Number 1, , 2001 237-242

Report

Centrosomes Enhance the Fidelity of Cytokinesis in Vertebrates and Are Required for Cell Cycle Progression



Alexey Khodjakova,b and Conly L. Riedera,b

a Laboratory of Cell Regulation, Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, New York 12201
b Department of Biomedical Sciences, State University of New York, Albany, New York 12222
Wadsworth Center, P.O. Box 509, Albany, NY 12201-0509.(518) 486-4901(518) 486-5339

khodj{at}wadsworth.org

When centrosomes are destroyed during prophase by laser microsurgery, vertebrate somatic cells form bipolar acentrosomal mitotic spindles (Khodjakov, A., R.W. Cole, B.R. Oakley, and C.L. Rieder. 2000. Curr. Biol. 10:59–67), but the fate of these cells is unknown. Here, we show that, although these cells lack the radial arrays of astral microtubules normally associated with each spindle pole, they undergo a normal anaphase and usually produce two acentrosomal daughter cells. Relative to controls, however, these cells exhibit a significantly higher (30–50%) failure rate in cytokinesis. This failure correlates with the inability of the spindle to properly reposition itself as the cell changes shape. Also, we destroyed just one centrosome during metaphase and followed the fate of the resultant acentrosomal and centrosomal daughter cells. Within 72 h, 100% of the centrosome-containing cells had either entered DNA synthesis or divided. By contrast, during this period, none of the acentrosomal cells had entered S phase. These data reveal that the primary role of the centrosome in somatic cells is not to form the spindle but instead to ensure cytokinesis and subsequent cell cycle progression.

Key Words: centrosome • cell cycle progression • cytokinesis • mitosis • vertebrates

© 2001 The Rockefeller University Press

The online version of this article contains supplemental material.

Abbreviations used in this paper: GFP, green fluorescent protein; Mt, microtubule.


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