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Published online 2 April 2001. doi:10.1083/jcb.153.1.47
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© The Rockefeller University Press, 0021-9525/2001//47 $5.00
The Journal of Cell Biology, Volume 153, Number 1, , 2001 47-62

Original Article

A Role for Actin, Cdc1p, and Myo2p in the Inheritance of Late Golgi Elements in Saccharomyces cerevisiae



Olivia W. Rossanesea, Catherine A. Reinkea, Brooke J. Bevisa, Adam T. Hammonda, Irina B. Searsa, James O'Connora, and Benjamin S. Glicka

a Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637
Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th St., Chicago, IL 60637.(773) 702-3172(773) 702-5315

bsglick{at}midway.uchicago.edu

In Saccharomyces cerevisiae, Golgi elements are present in the bud very early in the cell cycle. We have analyzed this Golgi inheritance process using fluorescence microscopy and genetics. In rapidly growing cells, late Golgi elements show an actin-dependent concentration at sites of polarized growth. Late Golgi elements are apparently transported into the bud along actin cables and are also retained in the bud by a mechanism that may involve actin. A visual screen for mutants defective in the inheritance of late Golgi elements yielded multiple alleles of CDC1. Mutations in CDC1 severely depolarize the actin cytoskeleton, and these mutations prevent late Golgi elements from being retained in the bud. The efficient localization of late Golgi elements to the bud requires the type V myosin Myo2p, further suggesting that actin plays a role in Golgi inheritance. Surprisingly, early and late Golgi elements are inherited by different pathways, with early Golgi elements localizing to the bud in a Cdc1p- and Myo2p-independent manner. We propose that early Golgi elements arise from ER membranes that are present in the bud. These two pathways of Golgi inheritance in S. cerevisiae resemble Golgi inheritance pathways in vertebrate cells.

Key Words: Golgi apparatus • endoplasmic reticulum • budding yeast • actins • myosin

© 2001 The Rockefeller University Press

Abbreviations used in this paper: COP, coat protein; DIC, differential interference contrast; EGFP, enhanced GFP; GFP, green fluorescent protein; LatA, latrunculin A; QSD, quasi-synthetic dextrose medium; tER, transitional ER.


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